CYTOPROTECTIVE ACTIVITY OF ETHANOL FRACTION OF COLEUS AMBOINICUS LOUR. LEAVES AGAINST VERO CELLS INDUCED BY H2O2

Authors

  • Reny Haryani Departement of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Urip Harahap Departement of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Masfria Masfria Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Denny Satria Departement of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26559

Keywords:

Cytoprotective activity, Coleus amboinicus Lour, Ethanol Fraction, H2O2, Vero cells

Abstract

  Objective: The objective of this study is to assess the cytoprotective activity of Coleus amboinicus Lour. leaves ethanol fraction (EtF) in Vero cells which induced by hydrogen peroxide (H2O2).

Methods: Cytoprotective activity was determined using 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) method, apoptosis activity was analyzed by flow cytometry, and expression of radical spesies oxygen (ROS) was observed by imunocytochemistry.

Result: The EtF (100 μg/mL) was showed the highest percent viability 74.42±0.28% using MTT method. Furthermore, the EtF was increased viability of Vero cell after induced with 0.8 mM H2O2 and EtF was decreased the expression of ROS.

Conclusion: Based on the description, EtF has cytoprotective activity towards Vero cells which induced by 0.8 mM H2O2 .

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Published

26-04-2018

How to Cite

Haryani, R., U. Harahap, M. Masfria, and D. Satria. “CYTOPROTECTIVE ACTIVITY OF ETHANOL FRACTION OF COLEUS AMBOINICUS LOUR. LEAVES AGAINST VERO CELLS INDUCED BY H2O2”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 28-30, doi:10.22159/ajpcr.2018.v11s1.26559.

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