ANTICANCER EFFECT OF AFRICAN LEAVES (VERNONIA AMYGDALINA DEL.) TO T47D CELL RESISTANT

Authors

  • Rialita Lifiani Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Urip Harahap Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Poppy Anjelisa Zaitun Hasibuan Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26551

Keywords:

African leaves, T47D, Resistant, Cytotoxic, 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyl tetrazolium bromide, Molecular evolution assay, Cell cycle, Apoptosis, Flow cytometry

Abstract

 Objective: African leaves (Vernonia amygdalina Del) have medical effect to threat many kind of diseases, and recently, it is suggested have anticancer effect. Therefore, this study is held to testing anticancer activity of extract African leaves to T47D cell resistant.

Method: Cytotoxic activity was determined in vitro used 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide method. Analysis of cell cycle and apoptosis were determined with flow cytometry method.

Results: Examination cytotoxicity of extract n-hexana African leaves (ENDA), extract ethyl acetate African leaves (EEADA), and extract ethanol African leaves (EEDA) to T47D cell were showed with IC50 values 164.85±1.88 μg/mL for ENDA; 55.50±0.79 μg/mL for EEADA and 311.72±4.15 μg/mL for EEDA. Therefore, EEADA has the most active activity as anticancer. Moleculer evolution assay produced T47D cell resistant. Examination toxicity of EEADA to T47D-cell resistant showed IC50 values 59.19±0.55 μg/mL. EEADA might able inhibit cell cycle at G0-G1 phase and increased apoptosis of T47D cell resistant.

Conclusion: This study shows that EEDA can be developed as cochemotheraphy to breast cancer with inhibits cel cycle and increase apoptosis.

Downloads

Download data is not yet available.

References

Shah MA, Schwartz GK. Cyclin dependent kinases as targets for cancer therapy. Update on Cancer Ther 2006;1:311-32.

Chen F, Mercado C, Yermilov I, Puig M, Ko CY, Kahn KL, et al. Improving breast cancer quality of care with the use of patient navigators. Am Surg 2010;76:1043-6.

Barnett GC, Shah M, Redman K, Easton DF, Ponder BA, Pharoah PD, et al. Risk factors for the incidence of breast cancer: Do they affect survival from the disease? J Clin Oncol 2008;26:3310-6.

Chuthapisith S, Eremin J, El-Sheemey M, Eremin O. Breast cancer chemoresistance: Emerging importance of cancer stem cells. Surg Oncol 2010;19:27-32.

Wattanapitayakul SK, Chularojmontri L, Herunsalee A, Charuchongkolwongse S, Niumsakul S, Bauer JA, et al. Screening of antioxidants from medicinal plants for cardioprotective effect against doxorubicin toxicity. Basic Clin Pharmacol Toxicol 2005;96:80-7.

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin 2015;65:5-29.

Hasibuan PA, Harahap U, Sitorus P, Satria D. Ethylacetate extract of Zanthoxylum acanthopodium DC. fruit against doxorubicin-resistanced T47D cells. Pharm Chem 2016;8:172-4.

Yeap SK, Ho WY, Beh BK, Liang WS, Ky H, Yousr AH, et al. Vernonia amygdalina, an ethnoveterinary and ethnomedical used green vegetable with multiple bioactivities. J Med Plants Res 2010;4:2787-812.

Satria D, Furqan M, Hadisahputra S, Rosidah. Combinational effects of ethylacetate extract of Picria fel-terrae lour and doxorubicin on T47d breast cancer cells. Int J Pharm Pharm Sci 2015;7:73.

Anggraeni R, Hadisahputra S, Silalahi J, Satria D. Combinational effects of ethylacetate extract of Zanthoxylum acanthopodium DC. with doxorubicin on T47D breast cancer cells. Int J Pharm Tech Res 2014;6:20-33.

Sitorus P, Satria D. Antibacterial activity of ethanol extractand volatile oil of laja gowah rhizome (Alpinia malaccensis Burm. F Roscoe.) against Staphylococcus aureus and Escherichia coli. Asian J Pharm Clin Res 2016;9:342-4.

Dalimunthe A, Achmad S, Satria D. Phenolic, flavonoid content and antioxidant activities of ethylacetate extract of Litsea cubeba (Lour.) pers. barks. Pharm Chem 2016;8:466-8.

Sihotang Y, Silalahi J, Hadisahputra S, Anjelisa P, Satria D. Cardioprotective effect of ethylacetate extract of poguntano (Picria fel-terrae Lour.) against doxorubicin-induced cardiotoxicity in rats. Int J Pharm Clin Res 2016;8:466-70.

Mosmann T. Rapid colorimetric assay forn cellular growth and survival: Aplication to proliferation and citotoxicity assays. J Immunol Methods 1983;65:55-63.

Koop F, Oak PS, Wagner E, Roid LA. miR-200c sensitizies breast cancer cells expression. PLos One 2012;7:e50469.

Hostanska K, Nisslein T, Freudenstein J, Reichling J, Saller R. Evaluation of cell death caused by triterpene glycosides and phenolic substances from Cimicifuga racemosa extract in human MCF-7 breast cancer cells. Biol Pharm Bull 2004;27:1970-5.

Meiyanto E, Handayani S, Septisetyani EP, dan Susidarti RA. Synergistic effect of Areca catechu L. ethanolic extract and its chloroform fraction with doxorubicin on MCF7. J Ilmu Kefarmasian Indonesia 2009;7:13- 8.

Kamuhabwa A, Nshimo C, de Witte P. Cytotoxicity of some medicinal plant extracts used in Tanzanian traditional medicine. J Ethnopharmacol 2000;70:143-9.

Moreno AA, Perez EA. Treatment options for breast cancer resistant to anthracycline and taxane. Mayo Clin Proc 2009;84:533-45.

Grossi F. Sequential, alternating, and maintenance/consolidation chemotherapy in advanced non-small cell lung cancer: A review of the literature. Oncologist 2007;12:451-64.

Ren W, Qiao Z, Wang H, Zhu L, dan Zhang L. Flavonoids: Promising anticancer agents. Med Res Rev 2003;23:519-34.

Petronelli A, Pannitteri G, Testa U. Triterpenoids as new promising anticancer drugs. Anticancer Drugs 2009;20:880-92.

Sung MK, dan Rao AV. Saponins as anticarcinogens. J Nutr 1995;125:717S-24.

Deferme S, dan Augustijns P. The effects of food components on the absorption of P-gp substrates: A review. J Pharm Pharmacol 2003;55:153-62.

Deferme S, Gelder JV, dan Augustijns P. Inhibitory effect of fruit extracts on pglycoprotein related efflux carriers: An in vitro screening. J Pharm Pharmacol 2002;54:1213-9.

Zhou S, Lim LY, dan Chowbay B. Herbal modulation of P-glycoprotein. Drug Metab Rev 2004;36:57-104.

Kitagawa S. Inhibitory effect of polyphenols on P-glycoprotein-mediated transport. Biol Pharm Bull 2006;29:1-6.

Gilmore TD. Introduction to NF-B: Players, pathways, perspectives. Oncogene J 2006;25:6680-4.

Ruddon RW. Cancer Biology. New York: Oxford University Press; 2007. p. 117-96.

Yadav VR, Sahdeo P, Bokyung S, Ramaswamy K, Bharat BA. Targetting inflammatory pathways by triterpenoids for prevention and treatment of cancer. Toxins 2010;2:2428-66.

Published

26-04-2018

How to Cite

Lifiani, R., U. Harahap, P. A. Z. Hasibuan, and D. Satria. “ANTICANCER EFFECT OF AFRICAN LEAVES (VERNONIA AMYGDALINA DEL.) TO T47D CELL RESISTANT”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 4-7, doi:10.22159/ajpcr.2018.v11s1.26551.

Issue

Section

Original Article(s)

Most read articles by the same author(s)

<< < 2 3 4