PHYLOGENY OF ENDEMIC MARINE SPONGE HYATTELLA INTESTINALIS AND CHARACTERIZATION OF ASSOCIATED BACTERIA

M. BABU; SURESH DHANARAJ

doi:10.22159/ijap.2022.v14ti.33

Authors

M. BABU Department of Microbiology, School of Life Science, Vels Institute of Science Technology Studies (VITAS) Pallavaram, Chennai, Tamil Nadu, India
SURESH DHANARAJ Department of Microbiology, School of Life Science, Vels Institute of Science Technology Studies (VITAS) Pallavaram, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ijap.2022.v14ti.33

Keywords:

Sponge, Hyattella intestinalis, Sethubavachatram, Lysinibacillus macroides, 16S rRNA sequence

Abstract

Objective: The main objective of this study was to assess the diversity of culturable bacteria associated with marine water sponges.

Methods: The marine sponge of Hyattella intestinalis was collected from Sethubavachatram, a village panchayat in the Thanjavur district of Tamil Nadu state, India. One gram of the sponge sample was cut into small pieces and serially diluted. spread on Zobell 2216E marine agar medium and incubated at room temperate (RT, 24±2oC) for 48 h. Based on morphological features, colonies were randomly picked and purified using streak plates. The linked bacteria strains were given names based on the initials of the sponge from which they were recovered.

Results: The HIB1, HIB2, and HIB3 were isolated from Hyattella intestinalis. Among the three, HIB1 colonies were found dominant, and further, the biochemical characterization and 16S rRNA sequence was studied. It revealed that the marine sponge Lysinibacillus sp. associated bacteria with HIB1 code.

Conclusion: The HIB1 was closely related to Lysinibacillus macroides with 97.81% homology. The NCBI accession number was MZ695813.

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References

Setyowati EP, Purwantiningsih P, Yulianny Erawan FM, Rahmanti S, Rifka Hanum N, Moeksa Devi NC. Cytotoxic and antimicrobial activities of ethyl acetate extract from fungus Trichoderma reesei strain JCM 2267, Aspergillus flavus strain MC-10-L, Penicillium sp, and Aspergillus fumigatus associated with marine sponge Stylissa flabelliformis. Res J Pharm Technol. 2021;14(10):5126-32. doi: 10.52711/0974-360X.2021.00893.

Suman T, Kaleeswaran B, Titus I, Dam RS, Krishnan P. Antibacterial activity of Pseudoceratina purpurea-a marine sponge from Andaman. Res J Pharm Technol. 2013;6(5):544-9.

Sharmalkumar M, Arunagirinathan N, Anand D, Girivasan KP, Baskar M, Rameshkumar MR. In vitro study on antimicrobial and anticancer activities of marine sponge clathria frondifera associated bacteria. Res J Pharm Technol. 2020;13(8):3753-8. doi: 10.5958/0974-360X.2020.00664.2.

Sudayasa IP, Adryan F, Wahyuni Baru S, Mentarry B, La OMJP NSR, Wa OILK. Effect of extract marine sponge xestospongia sp. and aptos sp. on the plasma IL-1β Level in inflammatory model rats: time-dependent. Res J Pharm Technol. 2020;13(11):5455-8.

Adryan F, Muhammad HM, Andi RWS, Dewo D, Saripuddin, La OMJP SR. Anti-inflammatory activity of ethanol extract of marine sponge Petrosia sp. by suppression the level of tumor necrosis factor-alpha. Res J Pharm Technol. 2021;14(8):4435-9.

Sneha S, Mrunal P, Vemula SJ, Anwesha S, Rao KVB. Isolation, characterization and application of protease enzyme from marine bacteria. Res J Pharm Technol. 2021;14(8):4236-40.

Panchami J, Sandra S, Arya K, Anwesha S, Bhaskara Rao KV. A study on marine bacterial invertase enzyme sourced from Bay of Bengal, West Bengal; Its Isolation and Optimization. Res J Pharm Technol. 2020;13(11):5219-23.

Thakar MA, Saurabh Jha S, Phasinam K, Manne R, Qureshi Y, Hari Babu VV. X ray diffraction (XRD) analysis and evaluation of antioxidant activity of copper oxide nanoparticles synthesized from leaf extract of cissus vitiginea. Mater Today Proc. 2022;51:319-24. doi: 10.1016/j.matpr.2021.05.410.

Sharma A, Raja MKMM, Manne R. Evaluation of physicochemical properties and chemical constituents of rasam, a traditional South Indian soup. J Pharm Res Int. 2021:18-25. doi: 10.9734/jpri/2021/v33i1731302.

Bokam YK, Guntupalli C, Gudhanti SNKR, Kulandaivelu U, Alavala RR, Alla NR. Importance of pharmacists as a front-line warrior in improving medication compliance in Covid 19 patients. IJPS. 2021;83(2):398-401. doi: 10.36468/pharmaceutical-sciences.788.

Victor AD, Prasad M, Mohanasundaram S. Biological active compounds with various medicinal values of strychnos nux-vomica–a pharmacological summary. J Glob Trends Pharm Sci. 2016;7(1):3044-7.

Bharathi P, Elavarasi N, Mohanasundaram S. Studies on rate of biodegradation of vegetable oil (Cocunut oil) by using pseudomonas sp. Int J Environ Biol. 2012;2(2):12-9.

Narendhiran S, Mohanasundaram S, Arun J, Saravanan L, Catherine L, Subathra M. Comparative study in larvicidal efficacy of medicinal plant extracts against Culex quinquefasciatus. Int J Res Plant Sci. 2014;4(1):22-5.

Karthikeyan V, Sivakumar K, Aishwarya G, Mohana Sundaram S. Studies on the larvicidal activity of leucas aspera, vitex negundo and eucalyptus against culex quinquefasciatus collected from coovum river of Chennai, India. Asian J Pharm Sci Clin Res. 2012;5(3):1-4.

Mohanasundaram S, Victor AD, Prasad M, Magesh R, Sivakumar K, Subathra M. Pharmacological analysis of hydroethanolic extract of Senna alata (L.) for in vitro free radical scavenging and cytotoxic activities against Hep G2 cancer cell line. Pak J Pharm Sci. 2019;32(3):931-4.

Mohanasundaram S, Rangarajan N, Sampath V, Porkodi K, Prakash MVD, Monicka N. GC-MS identification of anti-inflammatory and anticancer metabolites in edible milky white mushroom (Calocybe indica) against human breast cancer (MCF-7) cells. Res J Pharm Technol. 2021;14(8):4300-6.

Manasa K, Chitra V. Evaluation of in vitro antioxidant activity of camalexin-a novel anti-parkinson’s agent. Res J Pharm Technol. 2020;13(2):578-82. doi: 10.5958/0974-360X.2020.00109.2.

Kumar A, Mahajan A, Begum Z. Phytochemical screening and in vitro study of free radical scavenging activity of flavonoids of Aloe vera. Res J Pharm Technol. 2020;13(2):593-8. doi: 10.5958/0974-360X.2020.00112.2.

Mohanasundaram S, Rangarajan N, Sampath V, Porkodi K, Pennarasi M. GC-MS and HPLC analysis of antiglycogenolytic and glycogenic compounds in kaempferol 3–O–gentiobioside containing Senna alata L leaves in experimental rats. Transl Metab Syndr Res. 2021;4:10-7. doi: 10.1016/j.tmsr.2021.07.001.

Bharti A, Dheeraj A. In vivo and in vitro investigation of cytotoxic and antitumor activities of polyphenolic leaf extract of hibiscus sabdariffa against, breast cancer cell lines. Res J Pharm Technol. 2019;13(2):615-20.

Zeinab AS, Ahmed MAE, Alaa AG, Mohsen SA, Hanan FA, Habiba AA. In vitro antioxidant, antimicrobial and anticancer activities of banana leaves (Musa acuminata) and olive leaves (Olea europaea L.) as by-products. Res J Pharm Technol. 2020;1.

Victor AD, Mohanasundaram S, Prasad M. Analysis of hydroethanolic extract of Senna alata (L.) to screen bioactive compounds with inhibitory activity on lipid peroxidation, in vitro antibacterial and antidiabetic efficacy. Int J Pharm Sci. 2016;6(1):1360-6.

Venkateshwarlu G, Kanakam VB, Sravan PM, Jimmidi B, Devi PS, Ramchander T. Effects of anti-inflammatory activity of Mimosa pudica. Asian J Pharm Res. Jul-Sep 2011;1(3):69-71.

Mital NM, Desai TR. In vitro anti-inflammatory and anti-arthritic activities of fruits of vernonia anthelmintica willed. (Asteraceae). Asian J Pharm Res 2014 Oct-Dec;4(4):186-8.

Kumar KDJ, Sonia K, Madhan R, Selvakumar K, Kalaichelvan PT. Antiyeast, the antioxidant and anticancer activity of Tribulus terrestris Linn and Bougainvillea spectabilis Linn. Res J Pharm Technol. 2011 Sep;4(9):1483-9.