RESTORATIVE EFFECTS OF ZINGIBER OFFICINALE ON ARSENIC-INDUCED GENOTOXIC EFFECTS IN CHROMOSOMAL STRUCTURE OF MICE
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i7.26505Keywords:
Arsenic (As), Chromosomes, Zingiber officinale, GenotoxicityAbstract
Objective: The present study investigated the antigenotoxic effect of Zingiber officinale (Ginger) on arsenic-induced genotoxic effects in chromosome structure of Swiss albino mice.
Methods: Albino mice were divided into four groups: Group I was kept as control, Group II was administrated an oral dose of arsenic trioxide 5 mg/kg bw for 15 days, Group III was given an oral dose of arsenic trioxide 5 mg/kg bw followed by 20 mg/kg bw of ginger orally, and Group IV was administered with 20 mg/kg bw of ginger orally for 15 days and kept as positive control. The autopsies were done from all the groups on 15-day post-treatment, and bone marrow was removed for chromosomal study and for calculating the frequency of micronuclei (MN).
Results: It is concluded from this study that long-term arsenic exposure causes chromosomal anomalies, for example, breaks, gaps, rings, stretching, and stickiness which leads to the fragmentation of chromosomes and increases the frequency of MN.
Conclusion: The administration of ginger showed modulatory effects against arsenic-induced genotoxicity.
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Chowdhary R, Dutta A, Chaudhuri S, Sharma N, Giri A, Chaudhuri K. In vitro and in vivo reduction of sodium arsenite induced toxicity by aqueous garlic extract. Food Chem Toxicol 2008;19:105-12.
Alarifi SA, Alkahtani FM, Abou-tarboush, Al-qahtani A. Effect of DNA hypomethylation on genotoxicity and apoptogenicity of sodium arsenite in laboratory mice. Pak J Biol Sci 2009;12:554-64.
Raghu KG, Cherian OL. Characterization of cytotoxicity induced by arsenic trioxide (a potent anti-APL drug) in rat cardiac myocytes. J Trace Elements Med Biol 2009;23:61-8.
Toribio M, Romanya J. Leaching of heavy metals (Cu, Ni and Zn) and organic matter after sewage sludge application to Mediterranean forest soils. Sci Total Environ 2005;363:11-21.
Patlolla AK, Todorov TI, Tchounwou PB, Voet GV, Centeno JA. Arsenic-induced biochemical and genotoxic effects and distribution in tissues of Sprague-Dawley rats. Microchem J 2012;105:101-7.
Brown EJ, Button DK. A simple method of arsenic speciation. Bull Environ Contam Toxicol 1979;21:37-42.
Tsuda T, Babazono A, Yamamoto E. Ingested arsenic and internal cancer: A historical cohort study followed for 33 years. Am J Epidemiol 1995;141:198-9.
Mazumder DN, Haque R, Ghosh N, De BK, Santra A, Chakraborti D, et al. Arsenic in drinking water and the prevalence of respiratory effects in west bengal, India Int J Epidemiol 2000;29:1047-52.
Milton AH, Hasan Z, Rahman M. Chronic arsenic poisoning and respiratory effects in Bangladesh. J Occup Health 2001;43:136-40.
Lynn S, Lai HT, Gurr JR, Jan KY. Arsenite retards DNA break rejoining by inhibiting DNA ligation. Mutagenesis 1997;12:353-58.
Lynn S, Shiung JN, Gurr JR, Jan KY. Arsenite stimulates poly (ADP ribosylation) by generation of nitric oxide. Free Radical Biol Med 1998;24:442-49.
Yamanaka K, Hayashi H, Tachikawa M, Kato K, Hasegawa A, Oku N, et al. Metabolic methylation is a possible genotoxicity-enhancing process of inorganic arsenics. Mutat Res 1997;394:95-101.
Sutar RC, Kalaichelvan VK. Evaluation of antioxidant activity of leaf extracts of Holoptelea integrifolia (roxb) Planch. Int J App Pharm 2014;6:6-8.
Hartwig A, Groblinghoff UD, Beyersmann D, Natarajan AT, Filon R, Mullenders LH, et al. Interaction of arsenic(III) with nucleotide excision repair in UV-irradiated human fibroblasts. Carcinogenesis 1997;18:399-405.
Uong JI, Luo XM. ???. Mutat Res 1993;302:97-102.
Rima T, Narasu ML. Evaluation of cytotoxic and genotoxic effects of zerumbone on colon adenocarcinoma colo205 cells and human lymphocytes. Int J Pharm Pharm Sci 2017;9:92-6.
Saravanan V, Murugan SS, Rajkumar JS, Krishnan KR. Evaluation of genotoxic effects of alcoholic extract of Kalanchoe pinnata Using Single Cell Gel Electrophoresis. Asian J Pharm Clin Res 2018;11:90-3.
Lampe JW. Health effects of vegetables and fruit: Assessing mechanisms of action inhuman experimental studies. Am J Clin Nutr 1999;70:475-90.
Wang WH, Wang ZM. Studies of commonly used traditional medicine ginger. Zhongguo Zhong Yao Za Zhi 2005;20:1569-73.
White B. Ginger: An overview. Am Fam Phys 2007;75:1689-91.
Shukla Y, Madhulika S. Cancer preventive properties of ginger: A brief review. Food Chem Toxicol 2007;45:683-90.
Altman RD, Marcussen KC. Effects of a ginger extract on knee pain in patients with osteoarthritis. Arthritis Rheum 2001;44:2531-38.
Surh YJ. Molecular mechanisms of chemo preventive effects of selected dietary and medical phenolic substances. Mutat Res 1999;428:305-27.
Shimada T. Xenobiotic-metabolizing enzymes involved in activation and detoxification of carcinogenic polycyclic aromatic hydrocarbons Drug Metab Pharmacokinet 2006;21:257-76.
Sajwicz W. Toxicity of pyrimidine derivatives under oxidative stress conditions: Chemiluminescence based assays in systems containing erythrocytes, mitochondria or blood plasma. Pharmacol Rep 2007;59:206-15.
Hussein MA. The effect of Zingiber officinalis aqueous extract on some biochemical parameters and kidney function in male mice. Kufa Med J 2012;15:273-8.
Ashraf K, Sultan S, Shah, SA. Phychemistry, phytochemical, pharmacological and molecular study of Zingiber officinale roscoe: A review. Int J Pharm Pharm Sci 2017;9:8-16.
Preston RJ, Dean BJ, Galloway S, Holden H, Mcfee AF, Shelby M. Mammalian in vivo cytogenetic assays: Analysis of chromosome aberrations in bone marrow cells. Mutat Res 1987;189:157-65.
Elbin KE, Bowen ME, Cromey DW. Arsenite and monomethylarsonous acid generate oxidative stress response in human bladder cell culture. Toxicol Appl Pharmacol 2006;217:7-14.
Ramana CV, Boldogh I, Izumi T, Mitra S. Activation of apurinic/ apyrimidinic endonuclease in human cells by reactive oxygen species and its correlation with their adaptive response to genotoxicity of free radicals. Proc Natl Acad Sci U S A 1998;95:5061-6.
Zhou Y, Lee AS. Mechanism for the suppression of the Mammalian stress response by genistein, an anticancer phytoestrogen from soy. J Natl Cancer Inst 1998;90:381-8.
Anterpreet C, Adesh KS, Anil KC, Reena VS. Natural antioxidants as defense system against cancer. Asian J Pharm Clin Res 2018;11:38-44.
Yamanaka K, Hasegawa A, Sawamura R, Okada S. Cellular response to oxidative damage in lung induced by the administration of dimethylarsinic acid, a major metabolite of inorganic arsenics, in mice. Toxicol Appl Pharmacol 1991;108:205-13.
Aflanie I, Muhyi R, Suhartono E. Effect of heavy metal on malondialdehyde and advanced oxidation protein products concentration: A focus on arsenic, cadmium, and mercury. J. Med Bioeng 2015;4:1-6.
Helleday T, Nilsson R, Jenssen D. Arsenic [III] and heavy metal ions induce intrachromosomal homologous recombination in the hprt gene V79 Chinese hamster cells. Environ Mol Mutagen 2000;35:114-22.
Nirmala K, Prasanna T, Krishna Polasa K. In vivo antimutagenic potential of Zinger on formation and excretion of mutagensin rats. Int J Canc Res 2007;3:134-42.
Kumar R, Gahlot V, Ali M, Akhtar S, Kumar A. Genotoxic effect of arsenic on chromosome structure of mice. Eur J Pharm Med Res 2016;3:224-7.
Vimala S, Norhanom AW, Yadav M. Anti-tumor promoter activity in Malaysian ginger rhizome used in traditional medicine. Br J Cancer 1999;80:110-16.
Sultana S, Ripa FA, Hamid K. Comparative antioxidant activity study of some commonly used spices in Bangladesh. Pak J Biol Sci 2010;13:340-43.
Haleagrahara N, Jackie T, Chakravarthi S, Rao M, Kulur A. Protective effect of Etlingera elatior (torch ginger) extract on lead acetate-- induced hepatotoxicity in rats. J Toxicol Sci 2010;35:663-71.
Al-Sharif MM. Effect of therapeutic ginger on genotoxic of taxol drug (Anti-cancer) in bone marrow cell of male mice. J Lif Sci 2011;5:897-905.
Abd-Allah GA, El-Bakry KA, Bahnasawy MH, El-Khodary E. Protective effects of curcumin and ginger on liver cirrhosis induced by carbon tetrachloride in rats. Int J Pharm 2016;12:361-9.
Sharif AZ. Effect of therapeutic ginger on genotoxic of taxol drug (Anti- Cancer) in bone marrow cell of male mice. J Life Sci 2011;5:897-905.
Lewinska D, Arkusz J, Stanczyk M, Palus J, Dziubaltowska E, Stepnik M. Comparison of the effects of arsenic and cadmium on benzo (a) pyrene induced micronuclei in mouse bone marrow. Mutat Res Genet Toxicol Environ Mutagen 2007;623:37-43.
Kumar SM, Unnikrishnan MK, Devi UP. Effect of 5-aminosalicyclic acid on radiation induced micronuclei in mouse bone marrow. Mutat Res 2003;527:7.
Dulout FN, Grillo CA, Seoane AI, Maderna CR, Nilsson R, Vahter M, et al. Chromosomal aberrations in peripheral blood lymphocytes from native Andean women and children from northwestern Argentina exposed to arsenic in drinking water. Mutat Res 1996;370:151-8.
Morita T, Asano N, Awogi T, Sasaki YF, Sato S, Shimada H, et al. Evaluation of the rodent micronucleus assay in the screening of IARC carcinogens (groups 1,2A and 2B) the summary report of the 6th collaborative study by CSGMT/JEMS MMS. Collaborative study of the micronucleus group test. Mammalian mutagenicity study group. Mutat Res 1997;391:259-67.
Patlolla AK, Tchounwou PB. Cytogenetic evaluation of arsenic trioxide toxicity in Sprague Dawley rats. Mutat Res 2005;587:126-33.
Pashin IV, Kozachenko VI, Toroptsev SN. Arsenic trioxide inhibition of the thiophophamide induction of mutations in mouse germ and somatic cells. Genetika 1984;20:365-66.
Deknudt G, Leonard A, Arany J, Jenar-Du-Buission G, Delavinette E. In vivo studies in male mice on the mutagenic effects of inorganic arsenic. Mutagenesis 1986;1:33-4.
Miller WH, Schipper HM, Lee JS, Singer J, Waxman S. Mechanisms of action of arsenic Trioxide. Cancer Res 2002;62:3893-903.
Bonassi S, Hagmar L, Stromberg U, Montagud AH, Tinnerberg H, Forni A, et al. Chromosomal aberrations in lymphocytes predict human cancer independently of exposure to carcinogens. Cancer Res 2000;60:1619-25.
Liou SH, Lung JC, Chen YH, Yang T, Hsieh LL, Chen CJ, et al. Increased chromosome-type chromosome aberration frequencies as biomarkers of cancer risk in a Blackfoot endemic area. Cancer Res 1999;59:1481-4.
Martinez V, Creus A, Venegas W, Arroyo A, Beck JP, Gebel TW, et al. Micronuclei assessment in buccal cells of people environmentally exposed to arsenic in northern Chile. Toxicol Lett 2005;155:319-27.
Chakraborty TD. Clastogenic effects of inorganic arsenic salts on human chromosomes in vitro. Drug Chem Toxicol 2009;32:169-73.
Salazar AM, Sordo M, Ostrosky-Wegman P. Relationship between micronuclei formation and p53 induction. Mutat Res 2009;672:124-8.
Martinez V, Creus A, Venegas W, Arroyo A, Beck JP, Gebel TW, et al. Evaluation of micronucleus induction in a Chilean population environmentally exposed to arsenic. Mutat Res 2004;564:65-74.
Tchounwou PB, Centeno JA. Toxicologic Pathology. In: Gad SC, editor. Preclinical Development Handbook. New York: John Wiley & Sons; 2008. p. 551-80.
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