IN VITRO POTENCY AND TOXICITY OF STREPTOMYCES SP. FERMENTATION PRODUCT AS AN ANTIMALARIAL THERAPY AGAINST PLASMODIUM FALCIPARUM
DOI:
https://doi.org/10.22159/ijap.2019.v11s1.16Keywords:
Antimalarial, Streptomyces sp, Fermentation Product, Half maximal inhibitory concentration, Transmission electron microscopy, ToxicityAbstract
Objective: This research aims to study the activity of a Streptomyces sp. fermentation product as an antimalarial modality in HepG2 cells.
Methods: The effects of the product against Plasmodium falciparum 3D7 were examined using an in vitro technique parasite. The potency of the
Streptomyces sp. fermentation product was examined by determining the half maximal inhibitory concentration (IC50), and the mechanism was
studied using transmission electron microscopy (TEM). Toxicity tests were also conducted.
Results: The Streptomyces sp. fermentation product had an IC50 of 0.001 μg/ml against the parasite, versus values of 0.054 and 0.022 μg/ml for
quinidine and prodigiosin, respectively. TEM revealed no formation of hemozoin. The Streptomyces sp. fermentation product was non-toxic in HepG2
cells based on its cytotoxicity concentration 50% of 1.380 μg/ml.
Conclusion: The Streptomyces sp. fermentation product has potential as a potent and non-toxic antimalarial therapy.
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selected Streptomyces species. Bioorg Med Chem Lett 2015;25:5646-9.
25. Chaturvedi D, Goswami A, Saikia PP, Barua NC, Rao PG. Artemisinin
and its derivatives: A novel class of anti-malarial and anti-cancer
agents. Chem Soc Rev 2010;39:435-54.
26. de Araújo HW, Fukushima K, Takaki GM. Prodigiosin production by
Serratia marcescens UCP 1549 using renewable-resources as a low
cost substrate. Molecules 2010;15:6931-40.
27. Intaraudom C, Bunbamrung N, Dramae A, Danwisetkanjana K,
Rachtawee P, Pittayakhajonwut P. Antimalarial and antimycobacterial
agents from Streptomyces Sp. Tetrahedron Lett 2015;56:6875-7.
28. Intaraudom C, Rachtawee P, Suvannakad R, Pittayakhajonwut P.
Antimalarial and antituberculosis substances from Streptomyces Sp.
BCC26924. Tetrahedron 2011;67:7593-7.
29. Boukandou Mounanga M, Mewono L, Aboughe Angone S.
Toxicity studies of medicinal plants used in Sub-Saharan Africa. J
Ethnopharmacol 2015;174:618-27.
drug resistance: Literature review and activities and findings of the
ICEMR network. Am J Trop Med Hyg 2015;93:57-68.
2. Indonesian Ministry of Health. Control of Drug and Insecticide
Resistance. Malaria Management Guidelines; 2014. p. 129-38.
3. World Health Organization. WHO Global Malaria Programme.
World Malaria Report 2015. Available from: https://www.apps.who.
int/iris/bitstream/handle/10665/200018/9789241565158_eng.pdf;
jsessionid=5859DB1FF50D46B863C585A70A6DCE13?sequence=1.
[Last accessed on 2016 Jan 28].
4. Petersen I, Eastman R, Lanzer M. Drug-resistant malaria: Molecular
mechanisms and implications for public health. FEBS Lett
2011;585:1551-62.
5. Meshnick SR. Artemisinin: Mechanisms of action, resistance and
toxicity. Int J Parasitol 2002;32:1655-60.
6. Saxena S, Pant N, Jain DC, Bhakuni RS. Antimalarial agents from plant
sources. Curr Sci 2003;85:1314-29.
7. Mojab F. Antimalarial natural products: A review. Avicenna J Phytomed
2012;2:52-62.
8. Baba MS, Zin NM, Hassan ZA, Latip J, Pethick F, Hunter IS,
et al. In vivo antimalarial activity of the endophytic actinobacteria,
streptomyces SUK 10. J Microbiol 2015;53:847-55.
9. Boonlarppradab C, Suriyachadkun C, Rachtawee P, Choowong W.
Saccharosporones A, B and C, cytotoxic antimalarial angucyclinones
from Saccharopolyspora Sp. BCC 21906. J Antibiot (Tokyo)
2013;66:305-9.
10. Bérdy J. Bioactive microbial metabolites. J Antibiot (Tokyo) 2005;
58:1?26.
11. Procópio RE, Silva IR, Martins MK, Azevedo JL, Araújo JM. Antibiotics
produced by streptomyces. Braz J Infect Dis 2012;16:466?71.
12. Isaka M, Jaturapat A, Kramyu J, Tanticharoen M, Thebtaranonth Y.
Potent in vitro antimalarial activity of metacycloprodigiosin isolated
from Streptomyces spectabilis BCC 4785. Antimicrob Agents
Chemother 2002;46:1112-3.
13. Manteca A, Sanchez J. Streptomyces development in colonies and
soils. Appl Environ Microbiol 2009;75:2920-4.
14. Bibb MJ. Regulation of secondary metabolism in streptomycetes. Curr
Opin Microbiol 2005;8:208-15.
15. El-bondkly AM. Overproduction and biological activity of prodigiosinlike
pigments from recombinant fusant of endophytic marine
Streptomyces species. Antonie van Leeuwenhoek 2012;102:719-34.
16. Soliev AB, Hosokawa K, Enomoto K. Bioactive pigments from marine
bacteria: Applications and physiological roles. Evid Based Complement
Alternat Med 2011;2011:670349.
17. Lins LJ, Maciel CC, Xavier HS, da Silva CA, Campos-Takaki GM.
Production and toxicological evaluation of prodigiosin from Serratia
marcescens UCP/WFCC1549 on mannitol solid medium. Int J Appl
Res Nat Prod 2014;7:32-8.
18. Papireddy K, Smilkstein M, Kelly JX, Shweta, Salem SM,
Alhamadsheh M, et al. Antimalarial activity of natural and synthetic
prodiginines. J Med Chem 2011;54:5296-306.
19. Lazaro JE, Nitcheu J, Predicala RZ, Mangalindan GC, Nesslany F,
Marzin D, et al. Heptyl prodigiosin, a bacterial metabolite, is antimalarial
in vivo and non-mutagenic in vitro. J Nat Toxins 2002;11:367-77.
20. Rahul S, Chandrashekhar P, Hemant B, Bipinchandra S, Mouray E,
Grellier P, et al. In vitro antiparasitic activity of microbial pigments and
their combination with phytosynthesized metal nanoparticles. Parasitol
Int 2015;64:353-6.
21. Stankovic N, Senerovic L, Ilic-Tomic T, Vasiljevic B, Nikodinovic-
Runic J. Properties and applications of undecylprodigiosin and other
bacterial prodigiosins. Appl Microbiol Biotechnol 2014;98:3841-58.
22. Winey M, Meehl JB, O’Toole ET, Giddings TH Jr. Conventional
transmission electron microscopy. Mol Biol Cell 2014;25:319-23.
23. Wilkening S, Stahl F, Bader A. Comparison of primary human
hepatocytes and hepatoma cell line hepg2 with regard to their
biotransformation properties. Drug Metab Dispos 2003;31:1035-42.
24. Rakotondraibe LH, Rasolomampianina R, Park HY, Li J, Slebodnik C,
Brodie PJ, et al. Antiproliferative and antiplasmodial compounds from
selected Streptomyces species. Bioorg Med Chem Lett 2015;25:5646-9.
25. Chaturvedi D, Goswami A, Saikia PP, Barua NC, Rao PG. Artemisinin
and its derivatives: A novel class of anti-malarial and anti-cancer
agents. Chem Soc Rev 2010;39:435-54.
26. de Araújo HW, Fukushima K, Takaki GM. Prodigiosin production by
Serratia marcescens UCP 1549 using renewable-resources as a low
cost substrate. Molecules 2010;15:6931-40.
27. Intaraudom C, Bunbamrung N, Dramae A, Danwisetkanjana K,
Rachtawee P, Pittayakhajonwut P. Antimalarial and antimycobacterial
agents from Streptomyces Sp. Tetrahedron Lett 2015;56:6875-7.
28. Intaraudom C, Rachtawee P, Suvannakad R, Pittayakhajonwut P.
Antimalarial and antituberculosis substances from Streptomyces Sp.
BCC26924. Tetrahedron 2011;67:7593-7.
29. Boukandou Mounanga M, Mewono L, Aboughe Angone S.
Toxicity studies of medicinal plants used in Sub-Saharan Africa. J
Ethnopharmacol 2015;174:618-27.
Published
05-04-2019
How to Cite
SETYANINGSIH, Y., LATIF, A., ASTUTY, H., SYAFRUDDIN, D., & ASIH, P. B. S. (2019). IN VITRO POTENCY AND TOXICITY OF STREPTOMYCES SP. FERMENTATION PRODUCT AS AN ANTIMALARIAL THERAPY AGAINST PLASMODIUM FALCIPARUM. International Journal of Applied Pharmaceutics, 11(1), 251–255. https://doi.org/10.22159/ijap.2019.v11s1.16
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