CELL CYCLE ARREST ACTIVITY OF LITSEA CUBEBA LOUR: HEARTWOOD AND FRUIT EXTRACTS AGAINST T47D BREAST CANCER CELLS
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i11.20204Keywords:
Cell cycle arrest, Litsea cubeba, Heartwood, Fruit, ExtractAbstract
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 Objective: This study was carried out to investigate cell cycle arrest activity toward T47D cells of Litsea cubeba heartwoods and fruits extract.
Methods: Dry extracts were prepared from dry-grounded heartwoods and fruits by cold maceration using n-hexane, ethylacetate, and ethanol solvent. Cytotoxic activity were measured by [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] methods and interpreted with inhibitory concentration 50% (IC50) value. Cell cycle arrest was investigated by flowcytometry method.
Results: IC50 values for each n-hexane, ethylacetate, and ethanol of L. cubeba heartwoods and fruits were 76.34±2.61; 67.52±2.45; 71.23±2.37; 75.59±3.24; 162.58±15.08; 63.70±2.67 μg/mL, respectively. Cell cycle arrest for ethylacetate extract of heartwoods and fruits were accumulated in G0/G1 phase (56.70% and 65.56%).
Conclusion: These results suggest that L. cubeba heartwoods and fruits has cell cycle arrest activity.
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References
WHO. World Cancer Report 2014. Geneva: WHO; 2015.
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin 2015;65(1):5-29.
Trisonthi P, Sato A, Nishiwaki H, Tamura H. A new diterpene from Litsea cubeba fruits: Structure elucidation and capability to induce apoptosis in HeLa cells. Molecules 2014;19(9):6838-50.
Piyapat T, Kana M, Hirotoshi T. Induction of apoptosis in hela cells by methanolic extract of Litsea cubeba fruit residue from essential oil extraction. J Life Sci 2013;7:928-34.
Feng T, Zhang RT, Tan QG, Zhang XY, Liu YP, Cai XH, et al. Two new isoquinoline alkaloids from Litsea cubeba. z. Naturforsch 2009;64:871-4.
Dalimunthe A, Achmad S, Satria D. Phenolic, flavonoid content and antioxidant activities of ethylacetate extract of Litsea cubeba (lour.) pers. barks. Der Pharm Chem 2016;8:466-8.
Satria D, Furqan M, Hadisahputra S, Rosidah. Combinational effects of ethylacetate extract of picria fel-terrae lour and doxorubicin on t47d breast cancer cells. Int J Pharm Pharm Sci 2015;7:73-6.
Hasibuan PA, Jessy C, Satria D. Combination effect of ethylacetate extracts of Plectranthus ambonicius (lour.) spreng. with doxorubicin againts t47d breast cancer cells. Int J Pharm Pharm Sci 2015;7:155-9.
Sihotang YM, Silalahi J, Hadisahputra S, Hasibuan PA, Satria D. Cardioprotective effect of ethylacetate extract of poguntano (Picria fel-terrae lour.) against doxorubicin-induced cardiotoxicity in rats. Int J Pharm Clin Res 2016;8:466-70.
Meiyanto E, Hermawan A, Junedi S, Fitriasari A, Susidarti RA. Nobilatin increased cytotoxic activity of doxorubicin on mcf-7 cells but not on t47d cells. Int J Phytomed 2011;3:129-37.
Nugroho AE, Ikawati M, Hermawan A, Putri DD, Meiyanto E. Cytotoxic effect of ethanolic extract fractions of Indonesia plant Ficus septica Burm. F. on human breast cancer t47d cell lines. Int J Phytomed 2011;3:216-26.
Nurrochmad A, Lukitaningsih E, Meiyanto E. Anti cancer activity of rodent tuber (Thyphonium flagelliforme (lodd.) blume on human breast cancer t47d cells. Int J Phytomed 2011;3:138-46.
Nugroho AE, Hermawan A, Putri DD, Novika A, Meiyanto E, Kawaichi M. Combinational effects of hexane insoluble fraction of Ficus septica Burm. F. and doxorubicin chemotherapy on T47D breast cancer cells. Asian Pac J Trop Biomed 2013;3(4):297-302.
Satria D, Nasution NP, Ilyas S. Cytotoxcicity effect of sea horse (Hippocampus trimaculatus leach.) extract and fractions on mcf-7 cell line. Int J Pharm Technol Res 2014;6:212.
Anggraini R, Hadisahputra S, Silalahi J. Combinational effects of ethylacetate extract of Zanthoxylum acanthopodium Dc. with doxorubicin on t47d breast cancer cells. Int J Pharm Technol Res 2014;6(7):2032-5.
Satria D, Silalahi J, Haro G, Ilyas S, Hsb PA. Antioxidant and antiproliferative activities of an ethylacetate fraction of picria fel-terrae lour. Herbs Asian Pac J Cancer Prev 2017;18(2):399-403.
Yadav VR, Sahdeo P, Bokyung S, Ramaswamy K, Bharat BA. Targetting inflammatory pathways by triterpenoids for prevention and treatment of cancer. Toxins 2012;2:2428-66.
Di Leo A, Tanner M, Desmedt C, Paesmans M, Cardoso F, Durbecq V, et al. p-53 gene mutations as a predictive marker in a population of advanced breast cancer patients randomly treated with doxorubicin or docetaxel in the context of a phase III clinical trial. Ann Oncol 2007;18(6):997-1003.
Vayssade M, Haddada H, Faridoni-Laurens L, Tourpin S, Valent A, Bénard J, et al. P73 functionally replaces p53 in adriamycin-treated, p53-deficient breast cancer cells. Int J Cancer 2005;116(6):860-9.
Choi HJ, Lim DY, Park JH. Induction of g1 and g2/m cell cycle arrests by the dietary compound 3,3’-diindolylmethane in ht-29 human colon cancer cells. BMC Gastroenterol 2009;9:39.
Park WH. Anti-apoptotic effect of caspase inhibitors on H2O2-treated HeLa cells through early suppression of its oxidative stress. Oncol Rep 2014;31(5):2413-21.
Yu Jh, Liu CY, Zheng GB, Zhang LY, Yan MH, Zhang WY, et al. Pseudolaric acid B induced cell cycle arrest, autophagy and senescence in murine fibrosarcoma l929 cell. Int J Med Sci 2013;10(6):707-18.
Li L, Da HJ, Mao Y, Wang SL, Xiao XJ. Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition. Cancer Cell Int 2012;12:2-6.
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