EFFECT OF BLACK CUMIN OIL (NIGELLA SATIVA L.) ON SPATIAL MEMORY OF ADULT MICE TREATED WITH TEMOZOLOMIDE

Zulfikri Zulfikri; Urip Harahap; Muhammad Ichwan

doi:10.22159/ajpcr.2018.v11s1.26594

Authors

Zulfikri Zulfikri Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
Urip Harahap Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
Muhammad Ichwan Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26594

Keywords:

Temozolomide, Black cumin oil, Hippocampus, Spatial memory, Morris water maze

Abstract

Â Objectives: The objective of this study is to observe the preventive effect of black cumin oil (BCO) on spatial memory impairment due to temozolomide (TMZ) treatment.

Methods: Female Swiss albino mice (n=28) were divided into four groups that received aquadest per oral (p.o), TMZ intraperitoneal (i.p) and aquadest p.o, TMZ i.p and BCO 0.1 ml p.o, and TMZ i.p and BCO 0.2 ml p.o, respectively. After 9 weeks, the mice were subjected to Morris water Maze (MWM) test. The time to find the platform (latency time) was analyzed as the indicator of memory performance.

Results: We found that mice treated with TMZ (i.p) spent significantly more time to find the platform during memory acquisition phase (on day 3) than the other groups. Interestingly, the group treated with TMZ (i.p) and BCO 0.2 ml significantly had shorter latency time than the other groups, indicating the preservation of spatial memory after TMZ treatment.

Conclusion: This result indicates that BCO can ameliorate the detrimental effect of TMZ on spatial memory in adult mice.

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