ANTIOXIDANT ACTIVITY TEST OF FRACTIONS FROM STAR FRUIT LEAVES (AVERRHOA CARAMBOLA L.) FROM THREE REGIONS IN WEST JAVA

Authors

  • ANNISA DHANIRA Faculty of Pharmacy, Universitas Indonesia, Depok, 16424 West Java, Indonesia
  • BERNA ELYA Faculty of Pharmacy, Universitas Indonesia, Depok, 16424 West Java, Indonesia
  • KATRIN BASAH Faculty of Pharmacy, Universitas Indonesia, Depok, 16424 West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s1.FF019

Keywords:

Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Fractionation, Ferric reducing ability of plasma, Star fruit

Abstract

Objectives: Previous in vitro research showed that ethyl acetate fractions of star fruit leaves exhibit strong antioxidant activities toward 2,2-diphenyl-
1-picrylhydrazyl (DPPH) radical (IC50 = 90 μg/ml). This study’s aim was to evaluate antioxidant activity of fractions from leaf extracts from star fruit
sourced from three different regions in Indonesia (Depok, Sukabumi, and Subang) and, for the most active fraction (that with the highest antioxidant
activity), determine whether there was any correlation with phenolic and flavonoid content.
Methods: Liquid–liquid partitioning, fractions were evaluated for in vitro antioxidant activity using DPPH radical scavenging and ferric reducing
ability of plasma (FRAP) assay.
Results: The ethyl acetate fraction from the Subang region exhibited the strongest radical scavenging activity with both the DPPH assay (IC50 = 96 μg/ml)
and the FRAP assay (FeEAC value = 1405 μmol/g).
Conclusion: Ethyl acetate fractions from star fruit leaves could be used as natural antioxidants. No correlation was found between antioxidant activity
and phenolic or flavonoid content.

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Published

23-03-2020

How to Cite

DHANIRA, A., ELYA, B., & BASAH, K. (2020). ANTIOXIDANT ACTIVITY TEST OF FRACTIONS FROM STAR FRUIT LEAVES (AVERRHOA CARAMBOLA L.) FROM THREE REGIONS IN WEST JAVA. International Journal of Applied Pharmaceutics, 12(1), 97–100. https://doi.org/10.22159/ijap.2020.v12s1.FF019

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