DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS

Authors

  • YUNITRIANTI Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • BERNA ELYA Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • ARIKADIA NOVIANI Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s1.FF021

Keywords:

Antioxidants, Ayapana triplinervis (Vahl), 2,2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Determination of phenol, Determination of flavonoid

Abstract

Objective: Prasman (Ayapana triplinervis [Vahl]) is a plant that can eliminate free radicals through its antioxidant effects. However, little research has
been conducted to explore the antioxidant activity of the plant.
Methods: A. triplinervis (Vahl) leaves, which were determined by the Indonesian Institute of Sciences (LIPI) Bogor, were used in this study. Meanwhile,
extraction was performed using n-hexane, ethyl acetate, and methanol as the solvents. Based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay using
a UV–visible spectrophotometer and based on ferric reducing antioxidant power (FRAP) assay using a microplate reader.
Results: Based on the DPPH assay using a UV–visible spectrophotometer, n-hexane, ethyl acetate, and methanol extracts with a final concentration
of 25 μg/mL inhibited DPPH radical production by 38.91, 51.03, and 54.06%, respectively. Using the percent inhibition, the IC50 for the ethyl acetate
and methanol extracts were 28.71 and 23.472 μg/mL, respectively. Based on FRAP assay using a microplate reader, the n-hexane, ethyl acetate, and
methanol extracts had ferrous equivalent antioxidant capacity values of 460, 828.99, and 940.22 μmol/g, respectively. The methanol extract had the
greatest antioxidant activity. The ethyl acetate and methanol extracts at the initial concentrations contained total phenol levels of 12.06 and 42.11 mg
GAE/g extract, respectively, as well as total flavonoid levels of 3.24 and 3.41 mg QE/g extract, respectively.
Conclusion: Based on the determination of phenol and flavonoid levels, the methanol extract had the greatest antioxidant effects.

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Published

23-03-2020

How to Cite

YUNITRIANTI, ELYA, B. ., & NOVIANI, A. . (2020). DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS. International Journal of Applied Pharmaceutics, 12(1), 107–111. https://doi.org/10.22159/ijap.2020.v12s1.FF021

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