• FEBRINA AMELIA SAPUTRI Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
  • PATIHUL HUSNI Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia
  • NORISCA ALIZA PUTRIANA Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia



Azadirachtin, Chemical stability, High-performance liquid chromatography, Neem oil cream, Physical stability


Objective: This study aims to examine the physical and chemical stability of neem oil cream.

Methods: Physical stability was conducted by storing the cream at room temperature (25±2 °C/65 %RH±5 %RH) and high temperature (40±2 °C/75 % RH±5 % RH) for 3 mo. HPLC method using Dionex with UV detection at 219 nm, Shodex (C-18) HPLC packed column (4.6 mmID x 250 mmL), acetonitrile: water [30:70] as mobile phase, 10 min isocratic elution with a flow rate of 1.0 ml/min with volume injection 20 μL was validated then was carried out to measure azadirachtin levels in neem oil cream. The chemical stability of azadirachtin in the cream was determined for 90 d by using this validated method.

Results: The neem oil cream was physically stable. The HPLC method of azadirachtin meets all the validation parameters and can be used to analyze the chemical stability of azadirachtin in neem oil cream. Neem oil cream was stable for 4 w at 25 °C and for 1 w at 40 °C.

Conclusion: The neem oil cream was either physically or chemically stable for 4 w at 25 oC and 1 w at 40 oC


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