HEPATOPROTECTIVE ACTIVITY OF VERNONIA AMYGDALINA LEAF ETHANOLIC EXTRACT IN WHITE RATS INDUCED BY PARACETAMOL

Panal Sitorus; Nerdy Nerdy

doi:10.22159/ajpcr.2018.v11i10.29881

Authors

Panal Sitorus Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan Selayang, Medan Baru, Sumatera Utara, Indonesia.
Nerdy Nerdy Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan Selayang, Medan Baru, Sumatera Utara, Indonesia. http://orcid.org/0000-0002-1993-4415

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i10.29881

Keywords:

Hepatoprotective Activity, Vernonia amygdalina, Ethanolic Extract, White Rats, Paracetamol

Abstract

Objectives: A study on the hepatoprotective effect of Vernonia amygdalina leaves ethanolic extract on white rats induced by paracetamol was aimed to obtaining scientific data and evidence the hepatoprotective ability of the extract.

Methods: The research was a complete randomized design, using 36 white rats which were divided into 6 groups. The normal control group was given 1% carboxy methyl cellulose for 7 days and followed by distilled water 8 hours after treatment. The positive control group was given 100 mg curcumin per kg body weight of rats for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. The negative control group was given 1% carboxy methyl cellulose for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. The test group was given 50, 100 and 200 mg extract per kg body weight of rats for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. Blood sampling was carried out 3 times (before treatment, after treatment and after induction).

Results: With doses of 50, 100, and 200 mg extract per kg body weight of rats give significant difference on the decrease of Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels after paracetamol administration.

Conclusions: It can be concluded that Vernonia amygdalina leaves ethanolic extract were potentially hepatoprotective.

Downloads

Download data is not yet available.

References

Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules 2016;21:1-18.

Verma R. A review on hepatoprotective activity of medicinal plants. J Med Plants Stud 2018;6:188-90.

Huang ZQ, Chen P, Su WW, Wang YG, Wu H, Peng W, et al. Antioxidant activity and hepatoprotective potential of quercetin 7-rhamnoside in vitro and in vivo. Molecules 2018;23:1-13.

Ginting N, Suwarso E, Rumapea DV, Nerdy N. Relaxation activity of tetanus (Leea aequata L.) leaf ethanolic extract on Guinea pig isolated trachea. Asian J Pharm Clin Res 2018;11:1-4.

Haro G, Sinaga SM, Iksen I, Nerdy N, Theerachetmongkol S. Protective effect of chives leaves (Allioum schoenoprasum, L.) infusion against ethylene glycol and ammonium chloride induced nephrolithiasis in rats. J App Pharm Sci 2017;7:222-5.

Putra ED, Ginting N, Nazliniwaty N, Iksen I, Kurniawan E, Nerdy N. In vitro antinephrolithiasis effect of breadfruit (Artocarpus altilis (park) fosberg) leaves extract by atomic absorption spectrophotometry. Asian J Pharm Clin Res 2018;11:1-4.

Karsono K, Patilaya P, Azisah N, Nerdy N. Comparison of antimicrobial activity of red betel (Piper crocatum Ruiz and Pav) leaves nanoparticle and powder ethanolic extract against methicillin resistant Staphylococcus aureus. Int J PharmTech Res 2015;8:696-701.

Masfria M, Haro G, Nerdy N, Mierza V, Wahyuni HS, Permata YM. Antimicrobials activity, antioxidants activity and analysis of active extract chemical compounds content of moringa (Moringa oleifera) leaf. Int J PharmTech Res 2016;9:110-20.

Nerdy N, Manurung K. Spectrophotometric method for antioxidant activity test and total phenolic determination of red dragon fruit leaves and white dragon fruit leaves. Rasayan J Chem 2018;11:1183-94.

Nazliniwaty N, Karsono K, Zebua NF, Nerdy N. Ethanol extract grapefruit peel (Citrus maxima Murr.) gel formulations with gelling agent durian seed gum and carboxy methyl cellulose. Pharm Chem 2016;8:207-13.

Nazliniwaty N, Karsono K, Zebua NF, Nerdy N. Antioxidant activity and antiaging gel formulation grapefruit peel (Citrus maxima Merr.) ethanolic extract. Pharm Lett 2016;8:84-94.

Nerdy N. In silico docking of chemical compounds from roselle calyces (Hibiscus sabdariffa L.) as antidiabetic. Int J ChemTech Res 2015;7:148-52.

Nerdy N. In silico docking roselle (Hibiscus sabdariffa L.) calyces flavonoids as antimalarial against plasmepsin 1 and plasmepsin 2. Asian J Pharm Clin Res 2017;10:183-6.

Nerdy N. In silico study of sesquiterpene lactone compounds from South Africa leaves (Vernonia amygdalina Del.) as antimalarial and anticancer. Int J PharmTech Res 2015;7:47-53.

Nerdy N, Putra ED, Haro G, Harahap U, Hutagaol R, Karsono K. In silico screening of hesperetin and naringenin ester derivatives as anticancer against p-glycoprotein. Int J Pharm Pharm Sci 2015;7:485-8.

Nerdy N, Putra ED, Haro G, Harahap U. In silico screening of hesperetin and naringenin ester derivatives as anticancer against phosphoinositide 3-kinase. Int J PharmTech Res 2016;9:388-94.

Tarigan MH, Harahap U, Dalimunthe A, Nerdy N. Antioxidant activity and cardioprotective activity of bangun-bangun leaves (plectranthus amboinicus lour.) ethanolic extract. Asian J Pharm Clin Res 2018;11:165-8.

Vasanthkumar T, Hanumanthappa M, Prabhakar BT, Hanumanthappa SK. Hepatoprotective effect of curcumin and capsaicin against lipopolysaccharide induced liver damage in mice. Pharmacog J 2017;9:947-51.

Alghazeer R, Elgahmasi S, Elnfati AH, Elhensheri M, Al-Griw MA, Awayn N, et al. Antioxidant activity and hepatoprotective potential of flavonoids from Arbutus pavarii against CCl4 induced hepatic damage. Biotech J Int 2018;21:1-12.

Manohar VR, Pai MR, Sabitha P, Paipushpalatha P, Rai M, Ullal SD. Hepatoprotective activity of Phyllanthus niruri in thioacetamide induced hepatotoxicity in male wistar rats. Int J Pharm Pharm Sci 2014;6:341-3.

Ramachandran A, Jaeschke H. Mechanisms of acetaminophen hepatotoxicity and their translation to the human pathophysiology. J Clin Transl Res 2017;3 S1:157-69.

Chiu YJ, Chou SC, Chiu CS, Kao CP, Wu KC, Chen CJ, et al. Hepatoprotective effect of the ethanol extract of Polygonum orientale on carbon tetrachloride induced acute liver injury in mice. J Food Drug Anal 2018;26:369-79.

Nchouwet ML, Ngnokam SL, Kodjio N, Poualeu SK, Nkengeffouet PA, Kamanyi A. Hepatoprotective and antioxidant effect of stem barks extracts methanolic and aqueous of Pseudocedrela kotschyi (Meliaceae) on paracetamol induced hepatic damage in rats. Asian J Biomed Pharm Sci 2017;7:1-9.

Ye H, Nelson LJ, del Moral MG, MartÃnez-Naves E, Cubero FJ. Dissecting the molecular pathophysiology of drug induced liver injury. World J Gastroenterol 2018;24:1373-85.