STABILITY AND ANTIBACTERIAL ACTIVITY TEST OF NANOSILVER BIOSYNTHETIC HYDROGEL
DOI:
https://doi.org/10.22159/ijap.2022v14i2.43584Keywords:
Antibacterial, Cycling test, Turmeric juice, Nanosilver, HydrogelAbstract
Objective: This study aims to formulate nanosliver (AgNPs) biosynthetic hydrogel for topical antibacterial treatment and its stability and antibacterial activity.
Methods: The mixture (Silver nitrate solution and Turmeric juice) was stirred at room temperature for 24 h; afterward, it was analyzed using UV-VIS spectrophotometry, particle size analysis, and TEM. The carbopol 940 was selected as a gelling agent with an AgNPs concentration of 5%, 10%, 20%, and 30%. Furthermore, the gel preparation was tested for stability using the cycling test method and antibacterial activity. The antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa.
Results: The nanosilver biosynthetic has a yellow-brown color with the maximum wavelength peak at 433 nm, and a particle size of 157.4-166.7 nm. TEM analysis showed that AgNPs have a round shape, while the antibacterial activity of hydrogel preparations was moderately inhibited. Furthermore, the hydrogel was evaluated for pH, viscosity, dispersibility, and antibacterial activity before-after the cycling test. Formula with 30% AgNPs is chosen formula with pH value of 5.87±0.65; viscosity of 4833.3±2.82 c. Ps; and dispersibility of 5.50±0.15 after cycling test.
Conclusion: The high concentration of AgNPs will increase the viscosity, pH, and dispersibility. Formula with 30% AgNPs have the highest antibacterial activity. Furthermore, all hydrogel preparations meet the requirements of Indonesian Standard Product (SNI) No. 06-2588-1992 for good gel stability before and after the cycling test.
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El Khoury, Elsy, Mohamad A, Zeina G, Kassaify, El Khoury E, Abiad M, Kassaify ZG, Patra D. Green synthesis of curcumin conjugated nanosilver for the applications in nucleic acid-sensing and anti-bacterial activity. Colloids Surf B Biointerfaces. 2015;127:274-80. doi: 10.1016/j.colsurfb.2015.01.050, PMID 25687098.
Ahmed S, Ahmad M, Swami BL, Ikram S. A review on plants extract mediated synthesis of Silver nanoparticles for antimicrobial applications: A green expertise. J Adv Res. 2016;7(1):17-28. doi: 10.1016/j.jare.2015.02.007.
Shankar SS, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, ag, and bimetallic Au core-ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Colloid Interface Sci. 2004;275(2):496-502. doi: 10.1016/j.jcis.2004.03.003, PMID 15178278.
Shameli K, Ahmad MB, Zamanian A, Sangpour P, Shabanzadeh P, Abdollahi Y, Zargar M. Green biosynthesis of silver nanoparticles using curcuma longa tuber powder. Int J Nanomedicine. 2012;7:5603-10. doi: 10.2147/IJN.S36786, PMID 23341739.
Kurian M, Varghese B, Athira TS, Krishna S. Novel and efficient synthesis of silver nanoparticles using Curcuma longa and Zingiber officinale rhizome extracts. Int J Nanosci Nanotechnol. 2016;12(3):175-81.
Alsammarraie FK, Wang W, Zhou P, Mustapha A, Lin M. Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities. Colloids Surf B Biointerfaces. 2018;171:398-405. doi: 10.1016/j.colsurfb.2018.07.059, PMID 30071481.
Williamson DA, Carter GP, Howden BP. Current and emerging topical antibacterials and antiseptics: agents, action, and resistance patterns. Clin Microbiol Rev. 2017;30(3):827-60. doi: 10.1128/CMR.00112-16, PMID 28592405.
Humaida R. Strategy to handle the resistance of antibiotics. J Majority. 2014;3(7):113-20.
Goskonda SR. Handbook of pharmaceutical excipients. 6th ed. London: Pharmaceutical Press and American Pharmacists Association; 2009.
Budama Kilinc Y, Cakir Koc R, Zorlu T, Ozdemir B, Karavelioglu Z, Egil AC, Kecel Gunduz S. Assessment of nano-toxicity and safety profiles of silver nanoparticles, Silver nanoparticles-fabrication, characterization and applications. London: Intechopen; 2018.
Seltenrich N. Nanosilver: weighing the risks and benefits. Environ Health Perspect. 2013;121(7):A220-5. doi: 10.1289/ehp.121-a220, PMID 23816826.
Fujihastuti T, Sifat Fisik SN, Ekstrak Etanol DIG. Herba pegagan (Centella asiatica L.) dengan variasi jenis gelling agent. J Farmasi Indones. 2015;12(1):11-20.
Melani D, Purwanti T, Soeratri W. Korelasi kadar propilenglikol dalam basis dan pelepasan dietilammonium diklofenak dari basis gel 78 carbopol ETD. [Bagian Farmasetika fakultas farmasi. Universitas Airlangga]. Surabaya; 2020.
Prathna TC, Chandrasekaran N, Raichur AM, Mukherjee A. Biomimetic synthesis of silver nanoparticles by Citrus limon (Lemon) aqueous extract and theoretical prediction of particle size. Colloids Surf B Biointerfaces. 2011;82(1):152-9. doi: 10.1016/j.colsurfb.2010.08.036, PMID 20833002.
Sutanti F, Silvia D, Putri MA, Fabiani VA. Pengaruh konsentrasi AgNO3 pada sintesis nanopartikel perak menggunakan bioreduktor ekstrak pucuk idat (Cratoxylum glaucum Korth.). Proceedings National Colloquium Research Community Service. 2018;2:1-4.
Martin AJ, Swarbrick, Cammarata A. Farmasi fisik. Jakarta: UI Press; 2012.
Muadifah A, Amini HW, Amini HW, Putri AE, Putri AE, Latifah N, Latifah N. Aktivitas antibakteri ekstrak rimpang kunyit (Curcuma domestica Val) terhadap Bakteri Staphylococcus aureus. JSH. 2019;3(1):45-54. doi: 10.51804/jsh.v3i1.313.45-54.
Fahimirad S, Ajalloueian F, Ghorbanpour M. Synthesis and therapeutic potential of silver nanomaterials derived from plant extracts. Ecotoxicol Environ Saf. 2019;168:260-78. doi: 10.1016/j.ecoenv.2018.10.017, PMID 30388544.
Haryani Y, Rimpang Jahe PEA. Merah (Zingiber officinale Linn. Var. Rubrum pada biosintesis sederhana nanopartikel perak. Chimica et Natura Acta. 2016;4(3):151.
Rahayu S, Kurniasih N, Amalia V. Ekstraksi dan identifikasi senyawa flavonoid dari limbah kulit bawang merah sebagai antioksidan alami. AK. 2015;2(1):1-8. doi: 10.15575/ak.v2i1.345.
Campbell CSJ, Contreras Rojas LR, Delgado Charro MB, Guy RH. Objective assessment of nanoparticle disposition in mammalian skin after topical exposure. J Control Release. 2012;162(1):201-7. doi: 10.1016/j.jconrel.2012.06.024, PMID 22732479.
Nidhin M, Indumathy R, Sreeram KJ, Nair BU. Synthesis of iron oxide nanoparticles of narrow size distribution on polysaccharide templates. Bull Mater Sci. 2008;31(1):93-6. doi: 10.1007/s12034-008-0016-2.
Dominguez AV, Ayerbe Algaba R, Miro Canturri A, Rodriguez Villodres A, Smani Y. Antibacterial activity of colloidal silver against gram-negative and gram-positive bacteria. Antibiotics. 2020;9(36):1-10.
Patel J, Patel B, Banwait H, Parmar K, Patel M. Formulation and evaluation of topical aceclofenac Gel using the different gelling agent. Int J Drug Dev Res. 2011;3(1):156-64.
Saraung V, Yamlean PV, Citraningtyas G. Pengaruh variasi babis karbopol dan hpmc pada formulasi gel ekstrak etanol daun tapak kuda (Ipomoea pes-caprae (L.) R. Br. d. Uji aktivitas antibakteri terhadap Staphylococcus aureus. Pharmacon. 2018;7(3):220-9.
Annisa R, Suryadinata A, Nashichuddin A, Mutiah R, Fauziyah B. Development of an antimicrobial gel formulation for topical delivery using silver nanoparticle. Indian J Novel Drug Deliv. 2019;11(1):13-9.
Wahyudi T, Sugiyana D, Helmy Q. Sintesis nanopartikel perak dan uji aktivitasnya terhadap bakteri E. coli dan S. aureus. Arena Tekstil. 2011;26(1):55-60. doi: 10.31266/at.v26i1.1442.
Diez Pascual AM. Antibacterial action of nanoparticle loaded nanocomposites based on graphene and its derivatives: A mini-review. Int J Mol Sci. 2020;21(10):1-22. doi: 10.3390/ijms21103563, PMID 32443558.
Mishra M, Chauhan P. Nanosilver and its medical implications. J Nanomed Res. 2015;2(5):1-10.
Sirajudin A, Rahmanisa S. Nanopartikel perak sebagai penatalaksanaan infeksi saluran kemih. Majority. 2016;5(4):1-5.
Adila R, Nurmiati AA. Uji antimikroba Curcuma spp terhadap pertumbuhan Candida albicans, Staphylococcus aureus, dan Escherichia coli. J Biologi Univ Andalas. 2013;2(1):1-7.
Mursyid AM. Evaluasi stabilitas fisik dan profil difusi sediaan gel (Minyak Zaitun). IJPF. 2017;4(1):205-11. doi: 10.33096/jffi.v4i1.229.
Wulandari W, Ermawati DE, Yugatama A. Optimization SNEDDS (self-nano emulsifying drug delivery system) of ZnO that dispersed into hydrogel matrix as UV-protective. IOP conf ser. Mater Sci Eng. 2019;578(1):012058. doi: 10.1088/1757-899X/578/1/012058.
Pertiwi D, Desnita R, Luliana S. Pengaruh pH terhadap stabilitas alpha arbutin dalam gel niosom. Majalah Farmaseutik. 2020;16(1):91-100. doi: 10.22146/farmaseutik.v16i1.49446.
VA, GB, Grace JRL, Vennila R. Antibacterial effectiveness of prosopis juliflora against aerobic and anaerobic bacteria. Int J Curr Pharm Sci 2020;12(6):110-3. doi: 10.22159/ ijcpr.2020v12i6.40305.
Chippa S, Suvarna V. Nanotechnology for detection of diseases caused by viruses-current overview. Int J Pharm Pharm Sci. 2021;13(4):1-7. doi: 10.22159/ijpps.2021v13i4.40359.
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