• Ashwathanarayana R Department of PG Studies and Research in Applied Botany, Kuvempu University, Jnanasahyadri, Shankaraghatta, Shimoga - 577 451, Karnataka, India.
  • Raja Naika Department of PG Studies and Research in Applied Botany, Kuvempu University, Jnanasahyadri, Shankaraghatta, Shimoga - 577 451, Karnataka, India.



Olea dioica Roxb, Benzeneethanol, 4-hydroxy-, Aphrodisiac activity


Objectives: We aimed to assess the effects of O. dioica Roxb. leaf and bark extract and its isolated constituent benzeneethanol, 4-hydroxy- on aphrodisiac activity in rats using different standard aphrodisiac parameters.

Methods: To determine the aphrodisiac activity several parameters were observed. These include measuring and observing the mount frequency (MF), mount latency (ML), intromission frequency (IF), intromission latency (IL), anogenital sniffing (AS), and genital grooming (GG).

Results: The aphrodisiac activity of O. dioica Roxb. leaf and bark extracts and its pure compound were studied on Wistar albino rats at different dosages. The parameters observed during the study were MF, ML, IF, IL, AS, and GG. The results revealed that the crude extracts showed dose-dependent activity in all the concentration, in that, ethanolic leaf extract showed excellent activity compared to ethanolic bark extract and pure compound benzene ethanol 4-hydroxy.

Conclusion: Results showed the excellent aphrodisiac activity of O. dioica Roxb. leaf and bark ethanolic extracts. However, the major compound benzeneethanol, 4-hydroxy- was not responsible for the aphrodisiac activity, may be the minor compounds even though in minor concentration in the extract influence the sexual activity in tested animals.


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Author Biography

Ashwathanarayana R, Department of PG Studies and Research in Applied Botany, Kuvempu University, Jnanasahyadri, Shankaraghatta, Shimoga - 577 451, Karnataka, India.

Research Student Department of PG studies and Reserach in Applied Botany


Elferink JG. Aphrodisiac use in pre-columbian Aztec and Inca cultures. J Hist Sex 2000;9(1-2):25-36.

Melnyk JP, Marcone MF. Aphrodisiacs from plant and animal sources - A review of current scientific literature. Food Res Int 2011;44:840-50.

Blackwell B. Antidepressant drugs. In: Dukes MN Meyer’s Side Effects of Drugs. Amsterdam: Elsevier; 1984. p. 24-61.

Kumar S, Kumar JV, Selvaraj R. Aphrodisiac activity of Cycas circinalis L and Ionidium suffruticosum Ging on male wister albino rats. Asian J Pharm Clin Res 2013;6(3):215-7.

Shekar S, Vazir M, Kempanna B, Miryala SK, Subedi A. Effect of yohimbine on clomipramine-induced sexual dysfunction in male rats. Asian J Pharm Clin Res 2017;10(2):92-6.

Pascal JP. Wet Evergreen Forests of the Western Ghats of India. Vol. 11. France: Institut Francais De Pondicherry; 1988. p. 309.

Kholkute SD. Coordinating Unit of Survey of Medicinal Plants of Western Ghats of India. A Project Report Submitted. New Delhi: Indian Council of Medical Research; 2008. p. 16-7.

Pullaiah T. Biodiversity in India. Vol. 4. New Delhi: Regency Publications; 2006. p. 281-2.

Yesodharan K, Sujana KA. Ethnomedicinal knowledge among Malamalasar tribe of Parambikulam wildlife sanctuary, Kerala. Indian J Tradit Knowl 2007;6(3):481-5.

Ashwathanarayana R, Naika R. Comparative study of different solvent extract of Olea dioica Roxb. Western Ghats, Karnataka, against selected plant and animal pathogenic bacteria. J Pharm Bio Res 2015;3(1):217-22.

Ashwathanarayana R, Naika R. Preliminary phytochemical and antimicrobial properties of Olea dioica Roxb. Bark extract collected from the Western Ghats, Karnataka, India. J Pharmacogn Phytochem 2015;4(4):156-60.

Ashwathanarayana R, Naika R. Study on antioxidant and cytotoxic properties of Olea dioica Roxb. crude extract and its pure compound collected from Western Ghats, Karnataka, India. Asian J Pharm Clin Res 2017;10(2):356-67.

Rao GS, Padmalatha HV, Udupa ES. Diversity of tree species in Sharavathi wildlife sanctuary, Shivamogga. Int J Curr Res 2016;8(8):36809-12.

De-Castro MD, Ayuso LE. Soxhlet extraction of solid materials: An outdated technique with a promising innovative future. Anal Chim Acta 1998;369:1-10.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. New York: Chapman and Hall Co.; 1998. p. 1-302.

Gartlan JS, McKey DB, Waterman PG, Mbi CN, Strusaker TT. A comparative study of the phytochemistry of two African rain forests. Biochem Syst Ecol 1980;8:401-22.

Singh G, Mukherjee T. Herbal aphrodisiacs. Indian Drugs 1998;35(4):175-82.

Tripathi KD. Essentials of Medical Pharmacology. 4th ed. New Delhi: Jaypee Brothers; 1999. p. 298-325.

Subramoniam A, Madhavachandran V, Rajasekharan S, Pushpangadan P. Aphrodisiac property of Trichopus zeylanicus extract in male mice. J Ethnopharmacol 1997;57(1):21-7.

Kothari CR. Research Methodology. 2nd ed. New Delhi: New Age International Publishers; 2004. p. 256-61.

Wani JA, Achur RN, Nema KR. Phytochemical screening and aphrodisiac activity of Asparagus racemosus. Int J Pharm Sci Drug Res 2011;3(2):112-5.

Adeneye AA, Ajagbonna OP, Adeleke TI, Bello SO. Preliminary toxicity and phytochemical studies of the stem bark aqueous extract of Musanga cecropioides in rats. J Ethnopharmacol 2006;105(3):374-9.

Panten U, Ishida H, Beckmann J. Effects of D-glyceraldehyde and 3-o-methylglucose upon fluorescence of reduced pyridine nucleotides from perifused isolated pancreatic islets. Horm Res 1976;7(3):164-71.

Pubchem-2(5H)-Furanone (C4H4O2). Available from:

Lucchini JJ, Corre J, Cremieux A. Antibacterial activity of phenolic compounds and aromatic alcohols. Res Microbiol 1990;141(4):499-510.

Zilz TR, Griffiths HR, Coleman MD. Apoptotic and necrotic effects of hexanedione derivatives on the human neuroblastoma line SK-N-SH. Toxicology 2007;231(2-3):210-4.

Jadhav V, Kalase V, Patil P. GC-MS analysis of bioactive compounds in methanolic extract of Holigarna grahamii (wight) Kurz. Int J Herb Med 2014;2(4):35-9.

PubChem-2-Furancarboxylic Acid. Available from:

Senthamarai SV, Basker A. Phytochemical analysis and GC-MS profiling in the leaves of Sauropus androgynus (l) MERR. Int J Drug Dev Res 2012;4(1):162-7.

Corre J, Lucchini JJ, Mercier GM, Cremieux A. Antibacterial activity of phenethyl alcohol and resulting membrane alterations. Res Microbiol 1990;141(4):483-97.

Moorthy V, Boominathan M. Comparative antimicrobial activities of Morus alba crude extract and fraction against Staphylococcus aureus. Int J Inst Pharm Life Sci 2011;1(2):48-56.

Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res 2010;4(7):191-5.

PubChem -4H-pyran-4-one,3,5-dihydroxy-2-methyl. Available from:

The Government of Canada, Environment, and Climate Change Canada, 1,2-benzenediol (catechol), screening assessment for 1,2-benzenediol (120-80-9), July; 2008. Available from:

de Castro Oliveira LG, Brito LM, de Moraes Alves MM, Amorim LV, Sobrinho-Júnior EP, de Carvalho CE, et al. In vitro effects of the neolignan 2,3-dihydrobenzofuran against leishmania amazonensis. Basic Clin Pharmacol Toxicol 2017;120(1):52-8.

Diaz P, Phatak SS, Xu J, Fronczek FR, Astruc-Diaz F, Thompson CM, et al. 2,3-dihydro-1-benzofuran derivatives as a series of potent selective cannabinoid receptor 2 agonists: Design, synthesis, and binding mode prediction through ligand-steered modeling. ChemMedChem 2009;4(10):1615-29.

Encyclopedia - The Free Dictionary. Available from:,2,3-propanetriol.

PubChem - 2-Methoxy-4-vinylphenolC9H10O2. Available from:

Subashini S, Rameshkannan V, Mani P. Phytochemical and GC-MS analysis of bioactive compounds from Borassus flabellifer Linn root. Eur J Mol Biol Biochem 2015;2(3):148-52.

Garzon-Aburbeh A, Jacques HP, Claesen M, Dumont P. A lymphotropic prodrug of L-Dopa: Synthesis, pharmacological properties, and pharmacokinetic behavior of 1,3-dihexadecanoyl-2-[(S)-2-amino-3-(3,4-dihydroxy phenyl) propanoyl]propane-l,2,3-triol. J Med Chem 1986;29(5):687-91.

Dey P, Dutta S, Chaudhuri TK. Comparative phytochemical profiling of Clerodendrum infortunatum L. Using GC-MS method coupled with multivariate statistical approaches. Metabolomics 2015;5:3.

PubChem - Phytol|C20H40O. Available from:

PubChem - Roseoxide/2H-Pyran,tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-|C10H18O. Available from:

PubChem - 3,4-Dimethylaniline|C8H11N. Available from:

PubChem - Methyl Isobutyrate-C5H10O2. Available from:

PubChem - 3 Ethanol, 2,2’-Oxybis C4H10O3. Available from:

Yu X, Zhao M, Liu F, Zeng S, Hu J. Identification of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one as a strong antioxidant in glucose-histidine Maillard reaction products. Food Res Int 2013;51(1):397-403.

PubChem - 5-hydroxymethylfurfural|C6H6O3. Available from:

Wikipedia - Hydroxymethylfurfural. Available from:

PubChem - 1,2,3-propanetriol, monoacetate.

Fukai S, Tanimoto S, Maeda A, Fukuda H, Okada Y, Nomura M. Pharmacological activity of compounds extracted from persimmon peel (Diospyros kaki THUNB.). J Oleo Sci 2009;58(4):213-9.

PubChem - Cis-2,6-dimethylmorpholine. Available from:

Regan J, Capolino A, Cirillo PF, Gilmore T, Graham AG, Hickey E, et al. Structure-activity relationships of the p38alpha MAP kinase inhibitor 1-(5-tert-butyl-2-p-tolyl-2H-pyrazol-3-yl)-3-[4-(2-morpholin-4-yl-ethoxy)naph-thalen-1-yl]urea (BIRB 796). J Med Chem 2003;46(22):4676-86.

PubChem - Phenol,2-methoxy-4-(1-propenyl). Available from:

Fernández MA, Sáenz MT, García MD. Anti-inflammatory activity in rats and mice of phenolic acids isolated from Scrophularia frutescens. J Pharm Pharmacol 1998;50(10):1183-6.

Tung YT, Wu JH, Kuo YH, Chang ST. Antioxidant activities of natural phenolic compounds from Acacia confusa bark. Bioresour Technol 2007;98(5):1120-3.

Cueva C, Moreno-Arribas MV, Martín-Alvarez PJ, Bills G, Vicente MF, Basilio A, et al. Antimicrobial activity of phenolic acids against commensal, probiotic and pathogenic bacteria. Res Microbiol 2010;161(5):372-82.

Lee HC, Jenner AM, Low CS, Lee YK. Effect of tea phenolics and their aromatic fecal bacterial metabolites on intestinal microbiota. Res Microbiol 2006;157(9):876-84.

PubChem - Ethanone,1-(4-hydroxy-3-methoxyphenyl). Available from:

PubChem - 1,3-benzenediol,4-propyl. Available from:

PubChem - 3,4-epoxycyclohexyl,CarboxylicAcid,3,4-Epoxycyclohexy l. Available from: /2386-87-0.

Flamini R, Traldi P. Mass Spectrometry in Grape and Wine Chemistry. UK: John Wiley and Sons; 2009. p. 232.

Gopalakrishnan S, Vadivel E. GC-MS analysis of some bioactive constituents of Mussaenda frondosa Linn. Int J Pharm Bio Sci 2011;2(1):316-9.

PubChem - 4-((1E)-3-Hydroxy-1-propenyl)-2-methoxyphenol. Available from:

Chaudhary A, Choudhary S, Sharma U, Vig AP, Arora S. In vitro evaluation of Brassica sprouts for its antioxidant and antiproliferative potential. Indian J Pharm Sci 2016;78(5):615-23.

PubChem - 1,2-dimethoxy-4-ethylbenzene. Available from:

PubChem - Benzenemethanol,3,4,5-trimethoxy. Available from:

PubChem - 2-Methoxy-4-Ethyl-6-Methylphenol. Available from:

Kelly GS. Squalene and its potential clinical uses. Altern Med Rev 1999;4(1):29-36.

PubChem - Ethanone,1-[5-[(5-methyl-2-furanyl)methyl]-2-furanyl]. Available from:

PubChem - Stigmast-5-en-3-ol,(3.beta.,24S). Available from:

Tagliamonte A, Fratta W, Gessa GL. Aphrodisiac effect of L-DOPA and apomorphine in male sexually sluggish rats. Experientia 1974;30(4):381-2.

Angrist B, Gershon S. Clinical effects of amphetamine and L-DOPA on sexuality and aggression. Compr Psychiatry 1976;17(6):715-22.

Ananthakumar KV, Srinivasan KK, Shanbhag T, Rao SG. Aphrodisiac activity of the seeds of Mucuna pruriens. Indian Drugs 1994;31:321-7.

Guohua H, Yanhua L, Rengang M, Dongzhi W, Zhengzhi M, Hua Z. Aphrodisiac properties of Allium tuberosum seeds extract. J Ethnopharmacol 2009;122(3):579-82.

Narayanswamy VB, Setty MM, Malini S, Shirwaikar A. Preliminary aphrodisiac activity of Hybanthus enneaspermus in rats. Pharmacologyonline 2007;1:152-61.

Daud D, Azahar A, Abidin SS, Tawang A. The effects of Cosmos caudatus and Piper sarmentosum aqueous extracts on male mice fertility. Int J Pharm Pharm Sci 2015;7(12):296-8.

Mensah KB, Benneh C, Boakye-Gyasi E. Ethanol root extract of the African aphrodisiac, Mondia whitie (periplocaceae), possess estrogenic activity. Int J Pharm Pharm Sci 2015;7(5):284-7.



How to Cite

R, A., and R. Naika. “STUDY ON APHRODISIAC ACTIVITY OF OLEA DIOICA ROXB. BARK, LEAF EXTRACTS, AND ITS PURE COMPOUND USING WISTAR ALBINO RATS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 12, Dec. 2017, pp. 85-98, doi:10.22159/ajpcr.2017.v10i12.21197.



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