• Raja Naika
  • Vinayaka K.S.
  • Joy Hoskeri H.


Epiprinus mallotiformis, anthelmintic, antioxidant, leaf extracts


Objective: Development of anthelmintic resistance and high cost of conventional anthelmintic drugs led to the evaluation of medicinal plants as an alternative source of anthelmintics. The aim of the present study was to investigate the anthelmintic activity and antioxidant activity of the leaves of Epiprinus mallotiformis extract using adult earthworm, Pheritima posthuma.

Methods: The leaves extracts of Epiprinus mallotiformis at different concentrations of 20 mg/mL, 40 mg/mL, 60 mg/mL, 80 mg/mL and 100 mg/mL were tested, which involve determination of paralysis time and death time.

Results: It was found that the extract exhibited significant dose dependent anthelmintic activity. Piperazine citrate 10 mg/ml as standard reference. Whereas antioxidant activity of the extracts were performed by four method, DPPH  free radical scavenging activity, H2O2 scavenging activity, Reducing power assay and Metal chelating activity using ascorbic acid as a standard. The antioxidant activity was found more in the methanol extracts show comparable activity with ascorbic acid. In the metal chelating activity the petroleum ether show maximum activity when compare with chloroform and methanol.

Conclusion: The leaves extracts of Epiprinus mallotiformis possess antioxidant and anthelminthic property. Further investigations on in vivo antioxidant activity need to be carried out to unmask its mode of action.

Keywords: Epiprinus mallotiformis; anthelmintic; antioxidant; leaf extracts.


Download data is not yet available.


Ambujakshi HR, Thakkar H, Shyamnanda. Anthelmintic activity of Gmelina arborea Roxb. leaves extract. Int J Pharm Res Develop 2009; 1(9): 1-4.

Deore SL, Khadabadi SS. In vitro antihelmintic studies of Chlorophytum borivilianum Sant. & Fernandez tubers. Indian J Nat Prod Resour 2010; 1(1): 53-56.

Goodman, Gillman. The pharmacological basis of therapeutics, 10th Ed.: Medica Publishers division; 2001. p. 1121.

Tripathi KD. Essentials of Medical Pharmacology, 5th Ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd; 2003. p. 759.

Gutteridge JM. Free radicals in disease processes: a compilation of cause and consequence. Free Radical Res 1993; 19: 141-158..

Knight JA. Diseases related to oxygen- derived free radicals. Annals of Clinical and Laboratory Sci 1995; 25(2): 111-121.

Rechner AR, Kuhnle G, Bremmer P, Hubbard GP, Moore KP, Rice-Evans CA. The metabolic fate of dietary polyphenols in humans. Free Radl Bio Med 2002; 33: 220-235.

Vinayaka KS, Praveen Kumar SV, Prashith Kekuda TR, Krishnamurthy YL, Mallikarjun N. Swathi D. Proximate composition, antioxidant, anthelmintic and insecticidal activity of a macrolichen Ramalina conduplicans vain. (ramalinaceae) European J App Sci 2009 1(3); 40-46.

Gamble JS. Flora of Presidency of Madras. Ed. 2. Bisanth Singh & Mahendra Pal Singh, Dehradon Publication; 2006. p. 1323-1324.

Balakrishna G. Vanaspathi Kosha. 1st ed., Kalpatharu Research Academy, India; 2004. p. 73.

Prashant T, Bimlesh K, Mandeep K, Gurpreet K, Harleen K. Phytochemical screening and extraction: A Review. Int Pharm Sci 2011; 1(1): 98-106.

Ajaiyeoba EO,.Onocha PA, Olarenwaju OT. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extract. Pharm Biol 2001; 39: 217- 20.

Vidyarthi RD. A Text Book of Zoology. 14th ed. S. Chand and Co, New Delhi; 1967.

Thorn GW, Adams RD, Braunwald E, Isselbacher KJ, Petersdorf RG. Harrison's Principles of Internal Medicine. New York: McGraw Hill Co; 1977.

Vigar Z. Atlas of Medical Parasitology. 2nd ed. Singapore: P.G. Publishing House; 1984.

Chatterjee KD. Parasitology, Protozoology and Helminthology. 6th ed.Calcutta: In Guha Ray Sree Saraswaty Press Ltd, India; 1967.

Bhanu P, Manoj G, Poonam J, Sarika Z. Preliminary phytochemical screening and in vitro anthelmintic activity of Kigelia africana (Bignoniaceae) Int J Chem Sci 2012; 10(4):1799-1804.

Haquerabiu, Mondal S, Ghosh P. Investigation of in vitro anthelmintic activity of Cinnamomum Camphor leaves. Int J Drug Develop Res 2011; 3(1): 295-300.

Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia terapotensis. J Nat Prod 2001; 64: 892–895.

Dinis TC, Maderia VM, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994; 315:161-169.

Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 1986; 44: 307-315.

Ruch RT, Cheng SJ, Klaunig JE. Spin trapping of superoxide and hydroxyl radicals. Meth Enzymol 1984; 105: 198-209.

Haung D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agri Chem 2005; 53: 1841-1856.

Dhully JN, Raman PH, Mujumdar AM, Naik SR. Inhibition of lipid peroxidation by piperine during experimental inflammation in rats. Indian J Exp Biol 1993; 31: 443-445.

Reddy KH, Aharma PVGK, Reddy OVS. A comparative in vitro study on antifungal and antioxidant activities of Nervilia aragoana and Atlantia monophylla. Pharm Biol 2010; 48: 595-602.

Diplock AT. Will the good fairies please prove to us that vitamin E lessens human degenerative disease? Free Rad Res 1997; 27: 511-532.

Pin-Der-Duh X. Antioxidant activity of burdock (Arctiumlappalinne): Its scavenging effect on free radical and active oxygen. J Am Oil Chem Soc 1998; 75: 455-461.

Gordon MH. The mechanism of antioxidant action in vitro. In: BJF Hudson (Ed.), Food antioxidants Elsevier Applied Science, London; 1990. p. 1–18.



How to Cite

M.B., C., R. Naika, V. K.S., and J. Hoskeri H. “EVALUATION OF ANTHELMINTIC AND ANTIOXIDANT ACTIVITIES OF EPIPRINUS MALLOTIFORMIS LEAF EXTRACTS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 8, Sept. 2013, pp. 199-02, https://journals.innovareacademics.in/index.php/ajpcr/article/view/386.