ANTIDIABETIC ACTIVITY OF NANOPARTICLES CONTAINING JAVANESE TURMERIC RHIZOME EXTRACT: THE STRATEGY TO CHANGE PARTICLE SIZE

DENI RAHMAT; YUNAHARA FARIDA; AVILLA TAN BRYLIANTO; ROS SUMARNY; SHIRLY KUMALA

doi:10.22159/jap.2020v12i4.36249

Authors

DENI RAHMAT Faculty of Pharmacy, Pancasila University, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia
YUNAHARA FARIDA Faculty of Pharmacy University of Pancasila, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia
AVILLA TAN BRYLIANTO Faculty of Pharmacy University of Pancasila, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia
ROS SUMARNY Faculty of Pharmacy University of Pancasila, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia
SHIRLY KUMALA Faculty of Pharmacy University of Pancasila, Srengseng Sawah, Jagakarsa, Jakarta Selatan 12640, Indonesia

DOI:

https://doi.org/10.22159/jap.2020v12i4.36249

Keywords:

Javanese turmeric rhizome, Curcumin, Extract, Nanoparticles, Antidiabetic

Abstract

Objective: The aim of this study was to investigate the antidiabetic activity of Javanese turmeric rhizome extract and its nanoparticles. Javanese turmeric rhizome contains curcumin, flavonoid, and xanthorrhizol that show the antidiabetic activity. The plant extracts entrapped within the nanoparticles have been reported to increase the active compound bioavailability. Thereby, the nanoparticles can improve the biological activity of active compounds.

Methods: The rhizome was extracted by kinetic maceration using 96% (v/v) ethanol, whereas the nanoparticles were prepared by the ionic gelation method with different formulations to obtain two types of nanoparticles (F1 and F2). The resulting nanoparticles were evaluated for their particle size, zeta potential, and morphology. The antidiabetic study was performed in a model of alloxan-induced diabetic mice. The mice were divided into five groups, namely normal, negative, positive, extract (dose of 400 mg/kg) and nanoparticles group (dose of 400 mg/kg). Afterward, the blood glucose levels were observed within 24 d using a glucometer.

Results: The nanoparticles F1 displayed particle size of 157.8±18.0 nm with a polydispersity index of 0.488 and zeta potential of+51.4±4.56 mV. Spray drying process of the suspension of nanoparticles F1 produced a fine yellow powder. In contrast, nanoparticles F2 assembled with a different solvent system gave rise to a smaller particles size (90±20.8 nm) but could not be dried. Hence, the nanoparticles F1 were further studied for the antidiabetic study. The results showed that the nanoparticles; F1 rendered a better antidiabetic activity compared to the extracts. Percentage of decrease in blood glucose levels of the extract and the nanoparticles were 39.62 and 47.52%, respectively.

Conclusion: The type of solvent system of the extract could interfere with the resulting particle size of the nanoparticles. The nanoparticles could be promising carriers for the extract to improve the antidiabetic activity.

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References

Purnamasari D. The emergence of non-communicable disease in Indonesia. Acta Med Indones Indones J Intern Med 2018;50:273-4.

Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.

Kooti W, Farokhipour M, Asadzadeh Z, Ashtary Larky D, Asadi Samani M. The role of medicinal plants in the treatment of diabetes: a systematic review. Electron Physician 2016;8:1832-42.

Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 2014;4:1-10.

Zhang DW, Fu M, Gao SH, Liu JL. Curcumin and diabetes: a systematic review. Evid Based Complementary Alternat Med 2013:1-16. Doi:10.1155/2013/636053

Silva SBD, Amorim M, Fonte P, Madureira R, Ferreira D, Pintado M, et al. Natural extracts into chitosan nanocarriers for rosmarinic acid drug delivery. Pharm Biol 2015;53:642-52.

Liu Y, Feng N. Nanocarriers for the delivery of active ingredients and fractions extracted from natural products used in traditional Chinese medicine (TCM). Adv Colloid Interfac 2015;221:60-7.

Kwon TK, Kim JC. In vitro skin permeation and anti-atopic efficacy of lipid nanocarriers containing water-soluble extracts of Houttuynia cordata. Drug Dev Ind Pharm 2014;40:1350-7.

Kharia AA, Singhai AK, Verma R. Formulation and evaluation of polymeric nanoparticles of an antiviral drug for gastroretention. Int J Pharm Sci Nanotechnol 2012;4:1557-62.

Munawar AM, Jaweria TMS, Kishor M, Ellen KW. An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics 2017;9:1-26.

Barkat MA, Mujeeb M. Comparative study of Catharanthus roseus extract and extract loaded chitosan nanoparticles in alloxan-induced diabetic rats. Int J Biomed Res 2013;4:670-8.

Bora J, Syiem D, Bhan S. Methanolic flower extract of Phlogacanthus thyrsiflorus nees. Attenuates diabetic nephropathy in alloxan-induced diabetic mice. Asian J Pharm Clin Res 2018;11:113-6.

Pal S, Gautam S, Mishra A, Maurya R, Srivastava AK. Antihyperglycemic and antidyslipidemic potential of Ipomoea batatas leaves invalidated diabetic animal models. Int J Pharm Pharm Sci 2015;7:176-86.

Nagalakshmi K, Sujatha S. Nanoencapsulation augments release efficacy and glucose tolerance of 14-deoxy, 11, 12-didehydro, andrographolide loaded polycaprolactone nanoparticles in streptozotocin-nicotinamide induced type 2 diabetes. Int J Appl Pharm 2017;9:51-3.

Komalavalli T, Packia LM, Muthukumarasamy S, Mohan VR. Antidiabetic and antihyperlipidaemic activity of Sonerila tinnevelliensis fischer whole plant in alloxan-induced diabetic rats. Int J Curr Pharm Res 2015;7:53-7.

Kaliamurthi S, Selvaraj G. Insight on solid lipid nanoparticles: characterization and application in diabetes mellitus. J Crit Rev 2016;3:11-6.