ANALYSIS OF BISPHENOL A IN INDONESIAN CANNED FOOD BY GAS CHROMATOGRAPHY
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.05Keywords:
Bisphenol A, Canned foods, Contamination analysis, Gas chromatography-flame ionization detection, ValidationAbstract
Objective: This study aimed to design and optimize a gas chromatography-flame ionization detection (GC-FID) method to determine the bisphenol A (BPA)
content in Indonesian canned food samples.
Methods: GC with Hewlett-Packard-1 capillary columns (length, 30 m; inside diameter, 0.25 mm; and film thickness, 0.25 μm) was used with a column
temperature of 150°C that was programmed to increase by 10°C/min to 260°C. Injector and detector temperatures were 280 and 300°C, respectively,
the gas flow rate was 1.0 mL/min, and injection volume was 3.0 μL. Three types of canned food samples were prepared by ethyl acetate extraction and
stored under four different conditions (4–8°C, 25–30°C, 40°C for 30 min, and 40°C for 60 min) to determine BPA migration levels.
Results: Method validation (system compatibility, selectivity, calibration curve linearity, accuracy, and precision) was acceptable for BPA
concentrations ranging from 2 to 15 μg/mL, with a coefficient of correlation of 0.99983. The limits of detection and quantitation were 0.287 and
0.956 μg/mL, respectively. Only one canned food sample type (Group A) showed BPA contamination under all storage conditions and exceeded the
recommended guidelines for daily ingestion.
Conclusion: The optimized GC-FID method was selective and relatively sensitive in the detection and quantitation of BPA. Furthermore, higher
storage temperatures and durations increased the level of BPA migration into food.
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