THE ROLE OF PROPOLIS TETRAGONULA SP. IN OXIDATIVE STRESS AND ITS PROTECTIVE EFFECT AGAINST UV RADIATION ON CELLS

SAFIRA CANDRA ASIH; DIAH KARTIKA PRATAMI; MASAFUHMI YOHDA; YOSUKE FUKUTAMI; AHMAD FARIED; MUHAMAD SAHLAN

doi:10.22159/ijap.2022.v14s3.26

Authors

SAFIRA CANDRA ASIH Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia; Depok, 16424, Indonesia
DIAH KARTIKA PRATAMI Laboratory of Phytochemistry, Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
MASAFUHMI YOHDA Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology; Tokyo, Japan
YOSUKE FUKUTAMI Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology; Tokyo, Japan
AHMAD FARIED Department of Neurosurgery and Oncology and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran; Bandung, Indonesia
MUHAMAD SAHLAN Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia; Depok, 16424, Indonesia, Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI, Depok, 16424, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s3.26

Keywords:

Propolis, Oxidative stress, Anti-oxidant, Free radicals

Abstract

Objective: This report presents a study the anti-oxidant properties of propolis wax from Tetragonula sp. bees by evaluating the protection ability of propolis towards cells from ultraviolet (UV) exposure. The next evaluation was using a literature study and LC-MS (Liquid Chromatography-Mass Spectrometry) to find compounds in propolis that are responsible for anti-oxidative stress activity.

Methods: HEK 293T (Human Embryonic Kidney 293 T) and fibroblast cells line were used. Four tests were performed on the cells, namely: cell proliferation assay using water-soluble tetrazolium salt (WST-8); lactate dehydrogenase (LDH), and free radicals produced on cells test by measuring fluorescence intensity produced by dichlorofluorescein; cell viability through observation using fluorescence microscope on cells stained with Hoechst and propidium iodide (PI); and reactive oxygen assay (ROS) Assay. Before UV exposure, propolis wax was added to the cells in different concentrations. The authors also analyzed the component in propolis wax using LC-MS.

Results: Based on this assessment, it was found that propolis wax successfully protects the cells against UV-induced free radicals’ formation by maintaining the cell proliferation rate, reducing the free radicals produced after UV exposure, and decreasing the number of cell death. Nevertheless, we found that a greater concentration of propolis wax tends to be toxic to the cells. While on the LC-MS results obtained about 83 compounds, in which 35 of them are flavonoid and polyphenols derived compounds with antioxidant properties.

Conclusion: Based on these findings, propolis wax produced Tetragonula sp. can be used as a potential alternative treatment of anti-oxidative stress and anti-free radicals.

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