The THE ANTICHOLESTEROL ACTIVITY OF BETULINIC ACID AND STIGMASTEROL ISOLATED FROM THE LEAVES OF SUNGKAI (PARONEMA CANESCENS JACK)

MUHARNI MUHARNI; FERLINAHAYATI FERLINAHAYATI; HENI YOHANDINI; FAHMA RIYANTI; NADYA ANASTASIA PRESCILLA PAKPAHAN

doi:10.22159/ijap.2021v13i2.40372

Authors

MUHARNI MUHARNI Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatra, 30662 Indonesia
FERLINAHAYATI FERLINAHAYATI Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatra, 30662 Indonesia
HENI YOHANDINI Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatra, 30662 Indonesia
FAHMA RIYANTI Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatra, 30662 Indonesia
NADYA ANASTASIA PRESCILLA PAKPAHAN Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatra, 30662 Indonesia

DOI:

https://doi.org/10.22159/ijap.2021v13i2.40372

Keywords:

Paronema canescens, Betulinic acid, Anticholesterol

Abstract

Objective: The purpose of this study was to evaluate the anticholesterol activity of Paronema canescens leaves extract.

Methods: These compounds were isolated via chromatography using silica gel as the stationary phase. Their structures were revealed using IR and 1D NMR data and by comparing their NMR data with the literature's data. The anticholesterol activity of compounds was tested using photometry via the Liebermann–Burchard reaction.

Results: Betulinic acid (1) and stigmasterol (2) were isolated from the leaves extracts of sungkai (P. canescens). The betulinic acid (1) that was isolated showed anticholesterol activity with an IC₅₀ value of 60.53 µg/ml, stigmasterol (2) 222.32 µg/ml, while the standard anticholesterol compound simvastatin had an IC₅₀ of 24.68 µg/ml.

Conclusion: The stigmasterol (2) has previously been reported from P. canescens while that of betulinic acid (1) is reported here for the first time and identifies its anticholesterol activity.

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