• SREELAKSHMI S. Department of Rasashastra and Bhaishajya Kalpana (Pharmaceuticals), Amrita School of Ayurveda, Amrita Viswa Vidyapeetham, Amritapuri, Kollam, India
  • ARUN MOHANAN Department of Rasashastra and Bhaishajya Kalpana (Pharmaceuticals), Amrita School of Ayurveda, Amrita Viswa Vidyapeetham, Amritapuri, Kollam, India
  • VINEETH P. K Department of Rasashastra and Bhaishajya Kalpana (Pharmaceuticals), Amrita School of Ayurveda, Amrita Viswa Vidyapeetham, Amritapuri, Kollam, India




Decoction, Ayurveda, Amṛtottara Kaṣaya, Pravahi Kvatha, Kaṣaya Kalpan, Preservative, Sodium benzoate


Objective: Herbal decoctions are commonly used pharmaceutical dosage form in Ayurveda. The major drawback of decction is short shelf life. To increase the shelf-life preservatives are used. The effectiveness of decoctions added with preservative is not evaluated yet. Pravāhi kvātha is fermented decoctions prepared to increase the shelf life as well as the palatability of decoctions. In the present study, an attempt has been made compare the efficacy of freshly prepared herbal decoction, decoction added with preservative and fermented decoction.

Methods: To compare the efficacy, the three samples were subjected to analytical study through organoleptic, physicochemical and other advanced chromatographic and instrumental analysis. For scientifically validating the effectiveness the samples were analysed for Physico chemical parameters, HPTLC, Gallic acid estimation, HR LC-MS and DPPH assay.

Results: After the physico chemical analysis it was found that the parameters of three samples was within permissible limit. The anti-oxidant potential of three samples were proved by the DPPH free radical scavenging activity. The I50 value of three samples shows only less variation and all the sample possess good anti-oxidant potential. Phyto chemical evaluation of the three samples were compared wit HPTLC, Gallic acid estimation and HR LCMS analysis.

Conclusion: The study shows that the modification of Kaṣāya to Pravāhi Kvātha will not affect its therapeutic efficacy rather it adds more therapeutic value due to the presence of newly synthesized compounds. Addition of Sodium benzoate preservative doesn’t alter the therapeutic efficacy of Amṛtottara Kaṣāya and it won’t produce any toxic chemicals by the interaction with drugs.


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Deepthi CP, Basavaraj G, Sreekanth G, Rohit KS, Anu PK. Modifications of Pancha Vidha kashaya Kalpana. Unique J Ayurvedic Herb Med. 2015;3(5):60-3.

Pandit Parusurama Shastri, Sarangadhara Samhita with commentaries Dipika of Adhamalla, Gudartha Dipika of Kairama. New Delhi, Chaukhambha Publication. reprint ed;, Madhyama khanda, chapter 2; 2013. p. 102-9.

Namdev SR, Rakesh SR. Critical study of pharmaco-clinical basis of panchvidha kashaya Kalpana selection. International Ayurvedic Medical. 2016;4(8):2488-92.

Randive DS, Sayyad SF, Bhinge SD, Bhutkar MA. Preparation of Arjunariṣṭa using microbes isolated from Woodfordia fruticosa Flowers (Dhayati). Anc Sci Life. 2016;36(1):42-7. doi: 10.4103/0257-7941.195405, PMID 28182028.

Baidyanath Ayurved Sara Samgraha Baidyanath Priv Limited,edition; 2005. p. 718-25.

The Ayurvedic Formulary of India. 2nd ed. New Delhi: National Institute of Science Communication and Information Resources; 2003. p. 53.

Trikamji A Yadavji, Saṃhita C. With ayurveda dipika commentary, Varanasi. Chaukhamba Prakaśan Ed; 2013.

Acharya S. Sharngadhara S with the commentaries of Adhamall’s Dipika and Kashirama’s Gudhartha Dipika, Chaukhambha Orientalia; Varanasi edition; 2000. p. 522-31.

Stanbury PF, Whitker A, Hall SJ. Principles of fermentation technology. 2nd ed. New Delhi: Aditya books; 1997. p. 192-209.

Prashant S Bhokardankar, Balasubramani, Manjiri Prashant Bhokardankar. Estimation of gallic acid in different commercial samples of amruttotharam kasayam by using HPLC. PBJ 2018;6(3):41-5. doi: 10.20510/ukjpb/6/i3/175590.

Sulaiman CT, Anju K, Anandan EM, Balachandran I. Synergistic interactions of phytochemicals in polyherbal formulation enhance the chemical transformations of active constituents. J Appl Spectrosc. 2021;88(1):181-6. doi: 10.1007/s10812-021-01156-w.

Zhao HY, Lan Q, He S, Su BJ, Wang YQ, Liao HB. Chebulic acid derivatives from Balakata baccata and their antineuroinflammatory and antioxidant activities. Bioorg Chem. 2021 Nov;116:105332. doi: 10.1016/j.bioorg.2021.105332. PMID 34509045.

Musial C, Kuban Jankowska A, Gorska Ponikowska M. Beneficial properties of green tea catechins. Int J Mol Sci. 2020 Mar 4;21(5):1744. doi: 10.3390/ijms21051744, PMID 32143309, PMCID PMC7084675.

Bai J, Zhang Y, Tang C, Hou Y, Ai X, Chen X. Gallic acid: pharmacological activities and molecular mechanisms involved in inflammation-related diseases. Biomed Pharmacother. 2021 Jan;133:110985. doi: 10.1016/j.biopha.2020.110985. PMID 33212373.

Stavric B, Stoltz DR. Shikimic acid. Food Cosmet Toxicol. 1976 Apr;14(2):141-5. doi: 10.1016/s0015-6264(76)80259-3, PMID 773799.

Tanasiewicz M, Hildebrandt T, Obersztyn I. Xerostomia of various etiologies: a review of the literature. Adv Clin Exp Med. 2016 Jan-Feb;25(1):199-206. doi: 10.17219/acem/29375, PMID 26935515.

Cavagnero KJ, Badrani JH, Naji LH, Amadeo MB, Leng AS, Lacasa LD. Cyclic-di-GMP induces STING-dependent ILC2 to ILC1 shift during innate type 2 lung inflammation. Front Immunol. 2021 Feb 18;12:618807. doi: 10.3389/fimmu.2021.618807, PMID 33679760, PMCID PMC7935536.

Murugesan S, Kottekad S, Crasta I, Sreevathsan S, Usharani D, Perumal MK. Targeting COVID-19 (SARS-CoV-2) main protease through active phytocompounds of ayurvedic medicinal plants–Emblica officinalis (Amla), Phyllanthus niruri Linn. (Bhumi Amla) and Tinospora cordifolia (Giloy)–A molecular docking and simulation study. Comput Biol Med. 2021;136:104683. doi: 10.1016/j.compbiomed.2021.104683. PMID 34329860.

Aminoglutethimide. National center for biotechnology information. Lancet. 1981 May 30;1(8231):1194-5. PMID 6112535.

Yarrow JF, McCoy SC, Borst SE. Tissue selectivity and potential clinical applications of trenbolone (17beta-hydroxyestra-4,9,11-trien-3-one): A potent anabolic steroid with reduced androgenic and estrogenic activity. Steroids. 2010 Jun;75(6):377-89. doi: 10.1016/j.steroids.2010.01.019, PMID 20138077.

Pathak G, Singh S, Kumari P, Raza W, Hussain Y, Meena A. Cirsimaritin, a lung squamous carcinoma cells (NCIH-520) proliferation inhibitor. J Biomol Struct Dyn. 2021 Jun;39(9):3312-23. doi: 10.1080/07391102.2020.1763198. PMID 32362196.

Moreno D, Sobarzo CM, Lustig L, Rodriguez Pena MG, Guazzone VA. Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord. Asian J Androl. 2020 Jan-Feb;22(1):112-7. doi: 10.4103/aja.aja_30_19. PMID 31115365, PMCID PMC6958983.

Radomski L, Brody MJ. Nifedipine. J Iowa Med Soc. 1983 Jul;73(7):278-80. PMID 6350496.

Tuan Anh HL, Kim DC, Ko W, Ha TM, Nhiem NX, Yen PH. Anti-inflammatory coumarins from Paramignya trimera. Pharm Biol. 2017 Dec;55(1):1195-201. doi: 10.1080/13880209. 2017.1296001, PMID 28245363, PMCID PMC6130569.

Sivakumar S, Mohanasundaram S, Rangarajan N, Sampath V, Velayutham Dass Prakash MV. In silico prediction of interactions and molecular dynamics simulation analysis of Mpro of severe acute respiratory syndrome caused by novel coronavirus 2 with the FDA-approved nonprotein antiviral drugs. J Appl Pharm Sci. 2022;12(05):104-19. doi: 10.7324/JAPS.2022.120508.

Sawarkar G, Sawarkar P. Ayurvedic management of chronic pancreatitis in adolescent girl: A case report. JPRI. 2021;33(33B):58-67. doi: 10.9734/jpri/2021/ v33i33B31797.



How to Cite

S., S., MOHANAN, A., & P. K, V. (2022). A COMPARATIVE PHYSICO CHEMICAL ANALYSIS OF AMṚTOTTARA KVATHA AND IT’S PRAVAHI KVATHA. International Journal of Applied Pharmaceutics, 14, 116–121. https://doi.org/10.22159/ijap.2022.v14ti.29



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