ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF ETHANOLIC EXTRACT OF LEAVES OF PUNICA GRANATUM L. ON EXPERIMENTAL ANIMAL MODELS

SHIPRA KAUSHIK; ABHISHEK MAHAJAN; JAYANT RAI

doi:10.22159/ajpcr.2022.v15i4.44079

Authors

SHIPRA KAUSHIK Department of Pharmacology, GS Medical College and Hospital, Pilkhuwa, Uttar Pradesh, India. https://orcid.org/0000-0002-6760-6774
ABHISHEK MAHAJAN Department of Community Medicine, GS Medical College and Hospital, Pilkhuwa, Uttar Pradesh, India.
JAYANT RAI Department of Pharmacology, GS Medical College and Hospital, Pilkhuwa, Uttar Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2022.v15i4.44079

Keywords:

Analgesic, Anti-inflammatory, Pethidine, Aspirin, Punica granatum

Abstract

Objective: The aim of the study is to evaluate the analgesic and anti-inflammatory activities of the ethanolic extract of Punica granatum L. (EEPG) on experimental animal models.

Methods: Tail-flick method was used to test the central analgesic activity, using Pethidine as standard drug. The tail flick latencies or the basal reaction time of the animals were assessed using an analgesiometer. Glacial acetic acid induced writhing response was used to test the peripheral analgesic activity, using Aspirin as standard drug. Number of writhing responses was counted for 20 min in each group and the percentage protection was calculated. And Carrageenan induced rat paw edema method was used to test anti-inflammatory activity of EEPG against acute inflammation, using Aspirin as standard drug. The inhibition of rat paw edema was calculated in percentage.

Results: In central analgesic activity, the extract and pethidine showed significant increase in the reaction time. In peripheral analgesic activity, the extract and aspirin significantly reduced the number of writhes induced by acetic acid. And in anti-inflammatory activity, the extract produced significant reduction of the carrageenan induced paw edema.

Conclusion: The EEPG has demonstrated significant analgesic and anti-inflammatory activity.

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References

Fields HL, Martin JB. Pain: Pathophysiology and management. In: Kasper DL, Braunwald E, Fauci AS, Hauser SL, Longo DL, Jameson JL, editors. Harrison’s Principles of Internal Medicine. 17th ed. New York: McGraw-Hill; 2008. p. 81-6.

Mitchell RN, Cotran RS. Acute and chronic inflammation. In: Kumar V, Cotran RS, Robbins SL, editors. Robbins Basic Pathology. 7th ed. New Delhi, India: Reed Elsevier India Pvt. Ltd.; 2003. p. 33-59.

Rao SP, Krishnamurthy V. Determination of antioxidant capacity and gallic acid content in ethanolic extract of Punica granatum L. leaf. Asian J Pharm Clin Res 2018;11:319-23. doi: 10.22159/ajpcr.2018. v11i4.24378.

Jurenka JS. Therapeutic applications of pomegranate (Punica granatum L.): A review. Altern Med Rev 2008;13:128-44. PMID 18590349

Kaur G, Jabbar Z, Athar M, Alam MS. Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe- NTA induced hepatotoxicity in mice. Food Chem Toxicol 2006;44:984- 93. doi: 10.1016/j.fct.2005.12.001, PMID 16426722

Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG,et al. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem 2005;16:360-7. doi: 10.1016/j. jnutbio.2005.01.006

Hussien NA, Abd El-Azez AM, Hamza RZ. Assessment of the genotoxic and mutagenic effect of al-Taif pomegranate (Punica granatum L) peel extract alone and combined with Malathion and atrazine pesticides in liver of male albino mice. Asian J Pharm Clin Res 2015;8:302-7.

Agarwal SS, Paridhavi M. Extraction, isolation and analysis of phytopharmaceuticals. In: Herbal Drug Technology. Himayatnagar, Hyderabad: Universities Press (India) Private Limited. 2007. p. 321-489.

OECD (Organization for Economic Cooperation and development). Section 4, Health Effects: Test No. 425: Acute Oral Toxicity: Up and Down procedure. OECD Guidelines for Testing of Chemicals. France: OECD Publishing; 2006. p. 1-27. Available from: http://www. oecdbookshop.org/oecd/index.asp?lang=en [Last accessed on 2012 Oct 27].

D’Armour FE, Smith DL. A method for determining loss of pain sensation. J Pharmacol Exp Ther 1941;72:74-9.

Witkin LB, Heubner CF, Galdi F, O’keefe E, Spitaletta P, Plummer AJ. Pharmacology of 2-amino-indane hydrochloride (Su-8629): A potent non-narcotic analgesic. J Pharmacol Exp Ther 1961;133:400-8. PMID 13831371

Ghosh MN, editor. Some common evaluation techniques. In: Fundamentals of Experimental Pharmacology. 4th ed. Kolkata, India: Scientific Book Agency; 2008. p. 162-73.

Agus S. The potency of piperine as anti inflammatory and analgesic in rats and mice. Folia Med Indones 2005;41:190-4.

Rao KV. Multiple comparison test procedures. In: Rao KV, Balakrishnan N, editors. Biostatistics. 1st ed. New Delhi, India: Jaypee Brothers Medical Publishers; 1999. p. 273-8.

Tripathi KD. Opioid analgesics and antagonists. In: Essentials of Medical Pharmacology. 6th ed. New Delhi, India: Jaypee Brothers Medical Publishers (P) Ltd.; 2008. p. 453-74.

Yaksh TL, Wallace MS. Opioids, analgesia and pain. In: Brunton L, Chabner B, Knollman B, editors. Goodman and Gillman’s the Pharmacological Basis of Therapeutics. 12th ed. New York, USA: McGraw-Hill; 2011. p. 481-515.

Satyam SM, Bairy LK, Devi V. Correlation of gender and leptin with analgesic effect of tramadol in rats. Asian J Pharm Clin Res 2018;11:493-7. doi: 10.22159/ajpcr.2018.v11i6.26678

Pise HN, Padwal SL. Diclofenac induced angioedema: A case report. Asian J Pharm Clin Res 2015;8:4-5.

Khatale PN, Manikrao AM, Vijabaskar M, Shivkumar T, Sabale PM. Analgesic and antiinflammatory activities of ethanolic extracts of Pseudarthria viscida (L) roots. Pharmacologyonline 2011;1:1153-9.