PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE SP.) AND HATI TANAH (ANGIOPTERIS SP.) AND THEIR COMBINATION AGAINST PROPIONIBACTERIUM ACNES
DOI:
https://doi.org/10.22159/ijap.2019.v11s3.M0011Keywords:
Antibacterial activity, Eleutherine sp, Angiopteris sp, Propionibacterium acnesAbstract
Objective: The objective of this research was to investigate the preliminary phytochemical screening and antibacterial activity of Bawang Dayak
(Eleutherine sp.) and Hati Tanah (Angiopteris sp.) and their combination against Propionibacterium acnes.
Methods: The extracts were used for phytochemical screening. Antibacterial activity was performed using disc diffusion technique, with two
variations of the concentration of 5% and 10% for each extracts, and combination of both extracts with three combinations: (1) 5%: 5%, (2) 5%: 10%, and (3) 10%: 5%.
Results: Both extracts contained tannins, saponins, and steroids. The antibacterial activity against P. acnes showed that the inhibition zones of
Bawang Dayak ethanol extract were 6.1±1.5 mm (5%) and 8.7±1.3 mm (10%). On the other hand, the inhibition zones of Hati Tanah ethanol extract were 4.0±1.6 mm (5%) and 9.2±2.5 mm (10%). The inhibition zones produced in combinations 1, 2, and 3 were 5.8±0.3 mm, 10.8±2.0 mm, and 15.5±2.8 mm, respectively.
Conclusion: In this study showed the presence of tannins, saponins, and steroids that might be responsible for antibacterial activity in both extract. The best antibacterial activity was produced by combining the two extracts (combination 3).
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2. Toyoda M, Morohashi M. Pathogenesis of acne. Med Electron Microsc 2001;34:29-40.
3. Coenye T, Peeters E, Nelis HJ. Biofilm formation by Propionibacterium acnes is associated with increased resistance to antimicrobial agents and increased production of putative virulence factors. Res Microbiol 2007;158:386-92.
4. Webster GF. Acne vulgaris. BMJ 2002;325:475-9.
5. Margolis DJ, Bowe WP, Hoffstad O, Berlin JA. Antibiotic treatment of acne may be associated with upper respiratory tract infections. Arch Dermatol 2005;141:1132-6.
6. Krismawati A, Sabran M. Genetic Resource Management of Specific Medicinal Plants of Central Kalimantan. Palangka Raya: Balai Pengkajian Teknologi Pertanian Kalimantan Tengah; 2006.
7. Subeki, Matsuura H, Yamasaki M, Yamato O, Maede Y, Katakura K, et al. Effects of central kalimantan plant extracts on intraerythrocytic babesia gibsoni in culture. J Vet Med Sci 2004;66:871-4.
8. Galingging RY. Bawang dayak as multifunctional medicinal plants. Res Dev News 2009;15:2-4.
9. Mierza V, Suryanto D, Nasution PM. Phytochemical Screening and Antibacterial Effect Assay of Bawang Sabrang (Eleutherine palmifolia Merr.) Bulbus Ethanol Extract. Proceeding of National Seminars; 2011.
10. Warnida H, Sukawaty Y, Mega. Stability and activity of bawang tiwai (Eleutherine americana (Mill.) Urb.) Bulbus gel extract as anti acne. J Ilmiah Manuntung 2015;1:94-9.
11. Handayani R, Novaryatiin S, Valentiningtyas WA. Identification of pharmacognostic hati tanah plant of origin of Palangka Raya, central Kalimantan. J Surya Med 2015;1:53-61.
12. Novaryatiin S, Handayani R, Chairunnisa R. Inhibitory assay of hati tanah (Angiopteris Sp.) bulbus ethanol extract against Staphylococcus aureus. J Surya Med 2017;3:23-31.
13. Kamatou GP, Viljoen AM, van Vuuren SF, van Zyl RL. In vitro evidence of antimicrobial synergy between Salvia chamelaegana and Leonotis leonurus. South Afr J Bot 2006;72:634-6.
14. Aiyegoro O, Adewusi A, Oyedemi S, Akinpelu D, Okoh A. Interactions of antibiotics and methanolic crude extracts of Afzelia africana (Smith.) against drug resistance bacterial isolates. Int J Mol Sci 2011;12:4477-503.
15. Manigandan M, Kolanjinathan K. Qualitative phytochemical screening and antioxidant activity of Elytraria acaulis Lindau (Acanthaceae). Asian J Pharm Clin Res 2016;9:1-4.
16. Azis T, Febrizky S, Mario AD. The effect of type of solvents to alkaloids yield percent from salam India (Murraya koenigii) Leaves. Teknik Kimia 2014;20:1-6.
17. Pratiwi D, Wardaningsih S, Isnindar I. The test of antioxidant activity from bawang mekah leaves (Eleutherine americana Merr.) Using DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Tradit Med J 2013;18:9-16.
18. Mojab F, Kamalinejad M, Ghaderi N, Vahidipour HR. Phytochemical screening of some species of Iranian plants. Iran J Pharm Res 2003;2:77-82.
19. Ghosal M, Mandal P. Phytochemical screening and antioxidant activities of two selected ‘BIHI’ fruits used as vegetables in Darjeeling himalaya. Int J Pharm Pharm Sci 2012;4:567-74.
20. Bauer AW, Kirby WM, Sherirs JC, Turck M. Antibiotic susceptibility testing by standard single disk method. Am J Clin Pathol 1966;45:433-96.
21. Koneman WE, Allen DS, Janda MW, Scherchenberger CP, Winn WC. Antimicrobial susceptibility testing. In: Color Atlas and Text Book of Diagnostic Microbiology. 4th ed. Philadelphia, PA: JB Lippincott Company; 1992. p. 624, 629, 637.
22. Shimada T. Salivary proteins as a defense against dietary tannins. J Chem Ecol 2006;32:1149-63.
23. Zablotowicz RM, Hoagland RE, Wagner SC. Effect of saponins on the growth and activity of rhizosphere bacteria. Adv Exp Med Biol 1996;405:83-95.
24. Ravi L, Manasvi V, Praveena LB. Antibacterial and antioxidant activity of saponin from Abutilon indicum Leaves. Asian J Pharm Clin Res 2016;9:344-7.
25. Epand RF, Savage PB, Epand RM. Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins). Biochim Biophys Acta 2007;1768:2500-9.
26. Eady AE, Cove JH, Layton AM. Is antibiotic resistance in cutaneous propionibacteria clinically relevant? implications of resistance for acne patients and prescribers. Am J Clin Dermatol 2003;4:813-31.
Published
15-07-2019
How to Cite
NOVARYATIIN, S. (2019). PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE SP.) AND HATI TANAH (ANGIOPTERIS SP.) AND THEIR COMBINATION AGAINST PROPIONIBACTERIUM ACNES. International Journal of Applied Pharmaceutics, 11(3), 11–13. https://doi.org/10.22159/ijap.2019.v11s3.M0011
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