DEVELOPMENT AND IN VITRO EVALUATION OF PHYTOSOMES OF ELLAGIC ACID
DOI:
https://doi.org/10.22159/ajpcr.2023.v16i3.47129Keywords:
Phytosomes, Bioavailability, Anti-solvent precipitation, DissolutionAbstract
Objectives: The main objective of the present work is to enhance the bioavailability of ellagic acid (EA) by increasing its dissolution there by allowing for the exploitation of its therapeutic effects.
Methods: Phytosomes containing EA were prepared by anti-solvent precipitation method. The prepared phytosomes were evaluated for drug entrapment efficiency, in vitro drug release, and drug excipient interaction studies.
Results: Formulation F2 containing EA and soya lecithin in the ratio (1:2) showed highest percentage of drug release as 85.40% in 60 min and 95.86% in 120 min. The drug entrapment efficiency values were satisfactory. There were no interactions between the drug and the excipients used in its preparation according to Fourier-transform infrared spectra of pure EA and EA phytosomes.
Conclusion: Phytosomes of EA were successfully produced by anti-solvent precipitation method and the percentage drug entrapment efficiency was satisfactory in almost all formulations. Formulation F2 exhibited highest percent of drug release as 85.40% in 60 min and 95.86% in 120 min to possess optimum bioavailability.
Downloads
References
National Centre for Biotechnology Information. PubChem Compound Summary for CID 5281855, Ellagic Acid. PubChem [Internet]. Bethesda (MD): National Library of Medicine (US).
Ceci C, Graziani G, Faraoni I, Cacciotti I. Strategies to improve ellagic acid bioavailability: From natural or semisynthetic derivatives to nanotechnological approaches based on innovative carriers. Nanotechnology 2020;31:382001.
Ceci C, Lacal P, Tentori L, De Martino M, Miano R, Graziani G. Experimental evidence of the antitumor, antimetastatic and antiangiogenic activity of ellagic acid. Nutrients 2018;10:1756.
Larrosa M, García-Conesa MT, Espín JC, Tomás-Barberán FA. Ellagitannins, ellagic acid and vascular health. Mol Aspects Med 2010;31:513-39.
Agrawal OD, Kulkarni YA. Mini-review of analytical methods used in quantification of ellagic acid. Rev Anal Chem 2020;39:31-44.
Ratnam DV, Ankola DD, Bhardwaj V, Sahana DK, Kumar MN. Role of antioxidants in prophylaxis and therapy: A pharmaceutical perspective. J Control Release 2006;113:189-207.
Mo J, Panichayupakaranant P, Kaewnopparat N, Nitiruangjaras A, Reanmongkol W. Wound healing activities of standardized pomegranate rind extract and its major antioxidant ellagic acid in rat dermal wounds. J Nat Med 2014;68:377-86.
Castonguay A, Boukharta M, Teel R. Biodistribution of antimutagenic efficacies in salmonella typhimurium of, and inhibition of P450 activities by ellagic acid and one analogue. Chem Res Toxicol 1998;11:1258-64.
Madrigal-Carballo S, Lim S, Rodriguez G, Vila AO, Krueger CG, Gunasekaran S, et al. Biopolymer coating of soybean lecithin liposomes via layer-by-layer self-assembly as novel delivery system for ellagic acid. J Funct Foods 2010;2:99-106.
Bala I, Bhardwaj V, Hariharan S, Sitterberg J, Bakowsky U, Kumar MN. Design of biodegradable nanoparticles: A novel approach to encapsulating poorly soluble phytochemical ellagic acid. Nanotechnology 2005;16:2819-22.
Arulmozhi V, Pandian K, Mirunalini S. Ellagic acid encapsulated chitosan nanoparticles for drug delivery system in human oral cancer cell line (KB). Colloids Surf B Biointerfaces 2013;110:313-20.
Suri S, Mirza MA, Anwer MK, Alshetaili AS, Alshahrani SM, Ahmed FJ, et al. Development of NIPAAm-PEG acrylate polymeric nanoparticles for co-delivery of paclitaxel with ellagic acid for the treatment of breast cancer. J Pol Eng 2019;39:271-8.
Barani M, Sangiovanni E, Angarano M, Rajizadeh MA, Mehrabani M, Piazza S, et al. Phytosomes as innovative delivery systems for phytochemicals: A comprehensive review of literature. Int J Nanomedicine 2021;16:6983-7022.
Lu M, Qiu Q, Luo X, Liu X, Sun J, Wang C, et al. Phyto-phospholipid complexes (phytosomes): A novel strategy to improve the bioavailability of active constituents. Asian J Pharm Sci 2019;14:265-74.
Jyothi BJ, Ragalatha PM. Development and in vitro evaluation of phytosomes of naringin. Asian J Pharm Clin Res 2019;12:252-6.
Bruschi ML. Mathematical models of drug release. In: Strategies to Modify the Drug Release from Pharmaceutical Systems. Sawston, United Kingdom: Woodhead Publishing; 2015. p. 63-86.
Published
How to Cite
Issue
Section
Copyright (c) 2022 Ramya K, Jeevana Jyothi.B
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
