SYNTHESIS AND ANALYSIS OF ZINC METHIONINE, ZINC GLYCINE, COPPER LEUCINE, AND COPPER GLYCINE COMPLEXES USING ATOMIC ABSORPTION SPECTROPHOTOMETRY
Keywords:Amino acid complex, Atomic absorption spectrophotometry, Concentration, Metal
Objective: The aim of this study was to perform metal-amino acid synthesis and to analyze the free and bonded mineral concentrations.
Methods: In this study, the synthesis of amino acid metal complexes was carried out by reacting free metal ions, derived from a water-soluble metal
salt, with amino acids in a 1:2 molar ratio.
Results: The respective yields of this synthesis process were 95.38%, 95.95%, 76.31%, and 93.91% for zinc (Zn)-methionine (Zn(Met)2), Zn-glycine
(Zn(gli)2), copper-leucine (Cu(leu)2), and Cu-glycine (Cu(gli)2) complexes, respectively. The metal-amino acid complexes were then separated using
column chromatography and further analyzed by atomic absorption spectrophotometry (AAS). The bonded metal concentrations of the Zn(Met)2,
Zn(gli)2, Cu(leu)2, and Cu(gli)2 complexes were 189.32 mg/g, 353.78 mg/g, 180.89 mg/g, and 275.11 mg/g, respectively. The free metal concentrations
of the Zn(Met)2, Zn(gli)2, Cu(leu)2, and Cu(gli)2 complexes were 13.57 mg/g, 12.92 mg/g, 0.19 mg/g, and 2.12 mg/g, respectively.
Conclusion: In this study, Zn(Met)2, Zn(gli)2, Cu(leu)2, and Cu(gli)2 complexes were successfully formed and analyzed. The mineral concentration in
each complex differed depending on the type of mineral and ligand.
Soetan K, Oloaiya C, Oyewole O. The importance of mineral elements
for humans, domestic animals and plants: A review. Afr J Food Sci
Corbo MD, Lam J. Zinc deficiency and its management in the pediatric
population: A literature review and proposed etiologic classification.
J Am Acad Dermatol 2013;69:616-240.
Fredlund K, Isaksson M, Rossander-HulthÃ©n L, Almgren A, Sandberg AS.
Absorption of zinc and retention of calcium: Dose-dependent inhibition
by phytate. J Trace Elem Med Biol 2006;20:49-57.
Wang C, Li B, Wang B, Xie N. Degradation and antioxidant activities
of peptides and zinc-peptide complexes during in vitro gastrointestinal
digestion. Food Chem 2015;173:733-40.
Miquel E, Farre R. Effects and future trends of casein phosphopeptides
on zinc bioavailability. Trends Food Sci Technol 2007;18:139-43.
Pal DT, Gowda NK, Prasad CS, Amarnath R, Bharadwaj U, Suresh
Babu G, et al. Effect of copper- and zinc-methionine supplementation
on bioavailability, mineral status and tissue concentrations of copper
and zinc in ewes. J Trace Elem Med Biol 2010;24:89-94.
Tokalioglu S, Elci L, Kartal S. Speciation and determination of heavy
metals in lake waters by atomic absorption spectrometry after sorption
on amberlite XAD-16 resin. Anal Sci 2000;16:1169-74.
Ashmead H. The Roles of Amino Acid Chelates in Animal Nutrition.
USA: Noyes Publications; 1993.
Berestova T, Kuzina L, Amineva N, Faizrakhmanov I, Massalimov I,
Mustafin A. ATR-FTIR spectroscopic investigation of the cis- and
trans-bis-(Î±-amino acids) copper(II) complexed. J Mol Struct
Murtini ES, Hastuti R, Gunawan DP. The Effect of Destruction on the
Determination of Cu (II) Levels in Well Water, Sea Water and Chrome
Coating Wastewater Using AAS. Undergraduate Thesis, Semarang:
Ayaprakash R, Saroj KS, Hemalatha S, Easwaramoorthy D. Synthesis,
characterizatioon, quantitative structure-activity relationship, docking,
antibacterial activity, and brine shrimp lethal studies on L-phenylalanine
schiff bases. Asian J Pharm Clin Res 2016;9:203-8.
Mohanambal D, Arulantony S. Synthesis, spectral characterization
of schiff base complexes based on pyrimidine moiety with molecular
docking with biomolecules. Asian J Pharm Clin Res 2018;11:93-8.
Jalhan S, Jindal A, Hemraj GA. Synthesis, biological activities and
chemistry of thiadiazole derivatives and schiff bases. Asian J Pharm
Clin Res 2012;5:199-8.
Raimon G. Comparison of Wet and Dry Digestion Methods by Atomic
Absorption Spectrophotometry. National Analytical Chemistry
Workshop. Yogyakarta: Jaringan Kerjasama Kimia Analitik; 1993.