ANALYSIS OF THE INHIBITORY POTENTIAL OF STREPTOCOCCUS SALIVARIUS ISOLATED FROM ADULT SALIVA AND THE TONGUE DORSUM FOR THE GROWTH OF CANDIDA ALBICANS
DOI:
https://doi.org/10.22159/ijap.2017.v9s1.05Keywords:
Bacteriocins, Candida albicans, Probiotic, Protein, Streptococcus salivariusAbstract
Objectives: The objective of this study is to analyze the effectiveness of Streptococcus salivarius and its protein for inhibiting the growth of Candida
albicans.
Methods: The analysis was conducted using polymerase chain reaction, sodium dodecyl sulfate polyacrylamide gel electrophoresis, a Bradford test,
deferred antagonism test, and well-diffusion agar.
Result: S. salivarius, isolated from saliva and the tongue dorsum, and its protein do not inhibit the growth of C. albicans. The morphology of C. albicans
did not change after being exposed to protein produced by S. salivarius.
Conclusions: S. salivarius and its protein do not inhibit the growth of C. albicans. However, the bacterium has the capacity to maintain fungus
morphology in the form of blastospora.
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References
Levy SB. The 2000 Garrod lecture. Factors impacting on the problem of antibiotic resistance. J Antimicrob Chemother 2002;49(1):25-30.
Horz HP, Meinelt A, Houben B, Conrads G. Distribution and persistence of probiotic Streptococcus salivarius K12 in the human oral cavity as determined by real-time quantitative polymerase chain reaction. Oral Microbiol Immunol 2007;22(2):126-30.
Fauzi AA, Shafiei Z, Baharin B, Mohd N. Isolation of Lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens. Sains Malays 2013;1:19-24.
Wescombe PA, Hale JD, Heng NC, Tagg JR. Developing oral probiotics from Streptococcus salivarius. Future Microbiol 2012;7(12):1355-71.
Ishijima SA, Hayama K, Burton JP, Reid G, Okada M, Matsushita Y, et al. Effect of Streptococcus salivarius K12 on the in vitro growth of Candida albicans and its protective effect in an oral candidiasis model. Appl Environ Microbiol 2012;78(7):2190-9.
Samaranayake L. Essential Microbiology for Dentistry. 4th ed. Toronto: Churchill Livingstone Elsevier Ltd.; 2012.
Yamaguchi M, Tezuka Y, Takeda K, Shetty V. Disposable collection kit for rapid and reliable collection of saliva. Am J Hum Biol 2015;27(5):720-3.
Wescombe PA, Upton M, Dierksen KP, Ragland NL, Sivabalan S, Wirawan RE, et al. Production of the lantibiotic salivaricin a and its variants by oral streptococci and use of a specific induction assay to detect their presence in human saliva. Appl Environ Microbiol 2006;72(2):1459-66.
Köhler GA, Assefa S, Reid G. Probiotic interference of Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 with the opportunistic fungal pathogen Candida albicans. Infect Dis Obstet Gynecol 2012;2012:14.
Salimetrics LL. Saliva collection and handling advice. Methods 2009;44:14.
Barbour A, Philip K. Variable characteristics of bacteriocin-producing Streptococcus salivarius strains isolated from Malaysian subjects. PLoS One 2014;9(6):e100541.
Santagati M, Scillato M, Patanè F, Aiello C, Stefani S. Bacteriocinproducing oral streptococci and inhibition of respiratory pathogens. FEMS Immunol Med Microbiol 2012;65(1):23-31.
Bowe WP, Filip JC, DiRienzo JM, Volgina A, Margolis DJ. Inhibition of propionibacterium acnes by bacteriocin-like inhibitory substances (BLIS) produced by Streptococcus salivarius. J Drugs Dermatol 2006;5(9):868-70.
Johansson A. Aggregatibacter actinomycetemcomitans leukotoxin: A powerful tool with capacity to cause imbalance in the host inflammatory response. Toxins (Basel) 2011;3(3):242-59.
Prasad S, Morris PC, Hansen R, Meaden PG, Austin B. A novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio harveyi. Microbiology 2005;151:3051-8.
Yang E, Fan L, Jiang Y, Doucette C, Fillmore S. Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. AMB Express 2012;2(1):48.
Roger P, Delettre J, Bouix M, Béal C. Characterization of Streptococcus salivarius growth and maintenance in artificial saliva. J Appl Microbiol 2011;111(3):631-41.
Morales DK, Hogan DA. Candida albicans interactions with bacteria in the context of human health and disease. PLoS Pathog 2010;6(4):e1000886.
Shirtliff ME, Peters BM, Jabra-Rizk MA. Cross-kingdom interactions:
Candida albicans and bacteria. FEMS Microbiol Lett 2009;299(1):1-8.
Diaz PI, Xie Z, Sobue T, Thompson A, Biyikoglu B, Ricker A, et al. Synergistic interaction between Candida albicans and commensal oral streptococci in a novel in vitro mucosal model. Infect Immun 2012;80(2):620-32.
MacDonald KW. The Role of Streptococcus salivarius as a Modulator of Homeostasis in the Oral Cavity. Electronic Thesis and Dissertation Repository. University of Western Ontario; 2015.
Ogawa A, Furukawa S, Fujita S, Mitobe J, Kawarai T, Narisawa N, et al. Inhibition of streptococcus mutans biofilm formation by streptococcus salivarius FruA. Appl Environ Microbiol. 2011;77(5):1572-80.