FORMULATION AND CHARACTERIZATION OF SELF NANO-EMULSIFYING DRUG DELIVERY SYSTEM (SNEDDS) FRACTION OF N-HEXANE: ETHYL ACETATE FROM SESEWANUA LEAF (CLERODENDRUM FRAGRANS WILD.)

ZULFIAYU SAPIUN; ARLAN K. IMRAN; SISILIA TERESIA ROSMALA DEWI; DHEA FADILA MASITA PADE; WIDYAWATI IBRAHIM; ROBERT TUNGADI; WIDY SUSANTI ABDULKADIR; YOS BANNE; YUSNITA RIFAI; SARTINI SARTINI; ANDI DIAN PERMANA; YUSNITA RIFAI; YSRAFIL YSRAFIL; NANGSIH SULASTRI SLAMET

doi:10.22159/ijap.2023v15i2.46365

Authors

ZULFIAYU SAPIUN Department of Pharmacy, Health Polytechnic of Gorontalo, Indonesia, Center of Excellent for Cardiovascular and Endocrine, Health Polytechnic of Gorontalo, Indonesia https://orcid.org/0000-0003-2246-2778
ARLAN K. IMRAN Department of Pharmacy, Health Polytechnic of Gorontalo, Indonesia, Center of Excellent for Cardiovascular and Endocrine, Health Polytechnic of Gorontalo, Indonesia https://orcid.org/0000-0003-1729-3666
SISILIA TERESIA ROSMALA DEWI Department of Pharmacy, Health Polytechnic of Makassar, Indonesia
DHEA FADILA MASITA PADE Department of Pharmacy, Health Polytechnic of Gorontalo, Indonesia
WIDYAWATI IBRAHIM Department of Pharmacy, Health Polytechnic of Gorontalo, Indonesia
ROBERT TUNGADI Department of Pharmacy, State University of Gorontalo, Indonesia
WIDY SUSANTI ABDULKADIR Department of Pharmacy, State University of Gorontalo, Indonesia
YOS BANNE Department of Pharmacy, Health Polytechnic of Manado, Indonesia
YUSNITA RIFAI Department of Pharmacy, Hasanuddin University, Indonesia
SARTINI SARTINI Department of Pharmacy, Hasanuddin University, Indonesia
ANDI DIAN PERMANA Department of Pharmacy, Hasanuddin University, Indonesia
YUSNITA RIFAI Department of Pharmacy, Hasanuddin University, Indonesia
YSRAFIL YSRAFIL Faculty of Medicine, Universitas Palangka Raya, Palangka Raya, Indonesia https://orcid.org/0000-0002-5980-7525
NANGSIH SULASTRI SLAMET Department of Pharmacy, Health Polytechnic of Gorontalo, Indonesia, Center of Excellent for Cardiovascular and Endocrine, Health Polytechnic of Gorontalo, Indonesia https://orcid.org/0000-0002-7515-1175

DOI:

https://doi.org/10.22159/ijap.2023v15i2.46365

Keywords:

Surfactant, Polydispersity index, Particle size, Inflammation, Rheumatoid arthritis

Abstract

Objective: Sesewanua leaves contain alkaloid compounds as antioxidants, and its leaves can be used to formulate SNEDDS dosage forms, which can effectively deliver the medicine.

This study intended to determine the variation of surfactant concentration (Tween 80) and cosurfactant (PEG 400) towards pH, viscosity, nano-emulsion duration and characterization using PSA method (particle size and polydispersity index).

Methods: This study employed a quasi-experimental method and the independent variables in this study were variations in the concentration of surfactant (Tween 80) and cosurfactant (PEG 400), which consist of 3 formulas, such as SFS 1 (6:3), SFS 2 (7:2), and SFS 3 (8:1). The dependent variables in this study including pH, viscosity, nano-emulsion time, particle size and polydispersity index which utilized One Way Anova Post Hoc LSD (p>0.05) and Tamhane (p<0.05) tests as the data analysis.

Results: The pH test SFS1-SFS3 has a pH value of 7.92, 8.30 and 8.35, followed by Viscosity test SFS1-SFS3, which has a viscosity value of 1.00 cP, 1.38 cP and 2.91 cP. Further, the SFS1-SFS3 nano emulsified time test had nano emulsified time in gastric and intestinal fluids 35.18s and 43.96s, 43.54s and 47.13s and 44.00s and 50.29s. Characterization of SFS1-SFS3 particle size in gastric and intestinal fluids 23.9 nm and 23.0 nm, 18.5 nm and 22.7 nm and 19.1 nm and 22.9 nm, while characterization of SFS1-SFS3 polydispersity index in gastric and intestinal fluids were 0.433 and 0.348, 0.451 and 0.440 and 0.568 and 0.462.

Conclusion: The increase of variations in surfactant concentration and decreased cosurfactant significantly affected pH, viscosity, nano-emulsion time, and particle size of SFS preparations. However, the polydispersity index was not considerably affected.

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