METHOD VALIDATION OF SIMVASTATIN IN PCL-PEG-PCL TRIBLOCK COPOLYMER MICELLES USING UV-VIS SPECTROPHOTOMETRIC FOR SOLUBILITY ENHANCEMENT ASSAY

DEWI PATMAYUNI; T. N. SAIFULLAH SULAIMAN; ABDUL KARIM ZULKARNAIN; SHAUM SHIYAN

doi:10.22159/ijap.2022v14i1.42961

Authors

DEWI PATMAYUNI Bhakti Pertiwi, School of Pharmacy Science, Palembang 30128 Indonesia
T. N. SAIFULLAH SULAIMAN Departement of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281 Indonesia
ABDUL KARIM ZULKARNAIN Departement of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281 Indonesia
SHAUM SHIYAN Departement of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indralaya 30622 Indonesia

DOI:

https://doi.org/10.22159/ijap.2022v14i1.42961

Keywords:

Simvastatin, Triblock copolymer, PCL, PEG, Validation, Uv-Vis spectrophotometric

Abstract

Objective: This study aims to increase the solubility of simvastatin (SIM), a hydrophobic drug, by incorporating it into PCL-PEG-PCL triblock copolymer micelles and validating the assay method used, namely Uv-Vis spectrophotometric.

Methods: The shake flask method was used to determine the increase in solubility experienced by SIM after being incorporated into the micellar system. The values of maximum wavelength (λ_max), linearity, LOD, LOQ, accuracy, and precision were used as parameters measured to assess the validity of the assay method used.

Results: The results showed that PCL-PEG-PCL triblock copolymer micelles could increase SIM solubility by 9.7 times (89.49±5.75 µg/ml) compared to SIM without modification (9.19±0.24 µg/ml). The validation results show the λ_max value of 239 nm, a linear calibration curve with an R-value of 0.9994, LOD and LOQ of 0.33 µg/ml and 1.00 µg/ml, accurate measurement with recovery at concentrations of 80%, 100%, and 120% were 102.93±1.32%, 100.78±0.40%, and 104.58±0.79% and also had good precision with RSD<2%.

Conclusion: The PCL-PEG-PCL triblock copolymer micelles can increase SIM solubility and the Uv-Vis spectrophotometric method has been validated successfully for the quantitative analysis of SIM in PCL-PEG-PCL triblock copolymer micelles.

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