ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF ASAM KANDIS (GARCINIA XANTHOCHYMUS HOOK. F. EX T. ANDERSON) FRUIT
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i4.23578Keywords:
Asam kandis, Anti-inflammatory, Carrageenan, Paw edema, Cotton pelletAbstract
 Objective: The objective of this study was to evaluate the anti-inflammatory activity in acute and subacute models of inflammation from ethanolic fruit extract of Asam kandis (Garcinia xanthochymus Hook. f. ex T. Anderson) in animal (rats) models.
Methods: Pleliminary phytochemical screening was carried out by using standard procedures.. Assessment of acute and subacute models of inflammation was using carrageenan-induced paw edema method and cotton pellet granuloma method using three dosage treatments; 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW along with a negative control group (0.5% Na CMC) and positive control (Na diclofenac 2.25 mg/kg BW). The inhibition period was observed at 30, 60, 90, 120, 150, and 180 min time intervals.
Result: The phytochemical screening showed that the ethanolic fruit extract from Asam kandis contain contains flavonoids, glycosides, steroids, and triterpenoids. The anti-inflammatory result showed that the strongest inhibition produced by ethanolic fruit extract of Asam kandis occurred on the dosage of 800 mg/kg BW compared to the other doses (200 and 400 mg/kg BW) throughout the observation period.
Conclusion: This finding indicated that ethanolic fruit extract of Asam kandis (G. xanthochymus Hook. f. ex T. Anderson) might become an interesting candidate for treatment of inflammation.
Downloads
References
Bhatia DK, Paliwal SK. Membrane stabilzation, inhibition of histamine and prostaglandin synthesis mediated anti-inflammatory response of some indigenous plants. Int J Pharm 2014;1:660-7.
Lee CJ, Chen LG, Liang WL, Wang CC. Antiinflammatory effects of Punica granatum Linne in vitro and in vivo. Food Chem 2010;118:315 22.
Rim HK, Yun CH, Shin JS, Cho YW, Jang DS, Ryu JH, et al. 5,6,7-trimethoxyflavone suppresses pro-inflammatory mediators in lipopoly-saccharide-induced RAW 264.7macrophages and protects mice from lethal endotoxinshock. Food Chem Toxico 2013;62:847-55.
Sutrisno E, Adnyana K, Sukandar EY, Fidrianny I, Aligita W. Anti-inflammaotry study of Anredera cordifolia leaves and Centella asiatica herbs and its combination using human red blood cell-membrane stabilization method. Asian J Pharm Clin Res 2016;9:78-80.
Joyce KL, Hayes ER. Farmakologi Pendekatan Proses Keperawatan. Jakarta: Penerbit Buku Kedokteran ECG; 1996.
Wallace JL, Vong L. NSAID-induced gastrointestinal damage and the design of GI-sparing NSAIDs. Curr Opin Investig Drugs 2008;9:1151 6.
Cragg GM, Newman, DJ. Natural products: A continuing source of novel drug leads. Biochim Biophys Acta 2013;1830:3670-95.
Ibrahim B, Sowemimo A, van Rooyen A, Van de Venter M. Anti inflammatory, analgesic and antioxidant activities of Cyathula prostrata (Linn.) Blume (Amaranthaceae). J Ethnopharmacol 2012;141:282-9.
Kumar S, Sharma S, Chattopadhyay SK. The potential health benefit of polyisoprenylated benzophenones from Garcinia and related genera: Ethnobotanical and therapeutic importance. Fitoterapia 2013;89:86 125.
Winata HS. Antioxidant and Anti-Inflammatory Activity of Asam Kandis Fruit (Garcinia xanthocymus) Ethanolum Extract Against White Male Rats. Thesis Faculty of Pharmacy, University of Sumatera Utara; 2017. p. 1-104.
Ditjen PO. Farmakope Indonesia. Depkes RI 1995;4:896-8.
Depkes RI. Materia Medika. Ditjen POM 1995;6:297-307.
Farnsworth NR. Biologycal and phytochemical screening of plants. J Pharm Sci 1996;55:225-76.
Harbone JB. Metode Fitokimia. ITB 1987;2:6, 49.
Winter CA, Risley EA, Nuss GW. Carrageenin induced oedema in hindpaws of the rats as an assay of anti-inflammatory drugs. Proc Soc ExpBiol Med 1962;111:544-7.
Yu D, Yuan Y, Jiang L, Tai Y, Yang X, Hu F, et al. Anti-inflammatory effects of essential oil in Echinacea purpurea L. Pak. J Pharm Sci 2013;26:403-8.
Swingle KF, Shiderman FE. Phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modificationby certain anti-inflammatory agents. J Pharm Exp Ther 1972;183:226-34.
Ramadhani IM, Lestari F, Yuniarni U. Pengaruh Ekstrak Etanol Kulit Buah Asam Kandis (Garcinia parvifolia) Seabgai Hepatoprotektro Pada Tikus Wistar Jantan Yang Diiduksi Isoniazid dan Rifampisin. Prosiding Penelitian SPeSIA; 2015.
Haro G, Sinaga SM, Iksen I, Nerdy N, Theerachetmongkol S. Protective effect of chives leaves (Allioum schoenoprasum, L.) infusion against ethylene glycol and ammonium chloride induced nephrolithiasis in rats. J Appl Pharm Sci 2017;7:222-5.
Shin JS, Baek SR, Sohn SI, Cho YW, Lee KT. Anti-inflammatory effect of pelubiprofen acid, mediated by dual supression of COX activity and LPS-induced inflammatory gene expression via NF-kB inactivation. J Cell Biochem 2011;112:3594-603.
Yun KJ, Koh DJ, Kim SH, Park SJ, Ru SJ, Kim DG, et al. Anti inflammatory effects of sinapic acid through the suppression od inducible nitric acid oxide synthase, cycooxygase-2 and proinflmammatory cytokinesis expressions via nuclear factor-kappaB inactivation. J Agric Food Chem 2008;56:10265-72.
Martini ND, Katerere DR, Eloff JN. Biological activity of five antibacterial flavonoids from Combretum erythrophyllum (Combretaaceae). J Ethnopharmacol 2004;93:207-12.
Nguyen VL, Truong CT, Nguyen BC, Vo TV, Dao TT, Nguyen VD, et al. Anti-inflammatory and wound healing activities of calophyllolide isolated from Calophyllum inophyllum Linn. Plos One 2017;12:1-16.
Toker G, Kupelo E, Memisoolu M, Yesilada E. Flavonoids with antinociceptive and antiinflammatory activities from the leaves of Tilia argentea (Silver Linden). J Ethnopharmacol 2004;95:393-7.
Mehta V, Sharma A, Kailkhura P, Malairaman U. Antioxidant, anti0inflammatory and antidiabetic activity of hydroalcoholic extract of Ocimum sanctum: An in vitro and in dilico study. Asian J Pharm Clin Res 2016;9:44-9.
Vinueza D, Lopez E, Acosta K, Abdo S. Assessment of anti-inflammaotry activity and cytotoxicity of freeze dried hydroalcoholic extract of Bidens andicola on isolated neutrophils. Asian J Pharm Clin Res 2017;10:160-3.
Ullah AH, Akter L, Zaman S, Juhara F, Tareq SM, Bhattacharjee R, et al. Anti-inflmammatory and analgesic activities of methanolic seed extract of Sterculia villosa Roxb. Asian J Pharm Clin Res 2015;8:247 51.
Laaboudi W, Ghanam J, Aissam H, Merzouki M, Benlemlih M. Anti-inflammatory and analgesic activities of olive tree extract. Int J Pharm Pharm Sci 2016;8:414-9.
Shaikh S, Joshi YM, Kadam V. Comparative study of anti-inflammatory activity if aqueous and methanolic extracts of Hibiscus cannabinus leaf (Malvaceae). Int J Pharm Pharm Sci 2016;8:64-8.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
