SYNERGISTIC IMMUNOMODULATORY ACTIVITY OF AQUEOUS ROOT EXTRACT OF ASPARAGUS RACEMOSUS WILLD AND ETHANOL WHOLE PLANT EXTRACT OF BOERHAVIA DIFFUSA LINN

AMRITA KUMARI; MAINAK CHAKRABORTY; NILANJAN SARKAR; SEKHAR KUMAR BOSE; KALYAN ROY; GAUTHAMAN KARUNAKARAN

doi:10.22159/ajpcr.2021.v14i11.42669

Authors

AMRITA KUMARI Department of Pharmaceutical Technology, NSHM Knowledge Campus, Kolkata, West Bengal, India.
MAINAK CHAKRABORTY Department of Pharmaceutical Technology, NSHM Knowledge Campus, Kolkata, West Bengal, India.
NILANJAN SARKAR Department of Pharmaceutical Technology, NSHM Knowledge Campus, Kolkata, West Bengal, India.
SEKHAR KUMAR BOSE Department of Pharmaceutical Technology, NSHM Knowledge Campus, Kolkata, West Bengal, India.
KALYAN ROY Department of ???, Government Pharmacy College, Sajong, Sikkim, India.
GAUTHAMAN KARUNAKARAN Department of ???, Government Pharmacy College, Sajong, Sikkim, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i11.42669

Keywords:

Immunomodulation, Carbon clearance, Neutrophil adhesion, Synergism

Abstract

Objective: On the basis of traditional use and Ethno pharmacological evidences Boerhavia diffusa whole plant and root part of Asparagus racemosus (Shatavari) both are widely used to enhance the immunity. But in combination there is no scientific evidence so current study was designed.

Methods: Laboratory based study, namely, carbon clearance, cyclophosphamide induced immune suppression and neutrophil adhesion was designed using mice as an experimental animals in different combination of aqueous whole plant extract of B. diffusa and methanol extract of root part of A. racemosus were used as a test drug in the ratio 1:1, 1:2, and 2:1 (100 mg/kg) against the established standard drug Ashwagandha.

Results: The results revealed that animals treated with combined extract (1:1, 1:2, and 2:1) at a dose of 100 mg/kg increase rate of carbon clearance from blood, there is significance alternation in blood parameter in cyclophosphamide group and also improve the Neutrophil adhesion when treated with different combination of polyherbal formulation treated groups.

Conclusion: The polyherbal formulation in different ratio showing good significant immunomodulatory activity as compare to standard.

Downloads

Download data is not yet available.

References

Sane RT. Standardisation, quality control and GMP’s for herbal drugs. Indian Drugs 2002;39:184.

Verma S, Singh SP. Current and future status of herbal medicines. Vet World 2008;1:347-50. DOI: https://doi.org/10.5455/vetworld.2008.347-350

Ravishankar B, Shukla VJ. Indian systems of medicine: A brief profile. Afr J Tradit Complement Altern Med 2007;4:319-37. DOI: https://doi.org/10.4314/ajtcam.v4i3.31226

Jennings VK, Friese B, Moore RS, Grube JW. Doubly illegal: Qualitative account so fundera geal cohol access through theft. Calif J Health Promot 2011;9:1-5. DOI: https://doi.org/10.32398/cjhp.v9i2.1431

Gayathri V, Asha V, Subramoniam A. Preliminary stuudies on the immuno-modulatory and antioxidant properties of Selaginella species. Indian J Pharmacol 2005;37:381-5. DOI: https://doi.org/10.4103/0253-7613.19075

Huang JQ, Pang MR, Li GY, Wang N, Jin L, Zhang Y. Alleviation of cyclophosphamide-induced immunosuppression in mice by naturally acetylated hemicellulose from bamboo shavings. Food Agric Immunol 2017;28:328-42. DOI: https://doi.org/10.1080/09540105.2016.1272553

Wilkinson PC. Neutrophil adhesion test. In: Vane JK, Ferreria SH, editors. Handbook of Experimental Pharmacology. 1st ed., Vol. 1. Berlin: Springer-Verlag; 1978. p. 109. DOI: https://doi.org/10.1007/978-3-642-66888-3_5

Tortora GJ, Derrickson B. Principles of Anatomy and Physiology. Vol. 11. United States, America: Wiley International; 2007. p. 820.

Patwardhan B, Kalbag D, Patki PS, Nagsampagi BA. ???. Indian Drugs 1990;28:56-3.

Pelczar MJ, Chan EC, Krieg NR. Microbiology. 5th ed. New Delhi: McGraw Hill Education; 1990. p. 703-15.

Rao CS, Raju C, Gopumadhavan S, Chauhan BL, Kulkarni RD, Mitra SK, et al. Immunotherapeutic modification by an Ayurvedic formulation Septilin. Indian J Exp Biol 1994;32:553-8.

Fulzele SV, Satturwar PM, Joshi SB, Dorle AK. Study of the immunomodulatory activity of Haridradi ghrita in rats. Indian J Pharmacol 2003;35:51-4.

van der Nat JM, Klerx JP, van Dijk H, de Silva KT, Labadie RP. Immunomodulatory activity of an aqueous extract of Azadirachta indica stem bark. J Ethnopharmacol 1987;19:125-31. DOI: https://doi.org/10.1016/0378-8741(87)90036-5

Sohni YR, Bhatt RM. Activity of a crude extract formulation in experimental hepatic amoebiasis and in immunomodulation studies. J Ethnopharmacol 1996;54:119-24. DOI: https://doi.org/10.1016/S0378-8741(96)01457-2

Kulkarni SR, Karande VS. Immunomodulatory activity of naphthaquinone extract of leaves of Lawsonia alba Linn. Indian Drugs 1998;35:427-433.

Biswas K, Chattopadhyay I, Banerjee RK, Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Curr Sci 2002;82:1336-45.

Atal CK, Sharma ML, Kaul A, Khajuria A. Immunomodulating agents of plantorigin. I: Preliminary screening. J Ethnopharmacol 1986;18:133-41. DOI: https://doi.org/10.1016/0378-8741(86)90025-5

Tiwari U, Rastogi B, Shing P, Saraf DK, Vays SP. Immunomodulatory effect of aqueous extract of Tridax procumbens in experimental animals. Ethnopharmacology 2004;92:113. DOI: https://doi.org/10.1016/j.jep.2004.02.001

Hackett CJ. Allergic rhinitis: Systemic inflammation and implications for management. Allergy Clin Immunol 2003;112:686-94. DOI: https://doi.org/10.1016/S0091-6749(03)02025-6