EFFECT OF DIFFERENT TYPES OF MILK FORMULA AGAINST STREPTOCOCCUS MUTANS BIOFILM FORMATION IN VITRO

Meltharyna Meltharyna; Margaretha Suharsini; Heriandi Sutadi

doi:10.22159/ajpcr.2017.v10s5.23088

Authors

Meltharyna Meltharyna Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Margaretha Suharsini Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Heriandi Sutadi Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23088

Keywords:

Milk formula, Biofilm, Streptococcus mutans

Abstract

Objectives: The purpose of this study was to analyze the differences in the effect of milk-based, soy-based, and protein hydrolysate formulas against Streptococcus mutans (SM) biofilm formation.

Methods: Infant formula used is intended for children aged 1-3 years, and each respective type of infant formula is represented by three distinct brands.

Results: Our results showed a significant difference in the effect of milk-based, soy-based, and protein hydrolysate formulas against SM biofilm formation (p=0.002).

Conclusion: Cow's milk-based formula showed the lowest SM biofilm formation.

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References

Kumar VD. Early childhood caries-an insight. J Int Oral Health 2010;2(1):1-9.

Dean JA, Avery DR, McDonald RE. Dentistry for the Child and Adolescent. Missouri: Elsevier Health Sciences; 2010.

Chaudhary SD, Chaudhary M, Singh A, Kunte S. An assessment of the cariogenicity of commonly used infant milk formulae using microbiological and biochemical methods. Int J Dent 2011;2011:1-9.

de Mazer Papa AM, Tabchoury CP, Del Bel Cury AA, Tenuta LM, Arthur RA, Cury JA. Effect of milk and soy-based infant formulas on in situ demineralization of human primary enamel. Pediatr Dent 2010;32(1):35-40.

PAHO, WHO. Guiding Principles for Complementary Feeding of the Breastfed Child. Geneva: Pan American Health Organization, World Health Organization; 2002.

Samaranayake LP, Jones BM. Essential Microbiology for Dentistry. 2nd ed. London: Churchill Livingstone; 2001.

McGhee JR, Michalek SM, Cassell GH. Dental Microbiology. 4th ed. Philadelphia, PA: Harper & Row; 1982.

Bowden GH, Li YH. Nutritional influences on biofilm development. Adv Dent Res 1997;11(1):81-99.

Erickson PR, McClintock KL, Green N, LaFleur J. Estimation of the caries-related risk associated with infant formulas. Pediatr Dent 1998;20(7):395-403.

Palmer EA, Nielsen T, Peirano P, Nguyen AT, Vo A, Nguyen A, et al. Children with severe early childhood caries: Pilot study examining Mutans streptococci genotypic strains after full-mouth caries restorative therapy. Pediatr Dent 2012;34(2):1-10.

Fauziah E. Prediksi resiko sindroma karies botol berdasarkan polimorfisme gen glucosyltransferase Streptococcus mutans dan level mucin MG2 saliva. Jakarta: Universitas Indonesia; 2013.

Wan AK, Seow WK, Walsh LJ, Bird PS. Comparison of five selective media for the growth and enumeration of Streptococcus mutans. Aust Dent J 2002;47(1):21-6.

Mehrotra RS. Principles of Microbiology. New Delhi: McGraw-Hill Education Pvt Limited; 2009.

Marsh PD, Martin MV. Oral Microbiology. 4th ed. Oxford, UK: Elsevier Health Sciences; 1999.

Allison LM, Walker LA, Sanders BJ, Yang Z, Eckert G, Gregory RL. Effect of human milk and its components on Streptococcus mutans biofilm formation. J Clin Pediatr Dent 2015;39(3):255-61.

Nassar HM, Li M, Gregory RL. Effect of honey on Streptococcus mutans growth and biofilm formation. Appl Environ Microbiol 2012;78(2):536-40.

Merritt JH, Kadouri DE, Oâ€™Toole GA. Growing and analyzing static biofilms. Curr Protoc Microbiol 2005;Chapter 1:Unit 1B.1.

Coffey BM, Anderson GG. Biofilm formation in the 96-well microtiter plate. Methods Mol Biol 2014;1149:631-41.

Gronroos L. Quantitative and Qualitative Characterization of Mutans streptococci in Saliva and in the Dentition. Helsinki: University of Helsinki; 2000.

Seneviratne CJ, Zhang CF, Samaranayake LP. Dental plaque biofilm in oral health and disease. Chin J Dent Res 2011;14(2):87-94.

Johansson I. Milk and dairy products: Possible effects on dental health. Food Nutr Res 2002;46(3):119-22.

Mount GJ, Hume WR. Preservation and Restoration of Tooth Structure. 2nd ed. Queensland: Knowledge Books and Software; 2005.

Colak H, DÃ¼lgergil CT, Dalli M, Hamidi MM. Early childhood caries update: A review of causes, diagnoses, and treatments. J Nat Sci Biol Med 2013;4(1):29-38.

Law V, Seow WK, Townsend G. Factors influencing oral colonization of mutans streptococci in young children. Aust Dent J 2007;52(2):93 100.

Chandki R, Banthia P, Banthia R. Biofilms: A microbial home. J Indian Soc Periodontol 2011;15(2):111-4.

Kim YS, Lee ES, Kwon HK, Kim BI. Monitoring the maturation process of a dental microcosm biofilm using the Quantitative Light-induced Fluorescence-Digital (QLF-D). J Dent 2014;42(6):691-6.

Vacca-Smith AM, Van Wuyckhuyse BC, Tabak LA, Bowen WH. The effect of milk and casein proteins on the adherence of Streptococcus mutans to saliva-coated hydroxyapatite. Arch Oral Biol 1994;39(12):1063-9.

Assaf D, Steinberg D, Shemesh M. Lactose triggers biofilm formation by Streptococcus mutans. Int Dairy J 2015;42:51-7.

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