SIMULTANEOUS IDENTIFICATION AND QUANTIFICATION OF HYDROQUINONE, TRETINOIN AND BETAMETHASONE IN COSMETIC PRODUCTS BY ISOCRATIC REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Authors

  • baitha PALANGGATAN maggadani Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
  • harmita Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
  • yahdiana harahap Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
  • HANA LILI NATASYA HUTABALIAN Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia

DOI:

https://doi.org/10.22159/ijap.2019v11i3.32297

Keywords:

hydroquinone, tretinoin, betamethasone, whitening cream, HPLC reversed phase, optimization, validation

Abstract

Objective: The objective of this study was to obtain a simple and selective analysis method for determination of hydroquinone, tretinoin and betamethasone in whitening creams using reversed phase high-performance liquid chromatography (HPLC).

Methods: Reverse Phase HPLC was used for method development, validation studies, and sample analysis. The method was optimized by evaluating several parameters that affects extraction of the sample, composition and types of mobile phase and also flow rate. Chromatographic separation was optimized on a C18 column [Sunfire, 250 x 4.6 mm, 5µm] utilizing a mobile phase consisting acetonitrile, methanol (90:10 v/v) and slightly addition of glacial acetic acid to reach pH 5in the ratio of 30: 50:20 v/v at a flow rate of 0.8 ml/min with UV detection at 270 nm and 350 nm.

Results: The analytical methods fulfilled the validation requirements including accuracy, precision, linearity, selectivity, detection limits, and quantitation limits. The results showed the mean levels of hydroquinone, tretinoin and betamethasone in samples A and B were 1.78%; 0.07%; 0.12% and 2.00%; 0.07%; 0.13% respectively.

Conclusion: The method was successfully applied for the determination of cosmetic formulation containing hydroquinone, tretinoin and betamethasone simultaneously. There were seven samples analyzed and two samples were positive containing hydroquinone, tretinoin, and betamethasone.

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Author Biography

yahdiana harahap, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia

lecturer in faculty of pharmacy universitas indonesia

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Published

07-05-2019

How to Cite

maggadani, baitha P., harmita, harahap, yahdiana, & HUTABALIAN, H. L. N. (2019). SIMULTANEOUS IDENTIFICATION AND QUANTIFICATION OF HYDROQUINONE, TRETINOIN AND BETAMETHASONE IN COSMETIC PRODUCTS BY ISOCRATIC REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. International Journal of Applied Pharmaceutics, 11(3), 181–185. https://doi.org/10.22159/ijap.2019v11i3.32297

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