METHOD DEVELOPMENT AND VALIDATION FOR ANALYSIS OF DEOXYARBUTININ ANHYDROUS EMULSION SYSTEM USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

MUCHTARIDI MUCHTARIDI; IDA MUSFIROH; AHMAD FAUZI

doi:10.22159/ijap.2019v11i2.30461

Authors

MUCHTARIDI MUCHTARIDI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjadjaran, Sumedang, Indonesia http://orcid.org/0000-0002-6156-8025
IDA MUSFIROH Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjadjaran, Sumedang, Indonesia
AHMAD FAUZI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjadjaran, Sumedang, Indonesia

DOI:

https://doi.org/10.22159/ijap.2019v11i2.30461

Keywords:

Analytical method validation, Anhydrous emulsion, Deoxyarbutin

Abstract

Objective: The aim of this study is to develop a simple, precise and accurate analytical method of deoxyarbutin in anhydrous emulsion system preparation.

Methods: The analysis was conducted using high-performance liquid chromatography (HPLC). Chromatographic analysis was carried out using a reversed phase-C18 column. The mobile consists of two phases methanol and water (60: 40 v/v) at a flow rate of 1.0 ml/min. The determinations were performed using UV detector set at 225 nm. All validation procedures were added with hydroquinone as an internal standard.

Results: The method showed coefficient correlation is 0.9978, relative standard deviation (RSD) smaller than 2%, Limit of Detection (LOD) and Limit of Quantitation (LOQ) are 0.599 µg/ml and 1.817 µg/ml respectively. The total amount deoxyarbutin in anhydrous emulsion preparation is 1.964+0.02 % with 98% recovery percentage.

Conclusion: The developed HPLC analytical method meets the validation criteria made by International Conference on Harmonisation (ICH).