• Avinash Namdeo Jadhao Department of Biochemistry, Seth GSMC and KEMH Parel, Mumbai, Maharashtra, India.
  • Kranti Santosh Sorte Gawali Department of Biochemistry, Seth GSMC and KEMH Parel, Mumbai, Maharashtra, India.
  • Manoj Chandrakant Lokhande Department of Biochemistry, Lokmanya Tilak Municipal Medical College and General Hospital Sion, Mumbai, Maharashtra, India.
  • Parate Shravani S Department of Kayachikitsa, Ayurved Seva Sangh, Nashik, Maharashtra, India.




Cataract, PRAKRITI, Comet assay


Objectives: The Ayurvedic concept of the constitution is useful in predicting an individual’s susceptibility to age-related diseases like Cataracts (Kaphaja Linganasha). The objectives of the study were to assess DNA damage directly in human lens epithelial cells (HLEC) of senile cataracts of Vata Predominant, Pitta Predominant, and Kapha Predominant Prakriti individuals.

Methods: After obtaining Institutional Ethics Committee permission, HLEC were taken from 20 Vatta Predominant,20 Pitta Predominant and 20 Kapha Predominant Prakriti individuals of cataract after cataract surgery and from 4 controls in which quantitative assessment of DNA damage were measured using CometScore™ software. The formation of “comets” in the DNA of lens epithelial cells can be visualized through the method of single gel electrophoresis and indicates DNA strand breaks, as the damaged DNA migrates at a different rate than non-damaged DNA during electrophoresis.

Results: No such prominent comets were indicating any DNA damage in the HLEC of the four control subjects, but comets were found in cataractous HLEC. The maximal damage was found in pitta-predominant Prakriti Individuals. In senile cataract patients, in HLECs DNA was randomly damaged and this type of damage was possible by reactive oxygen species. The DNA damage in HLEC was found maximally in pitta Predominant Prakriti individuals of senile type of cataract patients. Statistical significance was observed between senile cataracts in pitta predominant Prakriti versus senile cataracts in Vata predominant Prakriti individuals and between senile cataracts in Vata predominant Prakriti versus senile cataracts in Kapha Prakriti individual. No statistically significant results were obtained for senile cataracts in pitta Prakriti versus senile cataracts in Kapha Prakriti individuals.

Conclusion: The pathogenesis of senile cataracts is multifactorial and includes continuous molecular stress brought by photo-oxidative stress, UV irradiation, and oxidative reactions.


Download data is not yet available.


Pascolini D, Mariotti SP, Pokharel GP, Pararajasegaram R, Etya’ale D, Négrel AD, et al. 2002 Global update of available data on visual impairment: A compilation of population-based prevalence studies. Ophthal Epidemiol 2004;11:67-115. doi: 10.1076/ opep., PMID 15255026

Brian G, Taylor H. Cataract blindness-challenges for the 21st century. Bull World Health Organ 2001;79:249-56. PMID 11285671

Spector A. Oxidative stress induced cataract: Mechanism of action. FASEB J 1995;9:1173-82. doi: 10.1096/fasebj.9.12.7672510, PMID 7672510

Wang X, Simpkins JW, Dykens JA, Cammarata PR. Oxidative damage to human lens epithelial cells in culture: Estrogen protection of mitochondrial potential, ATP and cell viability. Invest Ophthalmol Vis Sci 2003;44:2067-75. doi: 10.1167/iovs.02-0841, PMID 12714645

Ortwerth BJ, Coots A, James HL, Linetsky M. UVA irradiation of human lens proteins produces residual oxidation of ascorbic acid even in the presence of high levels of glutathione. Arch Biochem Biophys 1998;351:189-96. doi: 10.1006/abbi.1997.0549, PMID 9515056

Jose JG, Yielding KL. “Unscheduled” DNA synthesis in lens epithelium following ultraviolet radiation. Exp Eye Res 1977;24:113-9. doi: 10.1016/0014-4835(77)90252-4, PMID 851489

Kojima M. Regional enzymatic analysis of UV-B and streptozotocin induced diabetic cataract lens. Lens Eye Toxic Res 1990;7:547-61. PMID 1966039

Kuck JF Jr. Effect of long wave ultraviolet light on the lens. IV. Leucine metabolism in normal human lenses in vitro Ophthalmic Res 1983;15:216-9. doi: 10.1159/000265262, PMID 6634057

Dhiman KS, Dhiman K, Puri S, Ahuja D. A comprehensive review of cataract (Kaphaja Linganasha) and its surgical treatment in ayurvedic literature. Ayu 2010;31:93-100. doi: 10.4103/0974-8520.68197, PMID 22131692, PMCID PMC3215330

Tripathi PK, Gehlot S. Development, validation and confirmation of an archetype tool to evaluate Prakriti. J Nat Rem 2019;19:206-13. doi:10.18311/jnr/2019/23788

Gajjar D, Patel D, Alapure B, Praveen MR, Patel A, Johar K, et al. Rapid action of oestradiol against hydrogen peroxide induced oxidative stress in cataractous lens epithelium: An in vitro study. Eye (Lond) 2008;23:1456-63.

Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterström C. Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract. J Cataract Refract Surg 2006;32:1483-8. doi: 10.1016/j.jcrs.2006.04.017, PMID 16931259

Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-91. doi: 10.1016/0014-4827(88)90265-0, PMID 3345800

Bhat SP. The ocular lens epithelium. Biosci Rep 2001;21:537-63. doi: 10.1023/a:1017952128502, PMID 11900326

Bassnett S, McNulty R. The effect of elevated intraocular oxygen on organelle degradation in the embryonic chicken lens. J Exp Biol 2003;206:4353-61. doi: 10.1242/jeb.00670, PMID 14581604

Eaton JW. Is the lens canned? Free Radic Biol Med 1991;11:207-13. doi: 10.1016/0891-5849(91)90173-z, PMID 1937139

Holekamp NM, Shui YB, Beebe DC. Vitrectomy surgery increases oxygen exposure to the lens: A possible mechanism for nuclear cataract formation. Am J Ophthalmol 2005;139:302-10. doi: 10.1016/j. ajo.2004.09.046, PMID 15733992

Shui YB, Fu JJ, Garcia C, Dattilo LK, Rajagopal R, McMillan S, et al. Oxygen distribution in the rabbit eye and oxygen consumption by the lens. Invest Ophthalmol Vis Sci 2006;47:1571-80. doi: 10.1167/ iovs.05-1475, PMID 16565394

Vinson JA. Oxidative stress in cataracts. Pathophysiology 2006;13:151-62. doi: 10.1016/j.pathophys.2006.05.006, PMID 16765571

Tang Y, Wang X, Wang J, Jin L, Huang W, Luo Y, et al. Risk factors of age-related cataract in a Chinese adult population: The Taizhou eye study. Clin Exp Ophthalmol 2018;46:371-9. doi: 10.1111/ceo.13040, PMID 28842942

Purvya MC, Meena MS. A review on role of Prakriti in aging. Ayu 2011;32:20-4. doi: 10.4103/0974-8520.85719, PMID 22131753



How to Cite

Jadhao, A. N., K. Santosh Sorte Gawali, M. Chandrakant Lokhande, and P. Shravani S. “DNA DAMAGE IN LENS EPITHELIAL CELLS OF SENILE CATARACT PATIENTS OF DIFFERENT PRAKRITI ACCORDING TO AYURVEDA LITERATURE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 16, no. 3, Mar. 2023, pp. 77-80, doi:10.22159/ajpcr.2023.v16i3.46712.



Original Article(s)