STABILITY STUDY OF ETHYLCELLULOSE COATED-TOCOTRIENOL MICROCAPSULES PREPARED BY SOLVENT EVAPORATION AND SPRAY DRYING TECHNIQUES

Authors

  • SILVIA SURINI Laboratory of Pharmaceutics and Pharmaceutical Technology Development, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • NADIA HUSNUL KHOTIMA Laboratory of Pharmaceutics and Pharmaceutical Technology Development, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s1.FF045

Keywords:

Microencapsulation, Solvent evaporation, Spray drying, Stability test, Tocotrienol

Abstract

Objective: Tocotrienol is a natural Vitamin E compound with greater antioxidant activity than tocopherol. However, tocotrienol is considered unstable,
which limits its handling and use in various product formulations. In this study, to enhance the stability of tocotrienol, tocotrienol oil was converted
into a powder through a microencapsulation method using ethylcellulose (EC) as the coating material.
Methods: Tocotrienol microcapsules were formulated with EC in ratios of 1:2 and 1:3 by solvent evaporation (SE) and spray drying techniques.
The obtained microcapsules were then characterized in terms of shape and morphology, particle size, entrapment efficiency, percentage yield,
flow properties, water content, swelling, and drug release. In addition, stability studies at both room temperature and elevated temperatures were
performed.
Results: Our results demonstrated that the tocotrienol microcapsules were of a white-yellowish powder of irregular shape, with particle sizes between
1 μm and 60 μm and entrapment efficiency of 21.60% and 99.75%. After 12 weeks of storage at room temperature, the remaining level of tocotrienol
in the microcapsules was 96.46–97.74%. In the accelerated stability study at elevated temperatures, the resulting k25 values ranged from 1.02×10-5 to
1.32×10-5/h. Thus, the predicted shelf-life (t90) of the microencapsulated tocotrienol was determined to be between 11.01 and 14.27 months.
Conclusion: The microencapsulation of tocotrienol with EC using SE and spray drying techniques produced a solid form of tocotrienol that was
considerably more stable than the natural form of tocotrienol.

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References

1. Aggarwal BB, Sundaram C, Prasad S, Kannappan R. Tocotrienols, the
Vitamin E of the 21st century: Its potential against cancer and other
chronic diseases. Biochem Pharmacol 2010;80:1613-31.
2. Packer L, Weber SU, Rimbach G. Molecular aspects of alpha-tocotrienol
antioxidant action and cell signalling. J Nutr 2001;131:369S-73S.
3. Rossi M, Alamprese C, Ratti S. Tocopherols and tocotrienols as free
radical-scavengers in refined vegetable oils and their stability during
deep-fat frying. Food Chem 2006;102:812-7.
4. Bakan JA, Anderson J. Microencapsulation. In: Lachman L,
Liebermann H, Kanig J, editors. The Theory and Practice of Industrial
Pharmacy. Vol. 3. Philadelphia, PA: Lea and Febiger; 1970. p. 384-407.
5. Pachuau L, Mazumder B. A study on the effects of different surfactants
on ethylcellulose microspheres. Int J Pharmtech Res 2009;1:966-71.
6. Pande AV, Vaidya PD, Arora A, Dhoka MV. In vitro and in vivo evaluation
of ethyl cellulose based floating microspheres of cefpodoxime proxetil.
Int J Pharm Biomed Res 2010;1:122-8.
7. Emami J, Varshosaz J. Ahmadi F. Preparation and evaluation of a liquid
sustained-release drug delivery system for theophylline using spraydrying
technique. Res Pharm Sci 2007;2:1-11.
8. Eduard A. Influence of key parameters on the morphology of
ethylcellulose microcapsules prepared via room-temperature spray
drying. Cellulose 2010;17:1-10.
9. Panda S, Pattnaik S, Maharana L, Botta GB, Mohapatra P. Formulation
and evaluation of zidovudine loaded olibanum resin microcapsules:
Exploring the use of natural resins as a biodegradable polymeric
material for controlled release. Asian J Pharm Clin Res 2013;6:191-6.
10. Surini S, Anggriani V, Anwar E. Study of mucoadhesive microspheres
based on pregelatinized cassava starch succinate as a new carrier for
drug delivery. J Med Sci 2009;9:249-56.
11. Jyothi NV, Prasanna PM, Sakarkar SN, Prabha KS, Ramaiah PS,
Srawan GY. Microencapsulation techniques, factors influencing
encapsulation efficiency. J Microencapsul 2010;27:187-97.
12. McNamee BF, O’Riordan ED, O’Sullivan M. Emulsification and
microencapsulation properties of gum Arabic. J Agric Food Chem
1998;46:4551-5.
13. Xie Y, Zhou H, Liang X, He B, Han X. Study on the morphology,
particle size and thermal microencapsulated by starch octenyl succinate.
Agric Sci China 2010;9:1058-64.
14. Fan-Long J, Soo-Jin P. Preparation and characterization of biodegradable
antibiotic-containing poly (?-caprolactone) microcapsules. J Ind Eng
Chem 2007;13:608-13.
15. Murtaza G, Ahmad M, Khan SA. Release behavior of different
physicochemical properties drug models from the ethylcellulose
microcapsules. J Pharm Bioallied Sci 2010;2:153.
16. Florence AT, Attwood D. Physicochemical Principles of Pharmacy.
USA: Pharmaceutical Press; 2006. p. 4.
17. Simonne AH, Simonne EH, Eitenmiller RR. Retention of Vitamin E and
added retinyl palmitate in selected vegetable oils during deep-fat frying
and in fried breaded products. J Agric Food Chem 1998;46:5273-7.

Published

23-03-2020

How to Cite

SURINI, S., & KHOTIMA, N. H. (2020). STABILITY STUDY OF ETHYLCELLULOSE COATED-TOCOTRIENOL MICROCAPSULES PREPARED BY SOLVENT EVAPORATION AND SPRAY DRYING TECHNIQUES. International Journal of Applied Pharmaceutics, 12(1), 197–201. https://doi.org/10.22159/ijap.2020.v12s1.FF045

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