TURMERIC EXTRACT POTENTIAL INHIBIT INFLAMMATORY MARKER IN LPS-STIMULATED MARCOPHAGE CELLS

WAHYU WIDOWATI; DIANA KRISANTI JASAPUTRA; KAMILA YASHFA GUNAWAN; HANNA SARI WIDYA KUSUMA; SEILA ARUMWARDANA; CINTANI DEWI WAHYUNI; I. NYOMAN EHRICH LISTER; CHRISMIS NOVALINDA GINTING; ERMI GIRSANG; RIZAL RIZAL

doi:10.22159/ijap.2021.v13s3.01

Authors

WAHYU WIDOWATI Faculty of Medicine, Maranatha Christian University, Bandung 40163, West Java, Indonesia
DIANA KRISANTI JASAPUTRA Faculty of Medicine, Maranatha Christian University, Bandung 40163, West Java, Indonesia
KAMILA YASHFA GUNAWAN Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia
HANNA SARI WIDYA KUSUMA Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia
SEILA ARUMWARDANA Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia
CINTANI DEWI WAHYUNI Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia
I. NYOMAN EHRICH LISTER Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia
CHRISMIS NOVALINDA GINTING Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia
ERMI GIRSANG Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia
RIZAL RIZAL Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia, Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16426, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2021.v13s3.01

Keywords:

Inflammation, Turmeric, TNFalpha, IL-6, IL-1Beta

Abstract

Objective: Inflammation can be induced by microbiological, chemical, physical factors and plays roles in inflammatory diseases. Turmeric (Curcuma longa L.) has been widely used to provide a diverse array of biological activities, including anti-inflammatory, antimicrobial, also antioxidant. The Turmeric extract (TE) anti-inflammatory potential was conducted using a Lipopolysaccharide (LPS)-induced RAW264.7 macrophage cell line by inhibiting inflammatory mediators especially IL-6, PGE-2, IL-1β, COX-2, TNF-α, iNOS, also NO level.

Methods: The TE safe concentration in LPS-induced macrophage cell line was measured using MTS assay for further assay. The inflammatory markers (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO) were measured using ELISA assay and NO by the nitrate/nitrite colorimetric assay in LPS-induced RAW264.7 cell line. LPS induced inflammatory marker by increasing inflammatory marker (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO).

Results: TE with 100 to 25 µg/ml, caused a significant reduction of cells viability, reaching only 30.27 % live cells. TE with lower concentrations (7.5; 5; 2.5 µg/ml) had no cytotoxic effect on macrophage cells (viability 117.31-131.08 %). LPS induction caused an increase in inflammatory cytokines IL-1β, PGE-2, IL-6, COX-2, TNF-α as well as iNOS and NO. Turmeric extract caused the reduction of the inflammatory cytokines in a dose-dependent manner.

Conclusion: The research resulted that TE has anti-inflammatory activity by decreasing IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, and NO level on LPS-induced RAW264.7 cells.

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References

Jin R, Yang G, Li G. Inflammatory mechanisms in ischemic stroke: role of inflammatory cells. J Leukocyte Biol 2010;87:779–89.

Petersen AMW, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol 2005;98:1154–62.

Abrahamsen B, Zhou J, Asante CO, Cendan CM, Marsh S, Martinez Barbera JP, et al. The cell and molecular basis of mechanical, cold, and inflammatory pain. Sci 2008;321:702–5.

Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 2017;9:7204-18.

Sharma JN, Al-Omran A, Parvathy SS. Review role of nitric oxide in inflammatory disease. Inflammopharmacology 2007;15:252-9.

Wu YS, Chen SN. Apoptotic cell: linkage of inflammation and wound healing. Front Pharmacol 2014;5:1-6.

Lam D, Harris D, Qin Z. Inflammatory mediator profiling reveals immune properties of chemotactic gradients and macrophage mediator production inhibition during thioglycollate elicited peritoneal inflammation. Mediators Inflamm 2013:1-9.

Manfredi AA, Baldini M, Camera M, Baldissera E, Brambilla M, Peretti G, et al. Anti-TNFα agents curb platelet activation in patients with rheumatoid arthritis. Annals Rheumatic Diseases 2016;75:1511-20.

Page MJ, Bester J, Pretorius E. The inflammatory effects of TNF-α and complement component 3 on coagulation. Sci Reports 2018;8:1-9.

Dinarello CA. A clinical perspective of IL-1β as the gatekeeper of inflammation. Eur J Immunol 2011;41:1203-17.

Rose John S, Scheller J, Elson G, Jones SA. Interleukin-6 biology is coordinated by membrane-bound and soluble receptors: role in inflammation and cancer. J Leukoc Biol 2006;80:227-36.

Aggarwal BB, Yuan W, Li S, Gupta SC. Curcumin-free turmeric exhibits anti-inflammatory and anticancer activities: identification of novel components of turmeric. Mol Nutr Food Res 2013;57:1529-42.

Kocaadam B, Şanlier N. Curcumin, an active component of turmeric (Curcuma longa), and its effects on health. Crit Rev Food Sci Nutr 2017;57:2889-95.

Widowati W, Sardjono CT, Wijaya W, Laksmiwati DR, Darsono L. Free radicals scavenging of spices and curcumin. Proceedings of the Second International Syposium on Temulawak and the 40th Meeting of National Working Group on Indonesian Medicinal Plant; 2011.

Widowati W, Darsono L, Suherman J, Fauziah N, Maesaroh M, Erawijantari PP. Anti-inflammatory effect of mangosteen (Garcinia mangostana L.) peel extract and its compounds in LPS-induced RAW264.7 cells. Nat Prod Sci 2016;22:147-53.

Laksmitawati DR, Widyastuti A, Karami N, Afifah E, Rihibiha DD, Nufus H, et al. Anti-inflammatory effects of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-stimulated macrophage cell line. Bangladesh J Pharmacol 2017;12:35-40.

Arina N, Djamhuri DS, Nurhayati B, Rihibiha DD, Afifah E, Widowati W. Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells. Asian Pacific J Tropic Biomed 2017;7:1005–9.

Sandhiutami NMD, Moordiani M, Laksmiwati DR, Fauziah N, Maesaroh M, Widowati W. In vitro assesment of anti-inflammatory activities of coumarin and Indonesian cassia extract in RAW 264.7 murine macrophage cell line. Iran J Basic Med Sci 2017;20:99-106.

Widowati W, Prahastuti S, Ekayanti NLW, Munshy UZ, Kusuma HSW, Wibowo SHB, et al. Anti-Inflammation assay of black soybean extract and its compounds on lipopolysaccharide-induced RAW 264.7 cell. J Physics: Conference Series 2019;1374:1-11.

Pluemsamran T, Onkoksoong T, Panich U. Caffeic acid and ferulic acid inhibit UVA-induced matrix metalloproteinase-1 through regulation of antioxidant defense system in keratinocyte HaCaT cells. Photochem Photobiol 2012;88:961-8.

Singh N, Sundar S. Inflammatory chemokines and their receptors in human visceral leishmaniasis: gene expression profile in peripheral blood, splenic cellular sources and their impact on trafficking of inflammatory cells. Mol Immunol 2017;85:111–9.

Mahajna S, Azab M, Zaid H, Farich BA, Al Battah FF, Mashner S, et al. In vitro evaluations of cytotoxicity and antiinflammatory effects of Peganum harmala seed extracts in THP-1-derived macrophages. Eur J Med Plants 2015;5:165-75.

Bruderer M, Alini M, Stoddart M. Role of HOXA9 and VEZF1 in endothelial biology. J Vasc Res 2013;50:265–78.

Zhao C, Yang J, Wang Y, Liang D, Yang X, Li X, et al. Synthesis of mono-carbonyl analogues of curcumin and their effects on inhibition of cytokine release in LPS-stimulated RAW 264.7 macrophages. Bioorg Med Chem 2010;18:2388–93.

Aggarwal BB, Gupta SC, Sung B. Curcumin: an orally bioavailable blocker of TNF and other pro-inflammatory biomarkers. Br J Pharmacol 2013;169:1672-92.

Zhang QY, Mo ZN, Liu XD. Reducing effect of curcumin on expressions of TNF-alpha, IL-6 and IL-8 in rats with chronic nonbacterial prostatitis. Zhonghua Nan Ke Xue 2010;16:84–8.

Woo HM, Kang JH, Kawada T, Yoo H, Sung MK, Yu R. Active spice-derived components can inhibit inflammatory responses of adipose tissue in obesity by supressing inflammatory actions of macrophages and release of monocyte chemoattractant protein-1 from adipocytes. Life Sci 2007;80:926–31.

Jin CY, Lee JD, Park C, Choi YH, Kim GY. Curcumin attenuates the release of pro-inﬂammatory cytokines in lipopolysaccharide-stimulated BV2 microglia. Acta Pharmacol Sin 2007;28:1645–51.

Lee HS, Jung KK, Cho JY, Rhee MH, Hong S, Kwon M, et al. Neuroprotective effect of curcumin is mainly mediated by blockade of microglial cell activation. Pharmazie 2007;62:937–42.

Wang SL, Li Y, Wen Y, Chen YF, Na LX, Li ST, et al. Curcumin, a potential inhibitor of up-regulation of TNF-alpha and IL-6 induced by palmitate in 3T3-L1 adipocytes through NF-kappa B and JNK pathway. Biomed Environ Sci 2009;22:32–9.

Zhang L, Wu C, Zhao S, Yuan D, Lian G, Wang X, et al. Demethoxycurcumin, a natural derivative of curcumin attenuates LPS-induced pro-inﬂammatory responses through down-regulation of intracellular ROS-related MAPK/NF-kappa B signaling pathways in N9 microglia induced by lipopolysaccharide. Int Immunopharmacol 2010;10:331–8.

Liang G, Li X, Chen L, Yang S, Wu X, Studer E, et al. Synthesis and anti-inﬂammatory activities of mono-carbonyl analogues of curcumin. Bioorg Med Chem Lett 2008;18:1525–9.

Mosser DM, Edwards JP. Exploring the full spectrum of macrophage activation. Nat Rev Immunol 2008;8:958-69.

Ledoux AC, Perkins ND. NF-κB and the cell cycle. Biochem Soc Trans 2014;42:76-81.

Lee CW, Kim SC, Kwak TW, Lee JR, Jo MJ, Ahn YT, et al. Anti-inflammatory effects of bangpungtongsung-San a traditional herbal prescription. Evidence-Based Complement Altern Med 2012;892943:1-12.

Alam Md B, Ju MK, Kwon YG, Lee SH. Protopine attenuates inflammation stimulated by carrageenan and LPS via the MAPK/NF-kB pathway. Food Chem Toxicol 2019;131:110583.

Kunnumakkara AB, Bordoloi D, Padmavathi G, Monisha J, Roy NK, Prasad S, et al. Curcumin, the golden nutraceutical: multitargeting for multiple chronic diseases. Br J Pharmacol 2017;174:1325-48.