METHYL GALLATE FROM JIRINGA (ARCHIDENDRON JIRINGA) AND ANTIOXIDANT ACTIVITY.

Misri Yanty Lubis; Rikson Siburian; Lamek Marpaung; Partomuan Simanjuntak; Muhammad Pandapotan Nasution

doi:10.22159/ajpcr.2018.v11i1.21637

Authors

Misri Yanty Lubis Department of Agrotechnology, Faculty of Agriculture, University of Graha Nusantara, Tor Simarsayang, Padangsidimpuan 22712, Indonesia.
Rikson Siburian Department of Chemistry, Faculty of Mathematic and Natural Science, University of Sumatera Utara, Padang Bulan, Medan 20155, Indonesia.
Lamek Marpaung Department of Chemistry, Faculty of Mathematic and Natural Science, University of Sumatera Utara, Padang Bulan, Medan 20155, Indonesia.
Partomuan Simanjuntak Department of Pharmacology, Faculty of Pharmacy, University of Pancasila, Srengseng Sawah, Jagakarsa, Jakarta 12630, Indonesia.
Muhammad Pandapotan Nasution Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Padang Bulan, Medan 20155, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i1.21637

Keywords:

Antioxidant, Archidendron jiringa, Methyl gallate, Nuclear magnetic resonance

Abstract

Â Objective: This research was study about phytochemical active phenolic compound from pods of jiringa (Archidendron jiringa (Jack) I. C. Nielsen) and it's antioxidant.

Methods: Pods of jiringa were air dried and macerated with methanol. Extract was evaporated using rotary evaporator, and then, crude extract dissolved with water and partitioned with ethyl acetate. Extracts were evaporated and partitioned again with methanol and n-hexane. Column and preparative chromatography used to separate pure compound. Pure compound as methyl gallate was identificated with data from nuclear magnetic resonances of proton H (1H NMR), NMR of carbon (13C NMR), and mass spectrometry (MS). Antioxidant activity of pure compound tested by 1,1-diphenyl-2-picrylhydrazyl method.

Results: We found pure phenolic compound from pods of jiringa as methyl gallate that exists on fraction III-2, and it has high antioxidant activity with inhibition concentration 50 was 3.7576 Î¼g/ml.

Conclusions: Pods of jiringa contain of active phenolic compound as methyl gallate that has high antioxidant activity. Therefore, it can be used as a source of natural antioxidant.

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Author Biography

Misri Yanty Lubis, Department of Agrotechnology, Faculty of Agriculture, University of Graha Nusantara, Tor Simarsayang, Padangsidimpuan 22712, Indonesia.

Chemistry Department

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