THE ANTI-INFLAMMATORY ACTIVITY OF ESSENTIAL OIL OF CLOVE (Syzygium aromaticum) IN ABSORPTION BASE OINTMENT WITH ADDITION OF OLEIC ACID AND PROPYLENE GLYCOL AS ENHANCER
DOI:
https://doi.org/10.22159/ijap.2019.v11s5.T0081Keywords:
Absorption base, Anti-inflammatory, Enhancer, Essential oil of cloveAbstract
Background : The optimal concentration of essential oil of clove in absorption base ointment as anti-inflammatory has been studied. The development
of formulations can be done by adding oleic acid and propylene glycol as enhancers. The purpose of this study was to determine the anti-inflammatory
activity of the essential oil of clove in absorption base ointment formula by adding a mixture of oleic acid and propylene glycol as enhancers.
Methods: In this study, the composition of oleic acid and propylene glycol was 100% oleic acid (FI), 50% oleic acid and propylene glycol (FII), and
100% propylene glycol (FIII). The profile of the anti-inflammatory activity essential oil of clove was carried out using male of mice Balb/C strain which
was induced inflammatory with croton oil on back of skin. After treatment, it was sacrificed and then was taken the back of skin to get histopathological
preparation. After that, the epidermal thickness, number of inflammatory cells, and cyclooxygenase (COX)-2 expression can be measured.
Results : Based on the results of the test, it shows that FIII has the smallest of the amount of COX-2 expression, the number of inflammatory cells, and
the epidermal thickness so the addition of the composition enhancer provides good anti-inflammatory activity.
Conclusion: The increasing concentration of propylene glycol caused the raising activity of essential oil of clove as anti-inflammatory.
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References
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activity of Syzigium aromaticum volatile oil in lotion with variation
composition of oleic acid and propylene glycol as enhancer. Tradit Med
J 2017;22:111-5.
23. Rahmawati D, Sugihartini N, Yuwono T. Anti-inflammatory activity of
ointment in water soluble base of volatile oil of Syzygium aromaticum
with variation composition of oleic acid and propylene glycol as
enhancer. Period Dermatol Venerol 2017;29:182-7.
24. Duracher L, Blasco L, Hubaud JC, Vian L, Marti-Mestres G. The
influence of alcohol, propylene glycol and 1,2-pentanediol on the
permeability of hydrophilic model drug through excised pig skin. Int J
Pharm 2009;374:39-45.
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HPMC dan NaCMC sebagai Antiinflamasi Lokal Pada Penyakit
Periodontal. Skripsi. Jakarta: UIN Syarif Hidayatullah; 2014.
26. Lane ME. Skin penetration enhancers. Int J Pharm 2013;447:12-21.
27. Mohammed D, Hirata K, Hadgraft J, Lane M. Influence of skin
penetration enhancers on skin barrier function and skin protease
activity. Eur J Pharm Sci 2014;51:118-22.
28. Remon JP. Absorption Enhancers. In: Swarbick J, editor. Encyclopedia
of Pharmaceutical Technology. 3rd ed. New York: Informa; 2007.
29. Santos P, Watkonson AC, Hadgraft J, Lane ME. Influence of penetration
enhancer on drug permeation from volatile formulations. Int J Pharm
2012;439:260-8.
activity of Syzygium aromaticum silver nanoparticles: In
vitro and in silico study. Asian J Pharm Clin Res 2017;10:370-3.
2. Sukandar D, Radiastuti N, Khoeriyah K. Karakterisasi senyawa aktif
anti bakteri minyak atsiri bunga cengkeh (Syzygium aromaticum L.).
J Kimia Terapan Indonesia 2010;12:4.
3. Chainy GB, Manna SK, Chaturvedi MM, Aggarwal BB. Anethole
blocks both early and late cellular responses transduced by tumor
necrosis factor: Effect on NFkappaB, AP-1, JNK, MAPKK and
apoptosis. Oncogene 2000;19:2943-50.
4. Ma Q, Kineer K. Chemoprotection by phenolic antioxidants, inhibition
of tumor necrosis factor alpha induction in macrophages. J Biol Chem
2002;277:2477-484.
5. Murakami Y, Shoji M, Hanazawa S, Tanaka S, Fujisawa S. Preventive
effect of bis-eugenol, a eugenol ortho dimer, on lipopolysaccharidestimulated
nuclear factor kappaB activation and inflammatory cytokine
axpression in macrophages. Biochem Pharmacol 2003;66:1061-6.
6. Sugihartini N, Haque AF, Yuwono T. Anti-inflammatory activity of
cream type O/W with concentration variation essential oils of clove
(Syzigium aromaticum). Adv Sci Lett 2017;23:12515-7.
7. Kresnanto VA, Sugihartini N, Yuwono T. Physical Properties and
Irritation Index Essential Oil of Clove (Szyzigium aromaticum)
in Absoprtion base Ointment with Variation Concentration. AIP
Conference Proceeding, 2016;1746:020011.
8. Latifah F, Sugihartini N, Yuwono T. Evaluation of physical properties
and irritation index of lotion containing Syzigium aromaticum clove
essential oil at various concentration. Tradit Med J 2016;21:1-5.
9. Safriani R, Sugihartini N, Yuliani S. Physical characteristic and
irritation index of Syzigium aromaticum essential oil in O/W and W/O
creams. IOP Conf Ser Mater Sci Eng 2005;259:1-6.
10. Sugihartini N, Lestari G, Yuliani S. Anti-inflammatory activity of
essential oil of clove (Syzygium aromaticum) in O/W and W/O creams.
Pharmaciana 2019;9:109-18.
11. Kumar VS, Niranjan SK, Irchhaiya R, Neeraj K, Ali A. A novel
transdermal drug delivery system. Int Res J Pharm 2012;3:39-44.
12. Sugihartini N, Yuwono T, Sovia V. Optimasi Formulasi Minyak Atsiri
Bunga Cengkeh (Syzygium aromaticum) Sebagai Sediaan Herbal
Terstandar Antiinflamasi,” Laporan Hibah Penelitian Tim Pascasarjana.
Yogyakarta: Universitas Ahmad Dahlan, 2015.
13. Sugihartini N. “Optimasi Komposisi Enhancer dan Emulgator pada
Formulasi Salep basis serap Fraksi Etil Asestat Ekstrak Teh Hijau
(Camellia sinensis, L) sebagai Sediaan Topikal Anti Inflamasi,”
Disertasi. Yogyakarta: Program Pascasarjana Universitas Gadjah Mada,
2013.
14 Orra S, Waltzman JT, Mlynek K, Duraes EF, Kundu N,
Zins JE. Periorbital phenol-croton oil chemical peel in conjunction
with blepharoplasty: An evolving technique for periorbital facial
rejuvenation. Plast Reconstr Surg 2015;136 Suppl 4:99-100.
15. Boligou AA, Moreira LR, Piana M, Campos MM, Oleivera SM.
Topical anti edematogenic and anti-inflammatory effect of Scutia
buxifolia reissek gel and stability study. J. Photochem Photobiol B
2017;167:29?s35.
16. Subramanian V, Vellaichamy E. Atrial natriuretik peptide (ANP)
inhibiting DMBA/croton oil induced skin tumor growth by modulating
NF-?B, MMPs and infiltrating Mast cells in swis albino mice. Eur J
Pharm 2014;740:388-97.
17. Zaouami M, Bitam A, Baz A, Benali Y, Ben-Mahdi MH. In vivo
evaluation of wound healing and anti-inflammatory activity of
methanolic extract of roots of Centaurea africana (L.) in topical
formulation. Asian J Pharm Clin Res 2017;10:341-6.
18. Goh CF, Lane ME. Formulation of diclofenac for transdermal delivery.
Int J Pharm 2014;473:607-16.
19. Zilfener JL, Leal S, Fournier PE. Non-steroidal anti-inflammatory
drugs for athletes: An update. Annu Phys Rehabil Med 2010;53:278-88.
20. Bhowmik D, Gopinath H, Pragati B, Duraivel S, Sampath KP. The
pharma innovation: Recent advances in novel topical drug delivery
system. Pharma J 2012;1:12-31.
21. Nikoni V, Ostadhadi S, Baktiarian A, Abbasi-Gonjani E, Habibian-
Dehkordi S, Rezaei-Rosham M, et al. The anti-inflammatory
and antipyretic effects of clove oil in healthy dogs after surgery.
Pharmanutrition 2017;5:52-7.
22. Iriani FA, Sugihartini N, Yuwono T. The profile of anti-inflammatory
activity of Syzigium aromaticum volatile oil in lotion with variation
composition of oleic acid and propylene glycol as enhancer. Tradit Med
J 2017;22:111-5.
23. Rahmawati D, Sugihartini N, Yuwono T. Anti-inflammatory activity of
ointment in water soluble base of volatile oil of Syzygium aromaticum
with variation composition of oleic acid and propylene glycol as
enhancer. Period Dermatol Venerol 2017;29:182-7.
24. Duracher L, Blasco L, Hubaud JC, Vian L, Marti-Mestres G. The
influence of alcohol, propylene glycol and 1,2-pentanediol on the
permeability of hydrophilic model drug through excised pig skin. Int J
Pharm 2009;374:39-45.
25. Ginting D. Formulasi Patch Natrium Diklofenak Berbasis Polimer
HPMC dan NaCMC sebagai Antiinflamasi Lokal Pada Penyakit
Periodontal. Skripsi. Jakarta: UIN Syarif Hidayatullah; 2014.
26. Lane ME. Skin penetration enhancers. Int J Pharm 2013;447:12-21.
27. Mohammed D, Hirata K, Hadgraft J, Lane M. Influence of skin
penetration enhancers on skin barrier function and skin protease
activity. Eur J Pharm Sci 2014;51:118-22.
28. Remon JP. Absorption Enhancers. In: Swarbick J, editor. Encyclopedia
of Pharmaceutical Technology. 3rd ed. New York: Informa; 2007.
29. Santos P, Watkonson AC, Hadgraft J, Lane ME. Influence of penetration
enhancer on drug permeation from volatile formulations. Int J Pharm
2012;439:260-8.
Published
15-09-2019
How to Cite
SUGIHARTINI, N., PRABANDARI, R., YUWONO, T., & RAHMAWATI, D. R. (2019). THE ANTI-INFLAMMATORY ACTIVITY OF ESSENTIAL OIL OF CLOVE (Syzygium aromaticum) IN ABSORPTION BASE OINTMENT WITH ADDITION OF OLEIC ACID AND PROPYLENE GLYCOL AS ENHANCER. International Journal of Applied Pharmaceutics, 11(5), 106–109. https://doi.org/10.22159/ijap.2019.v11s5.T0081
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