LIPOXYGENASE INHIBITORY ASSAY OF ETHYL ACETATE FRACTION FROM STAR FRUIT LEAVES (AVERRHOA CARAMBOLA L.) FROM THREE REGIONS IN WEST JAVA
DOI:
https://doi.org/10.22159/ijap.2020.v12s1.FF025Keywords:
Averrhoa carambola L, Inflammation, Lipoxygenase, Determination of flavonoid content, Determination of phenol contentAbstract
Objective: Averrhoa carambola L. leaves have been used as a traditional medicine. The study aimed to examine the anti-inflammatory activity of
A. carambola L. plant extracts using a lipoxygenase inhibition assay based on ethyl acetate fractions of A. carambola L. leaves obtained from three
regions in West Java.
Methods: Extracts from A. carambola L. leaves were obtained by maceration method using 70% ethanol followed by fractionation using liquid-liquid
partitioning until the ethyl acetate fraction was obtained. Subsequently, lipoxygenase inhibition activity of the ethyl acetate fraction was tested using
linoleate acid as the substrate, and the total flavonoid and total phenol contents were determined. The correlation between the IC50 values and the
total flavonoid and total phenol contents obtained from the ethyl acetate fraction were analyzed.
Results: The ethyl acetate fraction from the sample inhibited lipoxygenase enzyme activity with IC50 values of 19.38, 16.65, and 15.07 μg/mL, respectively.
The total flavonoid contents in the ethyl acetate fractions obtained from Depok, Subang, and Sukabumi regions were 14.88, 16.88, and 22.27 mg QE/g
of sample, respectively, whereas the total phenol contents were 54.10, 61.06, and 72.18 mg gallic acid equivalents/g of sample, respectively.
Conclusion: There was a high correlation between the IC50 values and the total flavonoid and total phenol contents, with correlations coefficients
of −0.917 and −0.960, respectively, which indicated that the higher the total flavonoid and total phenol contents, the lower the IC50 values.
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References
review. Int J Pharm Res Rev 2015;2:58.
2. Khanam Z, Sam KH, Hazerra N, Ching CH, Bhat IU. Determination
of polyphenolic content, HPLC analyses and DNA cleavage activity of
Malaysian Averrhoa carambola L. fruit extracts. J King Saud Univ Sci
2015;27:331-7.
3. Moresco HH, Queiroz GS, Pizzolatti MG, Brighente IM. Chemical
constituents and evaluation of the toxic and antioxidant activities of
Averrhoa carambola leaves. Rev Bras Farmacog 2012;22:319-24.
4. Murray RK, Granner DK, Rodwell VW. Harper’s Biochemical. 27th ed.
Jakarta: Buku Kedokteran EGC; 2009.
5. Avelia S, Mauldina MG, Elya B. Antioxidant activity and
lipoxygenase inhibitory assay with total flavonoid content of
Garcinia lateriflora blume leaves extract. Asian J Pharm Clin Res
2017;10:163-5.
6. Elya B, Puspitasari N, Sudarmin AC. Antioxidant activity and inhibition
of lipoxygenase activity ethanol extract of endosperm Arenga pinnata
(wurmb) merr. Asian J Pharm Clin Res 2017;10:76-80.
7. Thomas S, Patil D, Patil AG, Chandra N. Pharmacognostic evaluation
and physicochemical analysis of Averrhoa carambola L. fruit. J Herb
Med Toxicol 2008;2:51-4.
8. Nabilah, Elya B, Djajadisastra J. Lipoxygenase inhibitory assay
of Averrhoa carambola L. leaves extract. Int J ChemTech Res
2017;10:342-7.
9. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid
content in propolis by two complementary colorimetric method. J Food
Drug Anal 2002;10:178-82.
10. Ainsworth EA, Gillespie KM. Estimation of total phenolic content
and other oxidation substrates in plant tissues using Folin-Ciocalteu
reagent. Nat Protoc 2007;2:875-7.
11. Ministry of Health of the Republic of Indonesia. Supplements II,
Indonesian Herbal Pharmacopoeia. Jakarta: Ministry of Health of the
Republic of Indonesia; 2011.
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