EFFECT OF OSTERIX AND OSTEOCALCIN ENHANCEMENT BY QUERCETIN (3,3′,4′,5,7-PENTAHYDROXYFLAVONE) ON OSTEOBLAST HFOB 1.19 CELL LINE

AGNIS PONDINEKA RIA ADITAMA; BURHAN MA’ARIF; FAISAL AKHMAL MUSLIKH

doi:10.22159/ijap.2022.v14s1.07

Authors

AGNIS PONDINEKA RIA ADITAMA Department of Pharmacy, Pharmacy Academy of Jember, Jember, 68125, Indonesia
BURHAN MA’ARIF Department of Pharmacy, Faculty of Medical and Health Science, Maulana Malik Ibrahim State Islamic University, Malang, 65151, Indonesia
FAISAL AKHMAL MUSLIKH Master Student of Pharmaceutical Science, Faculty of Pharmacy, Universitas Airlangga, Surabaya, 60115, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s1.07

Keywords:

Quercetin, Anti-osteoporosis, Osteoblast, hFOB 1.19 cell line, Osterix, Osteocalcin

Abstract

Objective: This study was aimed at investigating the effect of quercetin (3,3′,4′,5,7-pentahydroxyflavone) as a phytoestrogen in the treatment of estrogen deficiency-induced osteoporosis, through the measurement of osterix and osteocalcin expressions on osteoblast hFOB 1.19 cell line.

Methods: hFOB 1.19 cells were cultured in 24-well microplates, induced with 10 ng/ml TNF-α and incubated for 24 h. TNF-α induction was used to create an estrogen deficiency condition. Quercetin was then added at 10 µM concentration. The immunocytochemistry double staining method was performed with anti-rabbit osterix primary antibody and anti-mouse osteocalcin primary antibody. The cells were then incubated at 4 °C overnight. Finally, an anti-rabbit secondary antibody FITC and anti-mouse secondary antibody rhodamine were added before the cells were analyzed using a Confocal Instrument Laser Scanning Microscopy (CLSM) at 488 and 543 nm.

Results: Quercetin increased the expressions of both osterix and osteocalcin in the osteoblast hFOB 1.19 cell line compared to the negative controls (p<0.005), with expression values of 57852*±3878.71 AU and 24161.75*±1498.65 AU, respectively.

Conclusion: Quercetin shows an anti-osteoporosis effect by increasing the expressions of both osterix and osteocalcin in osteoblast hFOB 1.19 cell line.

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