THE OPTIMIZATION OF HPLC FOR QUANTITATIVE ANALYSIS OF ACID ORANGE 7 AND SUDAN II IN COSMETIC PRODUCTS USING BOX BEHNKEN DESIGN

Authors

  • NOVALINA B. R. PURBA Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
  • ABDUL ROHMAN Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
  • SUDIBYO MARTONO Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta

DOI:

https://doi.org/10.22159/ijap.2019v11i2.31285

Keywords:

Acid orange 7, Sudan II, HPLC, BBD, Blusher

Abstract

Objective: The objective of this study was to optimize high-performance liquid chromatography (HPLC) method for the determination of acid orange 7 (AO7) and sudan II (SII) in blusher product based on response surface methodology using box behnken design (BBD) approach.

Methods: Some factors responsible for HPLC separation including column temperature, mobile phase composition, flow rate were optimized using BBD. The responses evaluated were peak area, retention time, and tailing factor. AO7 and SII in blusher product has different properties, therefore both analytes were analysed using C18 column (Thermo Synergy Gold 250 mm x 4.6 mm i.d.,5 µm) using Shimadzu LC 20AD chromatograph equipped with photo-diode array (PDA) detector at 300-650 nm. The mobile phase used was acetonitrile-water (1:1 v/v), and acetonitrile composition was optimized at 35-50% for separation AO7 (ACN1), and 80-90% for SII (ACN2), delivered at the flow rate of 0.9–1 ml/min, using column temperature at 30-40 °C.

Results: BBD showed that separation of AO7 was influenced by the concentration of ACN1, flow rate and column temperature. These factors affected retention time, peak area, and tailing factor with peak area was the most significant. Tailing factor was not significantly affected by each factor, and retention time was slightly effected. Otherwise, Sudan II was affected by all these factors except ACN1. The optimal condition obtained based BBD was ACN1 43%, ACN2 90%, the flow rate of 0.9 ml/min and a column temperature of 40 °C.

Conclusion: BBD can be used to get optimum condition for analysis of AO7 and SII in blusher product.

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References

Kanekar H, Khale A. Coloring agents: a current regulatory perspective for coloring agents intended for pharmaceutical and cosmetic use. Int J Pharm Phytopharmacol Res 2014;2:1–20.

Rebane R, Leito I, Yurchenko S, Herodes K. A review of analytical techniques for determination of sudan I–IV dyes in food matrixes. J Chromatogr A 2010;1217:2747–57.

Barot J, Bahadur A. Toxic impacts of C. I. acid orange 7 on behavioural, haematological and some biochemical parameters of labeo rohita fingerlings. Int J Sci Res Environ Sci 2015;3:284–90.

Pan H, Feng J, Cerniglia CE, Chen H. Effects of orange II and sudan III azo dyes and their metabolites on Staphylococcus Aureusi. J Industr Microb Biotechnol 2011;38:1729–38.

Anfossi L, Baggiani C, Giovannoli C, Giraudi G. Development of enzyme-linked immunosorbent assays for sudan dyes in chilli powder, ketchup and egg yolk. Food Addit Contam 2009;26:800–7.

Di Anibal CV, Odena M, Ruisánchez I, Callao MP. Determining the adulteration of spices with Sudan I-II-II-IV dyes by UV–visible spectroscopy and multivariate classification techniques. Talanta 2009;79:887–92.

Lohumi S, Josh R, Kandpal LM, Lee H, Kim MS, Cho H. Quantitative analysis of sudan dye adulteration in paprika powder using FTIR spectroscopy. Food Addit Contam Part A 2017;34:678-86.

Schwack W, Pellissier E, Morlock G. Analysis of unauthorized sudan dyes in food by high-performance thin layer chromatography. Anal Bioanal Chem 2018;410:5641–51.

He L, Su Y, Fang B, Shen X, Zeng Z, Liu Y. Determination of sudan dye residues in eggs by liquid chromatography and gas chromatography-mass spectrometry. Anal Chim Acta 2007;594:139–46.

Zhao S, Yin J, Zhang J, Ding X, Wu Y, Shao B. Determination of 23 dyes in chili powder and paste by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Food Anal Methods 2012;5:1018–26.

Cornet V, Govaert Y, Moens G, Van Loco J, Degroodt JM. Development of a fast analytical method for the determination of sudan dyes in chili and curry containing foodstuffs by high performance liquid chromatography-photodiode array detection. J Agric Food Chem 2006;54:639–44.

Ertas E, Ozer H, Alasalvar C. A rapid HPLC method for determination of sudan dyes and para red in red chilli pepper. Food Chem 2007;105:756–60.

Van Bommel MR, Berghe IV, Wallert AM, Boitelle R, Wouters J. High-performance liquid chromatography and non-destructive three-dimensional fluorescence analysis of early synthetic dyes. J Chromatogr A 2007;1157:260–72.

Abbott LC, Batchelor SN, Smith JRL, Moore JN. Reductive reaction mechanisms of the azo dye orange II in aqueous solution and in cellulose: from radical intermediates to products. J Phys Chem A 2009;113:6091–103.

Rejczak T, Tuzimski T. Application of high-performance liquid chromatography with diode array detector for simultaneous determination of 11 synthetic dyes in selected beverages and foodstuffs. Food Anal Methods 2017;10:3572–88.

Shaji J, Shah A. Optimization of tenoxicam loaded niosomes using quadratic design. Int J Curr Pharm Res 2016;8:67–62.

Khanam N, Alam MI, Iqbal QMA, Ali MY, Siddiqui AR. A review on optimization of drug delivery system with experimental designs. Int J Appl Pharm 2018;10:12-7.

Siregar C, Prabaningdyah NK, Choiri S, Riyanto S, Rohman A. Optimization of HPLC using central composite design for determination of curcumin and demethoxycurcumin in tablet dosage form. Dhaka Univ J Pharm Sci 2018;16:137-45.

Setyawan EH, Setyowati EP, Rohman A, Nugroho AK. Central composite design for optimizing extraction of EGCG from green tea leaf (Camellia sinensis L.). Int J Appl Pharm 2018;10:211–6.

Bezerra MA, Santelli RE, Oliveira EP, Villar LS, Escaleira LA. Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta 2008;76:965–77.

Qiu P. Application of box-behnken design with response surface methodology for modeling and optimizing ultrasonic oxidation of arsenite with H2O2. Carr Eur J Chem 2014;12:172–64.

Sadhukhan B, Mondal NK, Chattoraj S. Optimisation using central composite design (CCD) and the desirability function for sorption of methylene blue from aqueous solution onto lemna major. Karbala Int J Modern Sci 2016;2:155–45.

Published

07-03-2019

How to Cite

PURBA, N. B. R., ROHMAN, A., & MARTONO, S. (2019). THE OPTIMIZATION OF HPLC FOR QUANTITATIVE ANALYSIS OF ACID ORANGE 7 AND SUDAN II IN COSMETIC PRODUCTS USING BOX BEHNKEN DESIGN. International Journal of Applied Pharmaceutics, 11(2), 130–137. https://doi.org/10.22159/ijap.2019v11i2.31285

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