FORMULATION OF GARGLE FROM TRIGONA SP. PROPOLIS EXTRACT AND ITS ACTIVITY AGAINST STREPTOCOCCUS MUTANS

INSAN SUNAN KURNIAWANSYAH; ANIS YOHANA CHAERUNISAA; ARIF BUDIMAN

doi:10.22159/ijap.2022.v14s4.PP15

Authors

INSAN SUNAN KURNIAWANSYAH Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, West Java, Indonesia
ANIS YOHANA CHAERUNISAA Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, West Java, Indonesia
ARIF BUDIMAN Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, West Java, Indonesia, Center of Dosage Form Development Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, West Java, Indonesia 45363

DOI:

https://doi.org/10.22159/ijap.2022.v14s4.PP15

Keywords:

Dental caries, Gargle, Trigona sp., Propolis, Streptococcus mutans

Abstract

Objective: In this research, the formulations of a gargle with Trigona sp. propolis extracts as an alternative of dental caries therapy and test its activity against Streptococcus mutans was carried out.

Methods: The experimental laboratory have been carried out with the following stages of work, material collection and processing, extraction of Trigona sp. propolis by maceration with 95% ethanol, phytochemical screening, determination of minimum inhibitory concentration, formulation of the gargle, activity, contact time and stability test of the formulas.

Results: Phytochemical screening showed that Trigona sp. propolis extracts containing flavonoids, polyphenols, quinones, monoterpenoids and sesquiterpenoids. Minimum inhibitory concentration was 0.25% w/v. The formulation of gargle from Trigona sp. propolis extract were a yellow solution with mint odor and taste of mint and sweet. The antibacterial activity of gargle preparation and formulas from the market as a comparison, showed that the formula with a concentration of 1% w/v gave a significant difference to the market preparation. The formulation of gargle from Trigona sp. propolis extract has good inhibition against bacteria S. mutans with a contact time of 60 seconds and is physically stable during the 35 d of storage time.

Conclusion: The formulation of gargle from Trigona sp. propolis extract is an excellent preparation to be developed in the future in the treatment of dental caries.

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