• Nuraini Azizah Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
  • Erny Sagita Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
  • Iskandarsyah Iskandarsyah Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.



Capsaicin, Franz diffusion cell, Gel, Penetration, Transethosome


Objective: Capsaicin is an active compound found in chili pepper and has been shown to have analgesic, antioxidant, anticancer, and anti-obesity
properties. To improve its penetration into the skin, capsaicin was prepared in a transethosome vesicle. Importantly, transethosomes are vesicles that
consist of phosphatidylcholine, surfactant, and ethanol. In this study, capsaicin was prepared in a transethosome vesicle using two different methods:
Direct transethosome formation and thin layer hydration. The aims of this study were to determine the effects of various methods of transethosome
formation on capsaicin characteristics and to evaluate the penetration capabilities of transethosome capsaicin gel.
Methods: Ultimately, transethosome formation via the thin layer method yielded more favorable characteristics; these formations had particle sizes
of 174.9±2.02 nm and an entrapment efficiency of 84.85±1.15%. The transethosome suspension was then developed into a gel formulation using 1%
carbomer. An in vitro penetration test was performed using a Franz diffusion cell of mice abdomen skin, and the performance of the transethosome
capsaicin gel was compared to that of the standard capsaicin gel.
Results: Penetration rate of capsaicin from either the transethosome gel preparation and the standard gel substance was 1549.68±49.6 and
846.05±10.1 μg/cm2, respectively.
Conclusions: According to these results, it can be concluded that gel preparations containing transethosome increase capsaicin penetration into the


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Kimura H, Ogawa S, Sugiyama A, Jisaka M, Takeuchi T, Yokota K.

Anti-obesity effects of highly polymeric proanthocyanidins from seed

shells of Japanese horse chestnut (Aesculus turbinata Blume). Food Res

Int 2011;44(1):121-6.

Whiting S, Derbyshire E, Tiwari BK. Capsaicinoids and capsinoids.

A potential role for weight management? A systematic review of the

evidence. Appetite 2012;59(2):341-8.

Sarwa KK, Mazumder B, Rudrapal M, Verma VK. Potential

of capsaicin-loaded transfersomes in arthritic rats. Drug Deliv


Ascenso A, Raposo S, Batista C, Cardoso P, Mendes T, Praça FG,

et al. Development, characterization, and skin delivery studies of

related ultradeformable vesicles: Transfersomes, ethosomes, and

transethosomes. Int J Nanomedicine 2015;10:5837-1.

Song CK, Balakrishnan P, Shim CK, Chung SJ, Chong S, Kim DD.

A novel vesicular carrier, transethosome, for enhanced skin delivery of

voriconazole: Characterization and in vitro/in vivo evaluation. Colloids

Surf B Biointerfaces 2012;92:299-304.

Ma M, Wang J, Guo F, Lei M, Tan F, Li N. Development of

nanovesicular systems for dermal imiquimod delivery: Physicochemical

characterization and in vitro/in vivo evaluation. J Mater Sci Mater Med


Shaji J, Garude S. Transethosomes and ethosomes for enhanced

transdermal delivery of ketorolac tromethamine. Comp Assess


A Guidebook to Particle Size Analysis. Available from: https://www.

Guidebook.pdf; 2014.

Chen Y, Wu Q, Zhang Z, Yuan L, Liu X, Zhou L. Preparation of

curcumin-loaded liposomes and evaluation of their skin permeation and

pharmacodynamics. Molecules 2012;17(5):5972-87.

El Zaafarany GM, Awad GA, Holayel SM, Mortada ND. Role of edge

activators and surface charge in developing ultradeformable vesicles

with enhanced skin delivery. Int J Pharm 2010;397(1-2):164-72.

Tranggoono RI, Latifah F. Handbook of Cosmetics Study. Jakarta:

Gramedia; 2007.

Barry BW. Novel mechanisms and devices to enable successful

transdermal drug delivery. Eur J Pharm Sci 2001;14(2):101-4.



How to Cite

Azizah, N., Sagita, E., & Iskandarsyah, I. (2017). IN VITRO PENETRATION TESTS OF TRANSETHOSOME GEL PREPARATIONS CONTAINING CAPSAICIN. International Journal of Applied Pharmaceutics, 9, 116–119.



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