CHARACTERIZATION OF NANOPARTICLES CONDITIONED MEDIUM ADIPOSE TISSUE MESENCHYMAL STEM CELL (CM-ATMSC)

DENI RAHMAT; WAHYU WIDOWATI; AHMAD FARIED; ITA MARGARETHA NAINGGOLAN; DIDIK PRIYANDOKO; ANARISA BUDIATI; TEDDY MARCUS ZAKARIA; ERVI AFIFAH

doi:10.22159/ijap.2022.v14s3.22

Authors

DENI RAHMAT Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
WAHYU WIDOWATI Maranatha Christian University, Jl. Surya Sumantri No 65, Bandung 40164, West Java, Indonesia
AHMAD FARIED Faculty of Medicine, Padjadjaran University, JL. Raya Bandung Sumedang, Jatinangor, West Java, Indonesia
ITA MARGARETHA NAINGGOLAN Atma Jaya Catholic University, Jl. Jenderal Sudirman No.51, South Jakarta 12930, Indonesia
DIDIK PRIYANDOKO Faculty of Mathematic and Natural Science Education, Indonesia University of Education, Jl. Dr. Setiabudi No. 229, Bandung 40154, West Java, Indonesia
ANARISA BUDIATI Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
TEDDY MARCUS ZAKARIA Maranatha Christian University, Jl. Surya Sumantri No 65, Bandung 40164, West Java, Indonesia
ERVI AFIFAH PT Aretha Medika Utama, Jl. Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s3.22

Keywords:

Nanoparticles, Drug delivery, Conditioned medium, Stem cells

Abstract

Objective: The aims of this study were to formulate and characterize the nanoparticles containing conditioned medium adipose tissue

mesenchymal stem cell (CM-ATMSC).

Methods: Adipose Tissue Mesenchymal Stem Cell (AT-MSC) was cultured with supplemented Modified Eagle Medium (MEM) Alpha in an incubator with 37 °C and 5% CO2. The conditioned medium was collected when the cells were confluent. Nanoparticles were characterized in the term of morphology using transmission electron microscopy (TEM), particle size, zeta potential, loading capacity, entrapment efficiency, and drug release.

Results: CM-ATMSC nanoparticles had the smallest particle size, namely chitosan-inulin nanoparticles with particle size of 128 nm, and the largest particle was modified chitosan (thiomer)-fucoidan nanoparticles with particle size of 254.3 nm. Based on zeta potential results, it is known that the resulting nanoparticle suspension was stable.

Conclusion: The resulting nanoparticle suspension was stable and the smallest CM-ATMSC nanoparticle demonstrated the particle size of 128 nm. The results showed that the nanoparticles of CM-ATMSC have been successfully prepared.

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