ACUTE TOXICITY OF Β-KITIN EXTRACTED FROM THE SHELL OF BLUE SWIMMING CRAB (PORTUNUS PELAGICUS LINN.)

RENNY AMELIA; NYI MEKAR SAPTARINI; JUTTI LEVITA; SRI ADI SUMIWI

doi:10.22159/ijap.2021.v13s4.43843

Authors

RENNY AMELIA Department of Pharmacology and Clinical Pharmacy, Universitas Padjadjaran, Sumedang 45363, (West Java) Indonesia, Department of Pharmacology, School of Pharmacy Muhammadiyah Cirebon, Cirebon-45153, (West Java) Indonesia
NYI MEKAR SAPTARINI Department of Pharmaceutical Analysis and Medicinal Chemistry, Universitas Padjadjaran, Sumedang 45363, (West Java) Indonesia
JUTTI LEVITA Department of Pharmacology and Clinical Pharmacy, Universitas Padjadjaran, Sumedang 45363, (West Java) Indonesia
SRI ADI SUMIWI Department of Pharmacology and Clinical Pharmacy, Universitas Padjadjaran, Sumedang 45363, (West Java) Indonesia

DOI:

https://doi.org/10.22159/ijap.2021.v13s4.43843

Keywords:

β-chitin, Crab, Acute toxicity, Histopathology

Abstract

Objective: This work aimed to study the acute toxicity of β-chitin extracted from crab shells in Bal b/c mice.

Method: The acute toxicity test was performed by following the OECD guidelines. Female mice were given single or divided doses of β-chitin (maximum 24 h) with doses of 500, 1000, 2000, 4000, and 6000 mg/kg of BW. Observations were made for 14 d, including behaviour, body weight, organ weight, and histopathology of vital organs (stomach, heart, liver, kidney, and lung).

Results: During 14 d, no deaths and no abnormalities in behaviour, bodyweight or organ weight were observed. Qualitative histopathological observations at the highest dose showed abnormalities of the liver and kidney compared to those of the control group. Nevertheless, the abnormalities did not affect the organ function.

Conclusion: This acute toxicity study reveals that β-chitin up to a dose of 6000 mg/kg of BW is not toxic, as proved by the normal behaviour, body weight, and vital organ weight of the animals. Further chronic toxicities study is needed to confirm its safety.

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