EVALUATION OF MICROBIOLOGICAL CONTAMINATION PARAMETERS OF TRADITIONAL MEDICINAL PREPARATIONS CONTAINING RED GINGER

DIAH KARTIKA PRATAMI; SHIRLY KUMALA; I GUSTI NYOMAN ARY WIDYASIH; NUR MIFTAHURROHMAH; SAFIRA NAFISA

doi:10.22159/ijap.2022.v14s3.15

Authors

DIAH KARTIKA PRATAMI Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
SHIRLY KUMALA Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
I GUSTI NYOMAN ARY WIDYASIH Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
NUR MIFTAHURROHMAH Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
SAFIRA NAFISA Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s3.15

Keywords:

Red ginger, Traditional medicine, Microbial contamination, Instant powder

Abstract

Objective: The purpose of this study was evaluated the presence or absence of pathogenic microbial contamination in traditional medicinal preparations containing red ginger based on specific microbial tests and evaluating the amount of microbial contamination in traditional medicinal preparations containing red ginger based on the Total Plate Number and Mold Yeast values.

Methods: This research was conducted on instant powdered herbal preparations containing red ginger and internal medicinal liquid containing red ginger. This preparation will be tested for safety and quality requirements including Total Plate Number with Tryptic Soy Agar media, Yeast Mold Number with Saboroud Dextrose Agar media, Enterobacteriaceae Numbers with Violet Red Bile Glucose media, Escherichia coli with Mac Conkey Agar media, Clostridia sp with Reinforced media Medium for Clostridia, Salmonella sp with Rappaport Vasiliadis medium Salmonella Enrichment Broth, and Shigella sp with Xylose Lysine Deoxycholate Agar.

Results: The results of the study for the Total Plate Count and Mold and Yeast values in red ginger instant powder preparations were<10 CFU/gr, for testing the microbes Escherichia coli, Salmonella sp., Clostridia, and Shigella sp. is negative. Then, the results of the research on the medicinal liquid in red ginger for microbial contamination values were<10 CFU/gr, for testing Escherichia coli, Salmonella sp., Clostridia, and Shigella sp. is negative/gr.

Conclusion: Based on the results obtained, it is concluded that herbal preparations containing red ginger meet the safety and quality requirements so that they are safe for consumption.

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References

Mao QQ, Xu XY, Cao SY, Gan RY, Corke H, Beta T. Bioactive compounds and bioactivities of ginger (Zingiber officinale roscoe). Foods. 2019;8(6):1-21. doi: 10.3390/foods8060185, PMID 31151279.

World Health Organization. WHO traditional medicine strategy: 2014-2023. World Health Organization; 2013.

Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules. 2016;21(5):559. doi: 10.3390/molecules21050559, PMID 27136524.

Xiong Y, Gao M, van Duijn B, Choi H, van Horssen F, Wang M. International policies and challenges on the legalization of traditional medicine/herbal medicines in the fight against COVID-19. Pharmacol Res. 2021;166:105472. doi: 10.1016/j.phrs.2021.105472, PMID 33592272.

Indrawati MW. Production of herbal mixes (Jamulacang) instant as an effort to increase immune to prevent covid. Vol. 19; 2020.

Sepriani R. Training on making perfomed herbs jahe instant in the village of Banda Buek The City of Padang. JHA. 2021;2(1):24-32. doi: 10.24036/jha.0201.2021.05.

BPOM RI, Nomor PB. Tahun 2019 Persyaratan Keamanan dan Mutu Obat Tradisional. Badan Pengawas Obat Makanan. 2019;32:1-37.

Kemenkes RI. Farmakope Indonesia edisi V. Jakarta Direktorat Jendral Bina Kefarmasian dan Alat Kesehat Republik Indones. 2014.

Puspitasari E, Pratiwi CD, Wahyuni T. The total plate count of beras kencur herbal medicine in subdistrict market Tulungagung city. Med Lab Anal Sci J. 2019(1):65-70.

Arifan F, Winarni S, Wahyuningsih W, Pudjihastuti I, Broto RW. Total plate count (TPC) analysis of processed ginger on Tlogowungu village. Proceedings of the ICoMA 2018. 2019;167:377-9.

Urooj S, Mirani ZA, Naz S. Impact of seasonal variations on bacterial, yeast and Mold’s count in drinking water collected from Karachi Pakistan. PSM Microbiol. 2018;3:37-42.

Luna-Guevara JJ, Arenas-Hernandez MMP, Martinez de la Pena C, Silva JL, Luna-Guevara ML. The role of pathogenic E. coli in fresh vegetables: behavior, contamination factors, and preventive measures. Int J Microbiol. 2019;2019:2894328. doi: 10.1155/2019/2894328, PMID 31885595.

El-Sheikh R, Hegazy I, Zaghlool E, Ali EAM, Gouda AA. Hydrochemical characteristics and water quality assessment in Abu-zaabal area, Eastern Nile Delta, Egypt. Int J Appl Pharm. 2021;13(6 SE):221-31.

Pereira JM, Leme LM, Perdoncini MRFG, Valderrama P, Março PH. Fast discrimination of milk contaminated with Salmonella sp. Via near-infrared spectroscopy. Food Anal Methods. 2018;11(7):1878-85. doi: 10.1007/s12161-017-1090-0.

Cabral JPS. Water microbiology. Bacterial pathogens and water. Int J Environ Res Public Health. 2010;7(10):3657-703. doi: 10.3390/ijerph7103657, PMID 21139855.

Niyogi SK. Shigellosis. J Microbiol. 2005;43(2):133-43. PMID 15880088.