CHEMICAL PROFILE OF TWO JASMINUM SAMBAC L. (AIT) CULTIVARS CULTIVATED IN EGYPT–THEIR MEDIATED SILVER NANOPARTICLES SYNTHESIS AND SELECTIVE CYTOTOXICITY

SEHAM S.  EL-HAWARY; HALA M. EL-HEFNAWY; SAMIR M. OSMAN; EMAN S. MOSTAFA; FATMA ALZAHRAA MOKHTAR; MOHAMED A.  EL-RAEY

doi:10.22159/ijap.2019v11i6.33646

Authors

SEHAM S. EL-HAWARY Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
HALA M. EL-HEFNAWY Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
SAMIR M. OSMAN Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
EMAN S. MOSTAFA Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 6th October City, Giza, Egypt
FATMA ALZAHRAA MOKHTAR Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
MOHAMED A. EL-RAEY Phytochemistry and Plant Systematic Department, National Research Centre, Dokki, Cairo, Egypt

DOI:

https://doi.org/10.22159/ijap.2019v11i6.33646

Keywords:

Jasminum sambac, AgNPs, HPLC-PDA-MSMS, Cytotoxicity, Green synthesis

Abstract

Objective: Evaluation of two Jasminum sambac L. (Ait) cultivars; Arabian Nights (JSA) and Grand Duke of Tuscany (JSG) ethanolic leaves extracts as reducing agents for the green synthesis of silver nanoparticles (AgNPs) and evaluation of their cytotoxicity against MCF-7 breast cancer and 5637 bladder cancer cell lines and chemical profiling of the two cultivars.

Methods: The synthesis of silver nanoparticles (AgNPs) by the two cultivars and characterization of AgNPs by ultraviolet (UV)–visible spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR). Additionally, the use of The high-performance liquid chromatography coupled with photodiode array-mass-mass-spectroscopy (HPLC-PDA-MS/MS) for chemical profiling of both cultivars and evaluation of total leaves extracts and corresponding nanoparticles towards MCF-7 and 5637 cell lines compared to aneuploidy immortal keratinocyte (Ha Cat) normal cells by neutral cell assay.

Results: The green synthesized AgNPs (of an average size range of 8.83 and 11.24 nm for JSA and JSG, respectively) exhibited cytotoxicity against MCF-7 and 5637 cell lines. The IC₅₀ was determined for each total extract JSA (15.29±2.16 μg/ml) and JSG (20.28±1.20 μg/ml) and corresponding AgNPs 17.32±2.22 μg/ml and 6.32±1.01μg/ml for JSA and JSG, respectively. The IC₅₀ of JSA and JSG against 5637 bladder cancer cell line were 13.76±1.11 μg/ml and 50.69±3.75 μg/ml, while the corresponding AgNPs showed IC₅₀ of 5.54±0.88 μg/ml and 27.89±2.84 μg/ml, respectively. The HPLC-PDA-MS/MS allowed the identification of 59 compounds; 10 simple phenols, 17 flavonoids; quercetin and kaempferol glycosides, 2 lignans, and 30 secoiridoids; oleuropein, molihauside, and sambacoside.

Conclusion: This study proved that JSA is an excellent source for the synthesis of AgNPs with optimum characters and enhanced activities toward MCF-7 and 5637 cell lines in correlation to identified compounds.

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