DETECTION OF BISPHENOL A CONTAMINATION IN CANNED CARBONATED BEVERAGES BY GAS CHROMATOGRAPHY
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.24Keywords:
Bisphenol A, Gas chromatography, Contamination analysis, Validation, Canned beveragesAbstract
Objective: The purpose of this study was to develop sensitive, selective, and valid methods for the detection of bisphenol A (BPA) contamination in
beverage samples using gas chromatography (GC)-flame ionization.
Methods: The optimized analysis system employed a long HP-1 capillary column (30 m, inner diameter 0.25 mm, film thickness 0.25 μm), gradient
column temperature (150°C–260°C at 10°C/min), and nitrogen as a carrier gas (1 mL/min). Samples were prepared for analysis using ethyl acetate
as the extraction solvent.
Results: This method yielded a linearity coefficient of 0.9998, while the limit of detection (LOD) and limit of quantitation (LOQ) were 0.287 μg/mL and
0.956 μg/mL, respectively. All validation parameters, including linearity, selectivity, accuracy, precision, LOD, and LOQ, meet recognized acceptability
criteria. Contamination analysis showed that one of the three beverage brands tested contained 2.4090 μg/mL BPA, and contamination was even
higher after heating.
Conclusion: BPA contamination may occur in canned beverages, especially under improper storage conditions. This GC-based BPA detection system
may be useful for the detection of BPA contamination in consumer beverages.
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References
Ng HW, Shu M, Luo H, Ye H, Ge W, Perkins R, et al. Estrogenic activity
data extraction and in silico prediction show the endocrine disruption
potential of bisphenol A replacement compounds. Chem Res Toxicol
;28:1784-95.
Shrinithivihahshini N, Mahamuni D, Praveen N. Bisphenol a migration
study in baby feeding bottles of selected brands available in the Indian
market. Curr Sci 2014;106:1081-84.
European Food Safety Authority (EFSA). Opinion of the scientific
panel on food additives, flavourings, processing aids and materials in
contact with food (AFC) on request from the commission related to
,2 bis(hydroxyphenol)propane (bisphenol A). Eur Food Soc Authority
;428:1-75.
Errico S, Bianco M, Mita L, Migliaccio M, Rossi S, Nicolucci C,
et al. Migration of bisphenol A into canned tomatoes produced in
Italy: Dependence on temperature and storage conditions. Food Chem
;160:157-64.
Nagel R., Bosch-Domenech A, Satorra A, Garcia-Montalvo J. One, Two,
(Three), Infinity: NewspaperandLabBeauty-ContestExperiments. Barcelone:
Universitat Pompeu Fabra. Available from: http://www.citeseerx.ist.psu.
edu/viewdoc/download?doi=10.1.1.479.4035&rep=rep1&type=pdf.
Careri M, Furlattini L, Mangia A, Musc M, Anklam E, Theobald A, et al.
Supercritical fluid extraction for liquid chromatographic determination
of carotenoids in spirulina Pacifica algae: A chemometric approach.
J Chromatogr A 2001;912:61-71.
Inoue K, Mizuno T, Wada K, Hagiwara M. Novel RING finger proteins,
air1p and air2p, interact with hmt1p and inhibit the arginine methylation
of npl3p. J Biol Chem 2000;275:32793-9.
Sajiki H, Hirota K. Formation of Pd/C-ethylenediamine complexes
[Pd/C(en)] and its application to selective hydrogenations. scientific
magazine of synthesis 2001;59:109-20.
Pedersen B, Rasmussen KE. Liquid-liquid-liquid microextraction for
sample preparation of biological fluids prior to capillary electrophoresis.
Anal Chem 1999;71:2650-6.
Jeannot R, Sabik H, Sauvard E, Dagnac T, Dohrendorf K. Determination
of endocrine-disrupting compounds in environmental samples using
gas and liquid chromatography with mass spectrometry. J Chromatogr
A 2002;974:143-59.
Zafra G, Del Olmo M, Pulgar R, Navalon A, Vilchez JL. Determination
of bisphenol-A and related compounds in human saliva by gas
chromatography-mass spectrometry. Chromatographia 2002;56:213-8.
Harmita H, Mansur U, Stephanie S. Determination of docosahexaenoic
acid in infant formulas with gas chromatography. Asian J Pharm Clin
Res 2018;11:247-50.
Sujatha P, Evanjaline M, Muthukumarasamy S, Mohan VR.
Determination of bioactive components of Barleria courtallica Nees
(Acanthaceae) by gas chromatography–mass spectrometry analysis.
Asian J Pharm Clin Res 2017;10:273-83.
De Meulenaer B, Baert K, Lanckriet H, Van Hoed V, Huyghebaert A.
Development of an enzyme-linked immunosorbent assay for bisphenol A
using chicken immunoglobulins. J Agric Food Chem 2002;50:5273‑82.
Wang FC, Burleson D. The development of pyrolysis-fast gas
chromatography for analysis of synthetic plymers. J Chromatogr A
;833:111-9.
Chaskar PK, Tank SH, Doshi GM. Gas chromatography-mass
spectroscopy studies on Cestrum nocturnum macerated methanolic
extract. Asian J Pharm Clin Res 2017;10:259-63.
Rykowska I, Szymanski A, Waisak W. Determination of bisphenol A
in drinking water using new SPE sorbents with chemically bonded
ketamine group. Pol J Food Nutr Sci 2005;13:355-61.
Miryani A, Leilani G, Zenilda D. Hollow fiber liquid-phase
microextration-gas chromatography-mass spectrometry method to
analyze bisphenol A and other plasticizer metabolites. J Chromatogr A
;1481:31-6.
Szyrwinska K, Lulek J. Chemically bonded phases for the analysis of trace
amounts of organic pollutants. Toxicol Mech Methods 2008;18:537-42.
International Conference on Harmonisation (ICH). Guidance for
Industry: Validation of Analytical Procedures; 1996.
Cao XL, Corriveau J, Popovic S. Levels of bisphenol A in canned soft
drink products in Canadian markets. J Agric Food Chem 2009;57:1307‑11.