ANTIOXIDANT ACTIVITY OF THE ASCIDIAN MARINE INVERTEBRATES, DIDEMNUM SP.

Authors

  • Berna Elya Center for Natural Product Medicine Studies, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Yasman . Center for Natural Product Medicine Studies, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Zulfa Edawati Center for Natural Product Medicine Studies, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

DOI:

https://doi.org/10.22159/jap.2018.v10s1.17

Keywords:

1, 1-Diphenyl-2-picrylhydrazyl, Ascidiacea, Didemnum sp, Seribu Islands

Abstract

Objective: This study aimed to determine the antioxidant activity of samples of the ascidian Didemnum sp. collected from Seribu Islands, Jakarta.
Methods: Antioxidant activity was tested using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Didemnum sp. was extracted into methanol and
then fractionated using n-hexane, ethyl acetate, and water. Fractions with the highest antioxidant activity were further fractionated using accelerated
column chromatography.
Results: The concentration of sample that reduced the DPPH radical by 50% (IC50) in a methanol extract of Didemnum sp. was 105.10 μg/mL. The ethyl
acetate fraction had the highest antioxidant activity (IC50 of 90.804 μg/mL). The most active fraction obtained from accelerated column chromatography
fraction had an IC50 of 86.35 μg/mL. The compounds contained in the most active fractions were alkaloids, saponins, steroids/triterpenoids, and
glycosides.
Conclusion: The methanol extract of the ascidian Didemnum sp. exhibited antioxidant activity. Fractionation of the Didemnum sp. extract showed that
the ethyl acetate fraction had the highest antioxidant activity. Further, fractionation of the ethyl acetate fraction by accelerated column chromatography
showed that fraction VI had the highest antioxidant activity. The most active fraction contained alkaloids, steroids/triterpenoids, saponins, and
glycosides.

Downloads

Download data is not yet available.

References

Moosa MK. Marine Resources Nusantara: Marine Biodiversity and its

Preservation. Jakarta: LIPI; 1999.

Rasyid A. Marine Biota as a source of medicines. Oseana 2008;33:11-8.

Cahyana AH. Pyropheophytin-a as an antioxidative substance from the

marine algae (Eienia byciclis). J Biosa Biotech Biochem 1991;56:1533-5.

Kijjoa A, Sawangwong P. Drugs and cosmetics from the sea. Mar Drugs

;2:73-82.

Boer Y. Test the antioxidant activity of Garcinia parvifolia Miq.

J Matematika dan IPA 2000;1:26-33.

Sofia D. Antioxidants and Free Radicals; 2008. p. 37. Available from:

http://www.chem-is-try.org. [Last accessed on 2011 Sep 10].

Pratiwi DP, Harapini M. Peroxide value and anti-free radical activity

of diphenyl picril hydrazil hydrate methanol extract Knema laurina.

Majalah Farmasi Indones 2006;17:32-6.

Rohdiana D. Activity of capture power radical polyphenols in tea

leaves. Majalah J Indones 2001;12:53-8.

Sunarni T. Antioxidant activity free radical catcher some sprouts from

Papilionaceae Familia plant seed. J Farmasi Indones 2005;2:53-61.

Allen GR, Steene R. Tropical Reef Research. Singapura: Indo-Pacific

Coral Reef Field Guide; 2002. p. 378.

Cohen AN. Guide to the Exotic Species of San Francisco Bay; 2005.

p. 4. Available from: http://www.sfu.ca/bisc/bisc-842/michael/web_

page/antifeed.htm. [Last accessed on 2011 Aug 16].

Boyer M. Urn Ascidia. 2006; 16. Available from http://www.seadb.net/

en_Urn-ascidia-Didemnu. [Last accessed on 2011 Aug 16].

Krishnaiah P, Reddy VL, Venkataramana G, Ravinder K, Srinivasulu M,

Raju TV, et al. New lamellarin alkaloids from the Indian ascidian

Didemnum obscurum and their antioxidant properties. J Nat Prod

;67:1168-71.

Sasikumar JM, Maheshu V, Jayadev R. In vitro antioxidant activity of

methanolic extracts of Berberis tinctoria Lesch. Root and root bark,

India. J Herbal Med Toxycol 2009;3:53-8.

Blois MS. Antioxidant determinations by the use of a stable free radical.

Nature 1958;181:1199-2000.

Farnsworth NR. Biological and phitochemical screening of plants.

J Pharm Sci 1966;55:226-7.

Schupp P. Structure elucidation, biological activity and ecology of

secondary metabolites from Micronesian marine invertebrates. In:

Dissertation. Bayerischen Julius-Maximillians-Universität, Würzbürg).

Bandung: ITB;2000. p. 202.

Hanani E, Munim A, Sekarini R. Identification of antioxidant

compounds Spons callyspongia sp from Seribu Islands. Majalah Ilmu

Kefarmasian 2005;2:127-33.

Pokorni. Antioxidant in food. In: Practical Applications. New York:

CRC Press; 2001.

Mun’im A, Azizahwati T. The antioxidant activity of the pleurotaceae

and polyporaceae from universitas Indonesia’s forest. J Ilmiah Farmasi

;5:36-41.

Published

20-12-2018

How to Cite

Elya, B., ., Y., & Edawati, Z. (2018). ANTIOXIDANT ACTIVITY OF THE ASCIDIAN MARINE INVERTEBRATES, DIDEMNUM SP. International Journal of Applied Pharmaceutics, 10(1), 81–86. https://doi.org/10.22159/jap.2018.v10s1.17

Issue

Section

Original Article(s)