ACTIVITY NANOKIRINYUH (CHROMOLAENA ODORATA) LEAVES EXTRACT IN ALLOXAN INDUCED DIABETIC RATS

KENI IDACAHYATI; WINDA TRISNA WULANDARI; FIRMAN GUSTAMAN; INDRA LUSI NURDIANTI

doi:10.22159/ijap.2021.v13s4.43863

Authors

KENI IDACAHYATI Department of Clinical Pharmacy and Pharmacology, Bakti Tunas Husada Institute of Health Science, Tasikmalaya, Indonesia, Cilolohan Street No 36, Tasikmalaya 46115
WINDA TRISNA WULANDARI Department of Clinical Pharmacy and Pharmacology, Bakti Tunas Husada Institute of Health Science, Tasikmalaya, Indonesia, Cilolohan Street No 36, Tasikmalaya 46115
FIRMAN GUSTAMAN Department of Clinical Pharmacy and Pharmacology, Bakti Tunas Husada Institute of Health Science, Tasikmalaya, Indonesia, Cilolohan Street No 36, Tasikmalaya 46115
INDRA LUSI NURDIANTI Department of Clinical Pharmacy and Pharmacology, Bakti Tunas Husada Institute of Health Science, Tasikmalaya, Indonesia, Cilolohan Street No 36, Tasikmalaya 46115

DOI:

https://doi.org/10.22159/ijap.2021.v13s4.43863

Keywords:

Diabetes, Nanochitosan, Kirinyuh, Leaves

Abstract

Objective: Diabetes Mellitus is a type of degenerative disease that is increasing every year in countries around the world. Diabetes Mellitus is a major cause of blindness, kidney failure, heart attacks, and stroke. Nanokirinyuh leaves have potential as an antidiabetic because they contains chemical compounds that have antioxidant activity. The purpose of this study was to determine activity of nanokirinyuh leaves as an antidiabetic.

Methods: Wistar rats as many as 24 animals were divided into 6 groups, namely the normal control group, positive control (glibenclamide 0.5 mg/Kg BW), negative control (alloxan 600 mg/BW rat), and nanochitosan kirinyuh leaves at a dose of 225 mg/Kg BW rat, 450 mg/Kg BW rat and 675 mg/Kg BW treatment was carried out for 10 d. Percent decrease of level glucose was evaluated along with histopathological investigation in various experimental groups of rats. Data analysis using the One Way Anova test and continued LSD test.

Results: Level of Glucose at a dose of 675 mg/Kg BW rats showed the highest levels of the negative group and other dose groups. Pancreas histopathology test results showed that the group with a dose of 450 mg/Kg BW of rats had the lowest necrosis rate compared to the negative control group and other dose groups.

Conclusion: Nanokirinyuh leaves can reduce of level plasma glucose and necrosis in a histopathology test.

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