VALIDATION OF ANALYTICAL METHOD OF 2,5-HEXANEDIONE ON URINE BY GAS CHROMATOGRAPHY

MUCHTARIDI MUCHTARIDI; Kurnia Megawati; Febrina Amelia Saputri; Mulyana M.

doi:10.22159/ijap.2019v11i4.32687

Authors

MUCHTARIDI MUCHTARIDI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang Km 21 Jatinangor, West Java, Indonesia, 45363 http://orcid.org/0000-0002-6156-8025
Kurnia Megawati Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang Km 21 Jatinangor, West Java, Indonesia, 45363
Febrina Amelia Saputri Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang Km 21 Jatinangor, West Java, Indonesia, 45363
Mulyana M. PT. Prodia OHI International, Jalan Kramat Raya No. 148, Central Jakarta 10430, Indonesia

DOI:

https://doi.org/10.22159/ijap.2019v11i4.32687

Keywords:

2,5-hexanedione, Gas chromatography, Hexane, Validation

Abstract

Objective: The purpose of this study was to obtain a valid analytical method for determining the level of 2,5-hexanedione in the urine of oil industry workers.

Methods: Gas Chromatography (GC) was employed to analyze 2,5-hexanedione in the urine. The analysis was done using HP-5 (Crosslinked methyl siloxane) capillary columns 30 m x 0.320 mm long, film thickness 0.25 μm. The temperature of the detector temperature was 300 °C, and the injector temperature was 250 °C. The helium gas flow rate was 2 ml/min. The detector was Flame Ionization Detection (FID). Parameters of system suitability test and validation were obtained.

Results: This study results that the method of analysis 2,5-hexanedione in urine by Gas Chromatography (GC) confirm the requirements of the validation method with a linearity was 0.99963, accuracy was in the range of 99.16% to 114.13%, the precision with % coefficient of variation was 1.65% to 5.16%, % coefficient variation of specificity was 0.027%, limit of detection was 0.054 μg/ml and limit of quantification was 0.18 μg/ml.

Conclusion: The proposed GC method meets the acceptance criteria of validation parameters and can be applied for routine analysis.

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