EVALUATION OF THE ANTITYROSINASE AND ANTIOXIDANT POTENTIAL OF ZINC OXIDE NANOPARTICLES SYNTHESIZED FROM THE BROWN SEAWEED-TURBINARIA CONOIDES
DOI:
https://doi.org/10.22159/ijap.2017v9i5.20847Keywords:
Antityrosinase activity, SEM-EDAX, FRAP, Turbinaria conoidesAbstract
Objective: The present study was designed to evaluate the antioxidant and antityrosinase properties of zinc oxide nanoparticles (ZnO-NPs) synthesized from brown seaweed Turbinaria conoides.
Methods: Zinc Oxide Nanoparticles were synthesized from the hydroethanolic extract of Turbinaria conoides. Ultraviolet-Visible Spectrophotometric analysis was performed to confirm the formation of ZnO-NPs. Size, morphology and elemental composition of ZnO-NPs were analysed using SEM-EDAX. The antioxidant activity of the synthesized zinc oxide nanoparticles was investigated by total antioxidant capacity (phosphomolybdenum method), reducing power assay and ferric reducing antioxidant power assay (FRAP). Anti tyrosinase activity was assessed to validate the skin whitening ability of the ZnO-NPs.
Results: The antioxidant activity of ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides was maximum when compared with that of the hydroethanolic algal extract. The antityrosinase activity of ZnO-NPs was found to be maximum with 75% tyrosinase inhibition when compared to hydroethanolic algal extract which had 56% inhibition at 250μg/ml concentration.
Conclusion: Overall our study provides a firm evidence to support that antityrosinase and antioxidant activities are exhibited by ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides and it might be used as an antioxidant and as a source of skin whitening agent in cosmetics.
Downloads
References
Braverman IM, Fonferko E. Studies in cutaneous aging: the elastic fiber network. J Invest Dermatol 1982;78:434-43.
Hearing VJ. Biogenesis of pigment granulesa sensitive way to regulate melanocyte function. J Dermatol Sci 2005;37:3-14.
Briganti S, Camera E, Picardo M. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res 2003;16:101-10.
Sturm RA, Tesdeal RD, Box NF. Human pigmentation genes identification, structureand consequences of polymorphic variation. Gene 2001;277:49-62.
Hu ZM, Zhou Q, Lei TC, Ding SF, Xu SZ. Effects of hydroquinone and its glucoside derivatives on melanogenesis and antioxidation: biosafety as skin whitening agents. J Dermatol Sci 2009;55:179–84.
Kausar R, Akhtar N. Evaluation of depegmenting and antierythemic effects of cosmetic emulgels containing raspberry fruit extract on human cheek skin. Int J Pharm Pharm Sci 2017;9:236-8.
Raper HS. The anaerobic oxidase. Physiol Rev 1928;8:245-82.
Parvez S, Kang M, Chung HS, Cho C, Hong MC, Shin K, Bae H. Survey and mechanism of skin depigmenting and lightening agents. Phytother Res 2006;20:921-34.
Quishi C. Evaluate the effectiveness of the natural cosmetic product compared to chemical-based product. Int J Chem 2009;1:57-9.
Nerya O, Vaya J, Musa R, Izrael S, Ben-Arie R, Tamir S. Glabrene and isoliquiritigenin as tyrosinase inhibitors from licorice roots. J Agric Food Chem 2003;51:1201–7.
Wiesenthal A, Hunter L, Wang S, Wickliffe J, Wilkerson M. Nanoparticles: small and mighty. Int J Dermatol 2011;50:247–54.
Lorenz C, Tiede K, Tear S, Boxall A, Von Goetz N, Hungerbühler K. Imaging and characterization of engineered nanoparticles in sunscreens by electron microscopy, under wet and dry conditions. Int J Occup Environ Health 2010;16:406–8.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of phosphomolybdenum complex: specific application to determination of vitamin E. Anal Biochem 1999;269:337-41.
Oyaizu M. Studies on products of browning reactions: Antioxidative activities of products of browning reactions prepared from glucoamine. Jpn J Nutr 1986;4:307-15.
Okonogi S, Duangrat C, Anuchpreeda S, Tachakittirungrod S, Chowwanapoonpohn S. Comparison of antioxidant capacities and cytotoxicities of certain fruit peels. Food Chem 2007;103:839-46.
Friedman M. Chemistry, biochemistry and dietary role of potato polyphenols. J Agric Food Chem 1997;45:1523-40.
Vanni A, Gastaldi D. Kinetic investigation on the double enzymic activity of the tyrosinase mushroom. Ann Chim 1990;80:35-60.
Kiken DA, Cohen DE. Contact dermatitis to botanical extracts. Am J Contact Dermat 2002;13:148-52.
Saewan N, Thakam A, Jintaisong A, Kittigowitana K. Anti-tyrosinase and cytotoxicity activities of curcumin-metal complexes. Int J Pharm Pharm Sci 2014;6:270-3.
Lu PJ, Huang SC, Chen YP, Chiueh LC, Shih DYC. Analysis of titanium dioxide and zinc oxide nanoparticles in cosmetics. J Food Drug Anal 2015;23:587-94.
Pintus F, Span OD, Corona A, Medda R. Antityrosinase activity of Euphorbia characias extracts. Peer J 2015. Doi:10.7717/peerj.1305.
Basavegowda N, Mishra K, Lee YR, Kim SH. Antioxidant and anti-tyrosinase activities of palladium nanoparticles synthesized using saururus chinensis. J Clust Sci 2015;27:1-13.
Salleh W, Ahmad F, Yen KH. Antioxidant and anti-tyrosinase activities from Piper officinarum (Piperaceae). J Appl Pharm Sci 2014;4:87-91.
Govindappa M, Farheen M, Chandrappa CP, Channabasava, Ravishankar V Rai, Raghavendra VB. Mycosynthesis of silver nanoparticles using extract of endophytic fungi, penicillium species of Glycosmis mauritiana, and its antioxidant, antimicrobial, anti-inflammatory and tyrokinase inhibitory activity. Adv Natl Sci: Nanosci Nanotechnol 2016;7:1-9.
Sherikar AS, Mahanthesh MC. Evaluation of aqueous and methanolic extract of leaves of epipremnum aureum for radical scavenging activity by DPPH Method, total phenolic content, reducing capacity assay and FRAP assay. J Pharmacogn Phytochem 2015;4:36-40.
Dennog C, Radermacher P, Barnett YA, Speit G. Antioxidant status in humans after exposure to hyperbaric oxygen. Mutat Res 1999;428:83–9.
Niemeyer HB, Metzler M. Differences in the antioxidant activity of plant and mammalian lignans. J Food Eng 2003;56:255-6.
Published
How to Cite
Issue
Section
